Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense

Detalhes bibliográficos
Autor(a) principal: Barauna, Alexandre Cardoso
Data de Publicação: 2014
Outros Autores: da Silva, Krisle, Pereira, Gilmara Maria Duarte, Kaminski, Paulo Emílio, Perin, Liamara, Zilli, Jerri Edson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/18987
Resumo: The objective of this work was to isolate and characterize rhizobia from nodules of Centrolobium paraense and to evaluate their symbiotic efficiency. Soil samples collected from four sites of the Roraima Cerrado, Brazil, were used to cultivate C. paraense in order to obtain nodules. Isolates (178) were obtained from 334 nodules after cultivation on medium 79. Twenty‑five isolates belonging to six morphological groups were authenticated using Vigna unguiculata and they were characterized by 16S rRNA. Isolates identified as Bradyrhizobium were further characterized using rpoB gene sequencing. A greenhouse experiment was carried out with C. paraense to test the 18 authenticated isolates. Approximately 90% of the isolates grew slowly in medium 79. The 16S rRNA analysis showed that 14 authenticated isolates belong to the genus Bradyrhizobium, and rpoB indicated they constitute different groups compared to previously described species. Only four of the 11 fast‑growing isolates nodulated V. unguiculata, two of which belong to Rhizobium, and two to Pleomorphomonas, which was not previously reported as a nodulating genus. The Bradyrhizobium isolates ERR 326, ERR 399, and ERR 435 had the highest symbiotic efficiency on C. paraense and showed a contribution similar to the nitrogen treatment. Centrolobium paraense is able to nodulate with different rhizobium species, some of which have not yet been described.
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spelling Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraenseDiversity and nitrogen fixation efficiency of rhizobia isolated from nodules of Centrolobium paraenseBradyrhizobium; Rhizobium; diversidade bacteriana; fixação biológica de nitrogênioBradyrhizobium; Rhizobium; bacteria diversity; biological nitrogen fixationThe objective of this work was to isolate and characterize rhizobia from nodules of Centrolobium paraense and to evaluate their symbiotic efficiency. Soil samples collected from four sites of the Roraima Cerrado, Brazil, were used to cultivate C. paraense in order to obtain nodules. Isolates (178) were obtained from 334 nodules after cultivation on medium 79. Twenty‑five isolates belonging to six morphological groups were authenticated using Vigna unguiculata and they were characterized by 16S rRNA. Isolates identified as Bradyrhizobium were further characterized using rpoB gene sequencing. A greenhouse experiment was carried out with C. paraense to test the 18 authenticated isolates. Approximately 90% of the isolates grew slowly in medium 79. The 16S rRNA analysis showed that 14 authenticated isolates belong to the genus Bradyrhizobium, and rpoB indicated they constitute different groups compared to previously described species. Only four of the 11 fast‑growing isolates nodulated V. unguiculata, two of which belong to Rhizobium, and two to Pleomorphomonas, which was not previously reported as a nodulating genus. The Bradyrhizobium isolates ERR 326, ERR 399, and ERR 435 had the highest symbiotic efficiency on C. paraense and showed a contribution similar to the nitrogen treatment. Centrolobium paraense is able to nodulate with different rhizobium species, some of which have not yet been described.O objetivo deste trabalho foi isolar e caracterizar rizóbios de nódulos de Centrolobium paraense e avaliar sua eficiência simbiótica. Amostras de solo, coletadas de quatro locais do Cerrado de Roraima, foram utilizadas para o cultivo de C. paraense, para obtenção dos nódulos. Os isolados (178) foram obtidos dos 334 nódulos coletados após o cultivo em meio 79. Vinte e cinco isolados, pertencentes a seis grupos morfológicos, foram autenticados com uso de Vigna unguiculata e caracterizados pelo 16S rRNA. Os isolados identificados como Bradyrhizobium foram caracterizados depois pelo sequenciamento do gene rpoB. Um experimento em casa de vegetação foi realizado com C. paraense, para testar os 18 isolados autenticados. Aproximadamente 90% dos isolados mostraram crescimento lento em meio 79. A análise do 16S rRNA mostrou que 14 dos isolados autenticados pertencem ao gênero Bradyrhizobium, e o rpoB indicou que eles constituem grupos diferentes em comparação às espécies já descritas. Somente quatro dos 11 isolados com crescimento rápido nodularam V. unguiculata, dois dos quais pertencentes a Rhizobium e dois a Pleomorphomonas, que não foi relatado anteriormente como gênero nodulífero. Os isolados de Bradyrhizobium ERR 326, ERR 399 e ERR 435 apresentaram a maior eficiência em C. paraense e mostraram contribuição similar ao tratamento nitrogenado. Centrolobium paraense é capaz de nodular com diferentes espécies de rizóbios, algumas das quais ainda não foram descritas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraEmbrapaCapesCNPqBarauna, Alexandre Cardosoda Silva, KrislePereira, Gilmara Maria DuarteKaminski, Paulo EmílioPerin, LiamaraZilli, Jerri Edson2014-06-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/18987Pesquisa Agropecuaria Brasileira; v.49, n.4, abr. 2014; 296-305Pesquisa Agropecuária Brasileira; v.49, n.4, abr. 2014; 296-3051678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/18987/12636https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/18987/11346https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/18987/11347https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/18987/11348info:eu-repo/semantics/openAccess2014-06-11T18:46:47Zoai:ojs.seer.sct.embrapa.br:article/18987Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-06-11T18:46:47Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
Diversity and nitrogen fixation efficiency of rhizobia isolated from nodules of Centrolobium paraense
title Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
spellingShingle Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
Barauna, Alexandre Cardoso
Bradyrhizobium; Rhizobium; diversidade bacteriana; fixação biológica de nitrogênio
Bradyrhizobium; Rhizobium; bacteria diversity; biological nitrogen fixation
title_short Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
title_full Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
title_fullStr Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
title_full_unstemmed Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
title_sort Diversidade e eficiência na fixação do nitrogênio de rizóbios isolados de nódulos de Centrolobium paraense
author Barauna, Alexandre Cardoso
author_facet Barauna, Alexandre Cardoso
da Silva, Krisle
Pereira, Gilmara Maria Duarte
Kaminski, Paulo Emílio
Perin, Liamara
Zilli, Jerri Edson
author_role author
author2 da Silva, Krisle
Pereira, Gilmara Maria Duarte
Kaminski, Paulo Emílio
Perin, Liamara
Zilli, Jerri Edson
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
Embrapa
Capes
CNPq
dc.contributor.author.fl_str_mv Barauna, Alexandre Cardoso
da Silva, Krisle
Pereira, Gilmara Maria Duarte
Kaminski, Paulo Emílio
Perin, Liamara
Zilli, Jerri Edson
dc.subject.por.fl_str_mv Bradyrhizobium; Rhizobium; diversidade bacteriana; fixação biológica de nitrogênio
Bradyrhizobium; Rhizobium; bacteria diversity; biological nitrogen fixation
topic Bradyrhizobium; Rhizobium; diversidade bacteriana; fixação biológica de nitrogênio
Bradyrhizobium; Rhizobium; bacteria diversity; biological nitrogen fixation
description The objective of this work was to isolate and characterize rhizobia from nodules of Centrolobium paraense and to evaluate their symbiotic efficiency. Soil samples collected from four sites of the Roraima Cerrado, Brazil, were used to cultivate C. paraense in order to obtain nodules. Isolates (178) were obtained from 334 nodules after cultivation on medium 79. Twenty‑five isolates belonging to six morphological groups were authenticated using Vigna unguiculata and they were characterized by 16S rRNA. Isolates identified as Bradyrhizobium were further characterized using rpoB gene sequencing. A greenhouse experiment was carried out with C. paraense to test the 18 authenticated isolates. Approximately 90% of the isolates grew slowly in medium 79. The 16S rRNA analysis showed that 14 authenticated isolates belong to the genus Bradyrhizobium, and rpoB indicated they constitute different groups compared to previously described species. Only four of the 11 fast‑growing isolates nodulated V. unguiculata, two of which belong to Rhizobium, and two to Pleomorphomonas, which was not previously reported as a nodulating genus. The Bradyrhizobium isolates ERR 326, ERR 399, and ERR 435 had the highest symbiotic efficiency on C. paraense and showed a contribution similar to the nitrogen treatment. Centrolobium paraense is able to nodulate with different rhizobium species, some of which have not yet been described.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-11
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/18987
url https://seer.sct.embrapa.br/index.php/pab/article/view/18987
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/18987/12636
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/18987/11346
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/18987/11347
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/18987/11348
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.49, n.4, abr. 2014; 296-305
Pesquisa Agropecuária Brasileira; v.49, n.4, abr. 2014; 296-305
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
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reponame_str Pesquisa Agropecuária Brasileira (Online)
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repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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