Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/17562 |
Resumo: | The objectives of this study were to determine the effect of molybdenum and magnesium removal from nutrient solution and combined nitrogen additions on the Rhizobium phaseoli-Phaseolus vulgaris symbiosis; to study the effect of different Rhisobium strain inoculants; to identify any possible correlation between different variables studied; and to relate the acetylene reduction assay to the common method of evaluating nitrogen fixation by legumes by measuring total nitrogen in plants using the Kjeldahl method, and through dry weight of plants. The study was conducted in a greenhouse (3 experiments) at Purdue University, Indiana, U.S.A. The main treatment consisted of withholding molybdenum, magnesium, or both, from free Crone's solution plus micronutrients, or adding combined nitro gen to culture media. The variety "Rico 23", from Brazil, was used. Plants 40 days old were harvested in all experiments. Combined nitrogen added to nutrient media in the different experiments increased the dry weight of plants, percent and total nitrogen in the plant tops, but decreased the weight of nodules and the amount of acetylene reduced to ethylene by excised nodules. Nodules produced by one strain of Rhizobium demonstrated high nitrogenase activity even in treatments with nitrogen. Removal of molybdenum from nutrient solution did not affect dry weight of plant, or nodule weight, but decreased percent of nitrogen of plant tops. Neither molybdenum nor magnesium removal from nutrient solution influenced nitrogen fixation by different strains of Rhizobium used when measured by the acetylene reduction assay or amount of nitrogen in plant tops. In the absence of combined nitrogen the ethylene produced by nodules was positively correlated with both total and percent nitrogen in plant tops, and with the fresh nodule weight, while negative correlations between dry weight of plant tops and other variables (dry weight of entire plant, fresh nodule weight, and acetylene reduced) were found. There was no correlation between acetylene reduction by nodule tissue and the dry weight of entire plant. |
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Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosisFatores que afetam a simbiose Rhizobium phaseoli - Phaseolus vulgaris The objectives of this study were to determine the effect of molybdenum and magnesium removal from nutrient solution and combined nitrogen additions on the Rhizobium phaseoli-Phaseolus vulgaris symbiosis; to study the effect of different Rhisobium strain inoculants; to identify any possible correlation between different variables studied; and to relate the acetylene reduction assay to the common method of evaluating nitrogen fixation by legumes by measuring total nitrogen in plants using the Kjeldahl method, and through dry weight of plants. The study was conducted in a greenhouse (3 experiments) at Purdue University, Indiana, U.S.A. The main treatment consisted of withholding molybdenum, magnesium, or both, from free Crone's solution plus micronutrients, or adding combined nitro gen to culture media. The variety "Rico 23", from Brazil, was used. Plants 40 days old were harvested in all experiments. Combined nitrogen added to nutrient media in the different experiments increased the dry weight of plants, percent and total nitrogen in the plant tops, but decreased the weight of nodules and the amount of acetylene reduced to ethylene by excised nodules. Nodules produced by one strain of Rhizobium demonstrated high nitrogenase activity even in treatments with nitrogen. Removal of molybdenum from nutrient solution did not affect dry weight of plant, or nodule weight, but decreased percent of nitrogen of plant tops. Neither molybdenum nor magnesium removal from nutrient solution influenced nitrogen fixation by different strains of Rhizobium used when measured by the acetylene reduction assay or amount of nitrogen in plant tops. In the absence of combined nitrogen the ethylene produced by nodules was positively correlated with both total and percent nitrogen in plant tops, and with the fresh nodule weight, while negative correlations between dry weight of plant tops and other variables (dry weight of entire plant, fresh nodule weight, and acetylene reduced) were found. There was no correlation between acetylene reduction by nodule tissue and the dry weight of entire plant.O trabalho traz uma contribuição ao estudo da simbiose Rhizobium-feijão, tendo como objetivos pesquisar: os efeitos causados à simbiose pela omissão de molibdênio e magnésio e adição de nitrogênio mineral na solução nutritiva e os efeitos causados pela inoculação do feijão com diferentes estirpes de Rhizobium. Coeficientes de correlação entre as diferentes variáveis foram calculados com a finalidade principal de relacionar o novo método de medir a atividade da nitrogenase nos nódulos pela redução do acetileno com o teor de nitrogênio da planta e o peso seco da mesma. Foram feitos três experimentos em casa de vegetação, usando-se areia com solução nutritiva. De modo geral, os tratamentos consistiam na omissão de molibdênio, magnésio, ou ambos, com e sem nitrogênio na solução nutritiva de Crone. Foi usada a variedade Rico 23 cru todos os experimentos. Nos tratamentos em que foi omitido o molibdênio na solução nutritiva as plantas não apresentaram nenhuma modificação no peso seco, ou no peso de nódulos, porém, o nitrogênio percentual da parte aérea das mesmas foi menor em um experimento. A omissão de magnésio influenciou diminuindo o peso seco da parte aérea das plantas. As prováveis deficiências de molibdênio e magnésio no meio de cultura não mostraram nenhum efeito na atividade da nitrogenase medida através da redução do acetileno. Correlação positiva foi encontrada entre os métodos da redução do acetileno e o teor de nitrogênio (total e percentual) da parte aérea, porém, não foi observada nenhuma correlação entre o acetileno reduzido pelos nódulos e o peso seco das plantas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraRuschel, Alaides PuppinReuszer, Herbert W.2014-04-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/17562Pesquisa Agropecuaria Brasileira; v.8, n.8, 1973: Série Agronomia; 287-292Pesquisa Agropecuária Brasileira; v.8, n.8, 1973: Série Agronomia; 287-2921678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/17562/11795info:eu-repo/semantics/openAccess2014-04-15T18:50:53Zoai:ojs.seer.sct.embrapa.br:article/17562Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-04-15T18:50:53Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis Fatores que afetam a simbiose Rhizobium phaseoli - Phaseolus vulgaris |
title |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis |
spellingShingle |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis Ruschel, Alaides Puppin |
title_short |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis |
title_full |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis |
title_fullStr |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis |
title_full_unstemmed |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis |
title_sort |
Factors affecting Rhizobium phaseoli-Phaseolus vulgaris symbiosis |
author |
Ruschel, Alaides Puppin |
author_facet |
Ruschel, Alaides Puppin Reuszer, Herbert W. |
author_role |
author |
author2 |
Reuszer, Herbert W. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Ruschel, Alaides Puppin Reuszer, Herbert W. |
dc.subject.none.fl_str_mv |
|
description |
The objectives of this study were to determine the effect of molybdenum and magnesium removal from nutrient solution and combined nitrogen additions on the Rhizobium phaseoli-Phaseolus vulgaris symbiosis; to study the effect of different Rhisobium strain inoculants; to identify any possible correlation between different variables studied; and to relate the acetylene reduction assay to the common method of evaluating nitrogen fixation by legumes by measuring total nitrogen in plants using the Kjeldahl method, and through dry weight of plants. The study was conducted in a greenhouse (3 experiments) at Purdue University, Indiana, U.S.A. The main treatment consisted of withholding molybdenum, magnesium, or both, from free Crone's solution plus micronutrients, or adding combined nitro gen to culture media. The variety "Rico 23", from Brazil, was used. Plants 40 days old were harvested in all experiments. Combined nitrogen added to nutrient media in the different experiments increased the dry weight of plants, percent and total nitrogen in the plant tops, but decreased the weight of nodules and the amount of acetylene reduced to ethylene by excised nodules. Nodules produced by one strain of Rhizobium demonstrated high nitrogenase activity even in treatments with nitrogen. Removal of molybdenum from nutrient solution did not affect dry weight of plant, or nodule weight, but decreased percent of nitrogen of plant tops. Neither molybdenum nor magnesium removal from nutrient solution influenced nitrogen fixation by different strains of Rhizobium used when measured by the acetylene reduction assay or amount of nitrogen in plant tops. In the absence of combined nitrogen the ethylene produced by nodules was positively correlated with both total and percent nitrogen in plant tops, and with the fresh nodule weight, while negative correlations between dry weight of plant tops and other variables (dry weight of entire plant, fresh nodule weight, and acetylene reduced) were found. There was no correlation between acetylene reduction by nodule tissue and the dry weight of entire plant. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-15 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/17562 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/17562 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/17562/11795 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.8, n.8, 1973: Série Agronomia; 287-292 Pesquisa Agropecuária Brasileira; v.8, n.8, 1973: Série Agronomia; 287-292 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
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Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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pab@sct.embrapa.br || sct.pab@embrapa.br |
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