Evaluation of a mass micropropagation system of cassava varieties

Detalhes bibliográficos
Autor(a) principal: de Oliveira, Roberto Pedroso
Data de Publicação: 2000
Outros Autores: Gomes, Tatiana da Silva, Vilarinhos, Alberto Duarte
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/6028
Resumo: The objective of this work was to evaluate a mass micropropagation system for cassava (Manihot esculenta Crantz). Six varieties were used: Aipim-Rosa, Aipim-Maranhão, Cangaíba, Caravela, Cravela and Pretinha. Routine techniques of the Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical were used for meristem isolation, multiplication and plantlet acclimatization. After 30 days, 78% of meristems developed to plantlets. Independently of the variety and subculture, the average multiplication rate was 2.9, thus allowing the production of 231.2 plantlets per each initial explant, after five subcultures. Callus formation was high (22%), but did not affect the multiplication rate. Leaves presented normal shape, size and color. The average contamination rate (7%), losses by other factors (19%) and root formation (52%) were adequate. There was a strong influence of the genotype on the in vitro development of plantlets. 'Cangaíba' showed the highest multiplication rate, plantlet height and root formation per subculture, respectively of 3.6, 43.7 mm and 77%. The plantlets were easily acclimatized.
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spelling Evaluation of a mass micropropagation system of cassava varietiesAvaliação de um sistema de micropropagação massal de variedades de mandiocaManihot esculenta; plant propagation; tissue culture; in vitro culture.Manihot esculenta; propagação de plantas; cultura de tecidos; cultura in vitroThe objective of this work was to evaluate a mass micropropagation system for cassava (Manihot esculenta Crantz). Six varieties were used: Aipim-Rosa, Aipim-Maranhão, Cangaíba, Caravela, Cravela and Pretinha. Routine techniques of the Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical were used for meristem isolation, multiplication and plantlet acclimatization. After 30 days, 78% of meristems developed to plantlets. Independently of the variety and subculture, the average multiplication rate was 2.9, thus allowing the production of 231.2 plantlets per each initial explant, after five subcultures. Callus formation was high (22%), but did not affect the multiplication rate. Leaves presented normal shape, size and color. The average contamination rate (7%), losses by other factors (19%) and root formation (52%) were adequate. There was a strong influence of the genotype on the in vitro development of plantlets. 'Cangaíba' showed the highest multiplication rate, plantlet height and root formation per subculture, respectively of 3.6, 43.7 mm and 77%. The plantlets were easily acclimatized.O objetivo deste trabalho foi avaliar um sistema de micropropagação massal de variedades de mandioca (Manihot esculenta Crantz). Foram utilizadas as variedades Aipim-Rosa, Aipim-Maranhão, Cangaíba, Caravela, Cravela e Pretinha. Para o isolamento dos meristemas, multiplicação e aclimatização das plântulas, empregaram-se métodos desenvolvidos na Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical. Após 30 dias, obteve-se desenvolvimento e formação de plântulas em 78% dos meristemas. Independentemente da variedade e do subcultivo, a taxa média de multiplicação foi de 2,9, permitindo, assim, a obtenção de 231,2 plântulas por matriz, ao final de cinco subcultivos. Embora a porcentagem de formação de calos tenha sido elevada (22%), não afetou negativamente a taxa de multiplicação. Praticamente não houve estiolamento, e as folhas das plântulas apresentaram desenvolvimento normal quanto a sua forma, tamanho e coloração. Os níveis médios de contaminação (7%), perdas por outros fatores (19%) e formação de raízes (52%) foram satisfatórios. Houve efeito pronunciado do genótipo no desenvolvimento in vitro das plântulas. A 'Cangaíba' apresentou as maiores taxas de multiplicação, de altura das plântulas e de formação de raízes por subcultivo, sendo, em média, de 3,6, 43,7 mm e 77%, respectivamente. As plântulas micropropagadas foram facilmente aclimatizadas.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária Brasileirade Oliveira, Roberto PedrosoGomes, Tatiana da SilvaVilarinhos, Alberto Duarte2000-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/6028Pesquisa Agropecuaria Brasileira; v.35, n.12, dez. 2000; 2329-2334Pesquisa Agropecuária Brasileira; v.35, n.12, dez. 2000; 2329-23341678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/6028/3132info:eu-repo/semantics/openAccess2014-08-20T18:56:49Zoai:ojs.seer.sct.embrapa.br:article/6028Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2014-08-20T18:56:49Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Evaluation of a mass micropropagation system of cassava varieties
Avaliação de um sistema de micropropagação massal de variedades de mandioca
title Evaluation of a mass micropropagation system of cassava varieties
spellingShingle Evaluation of a mass micropropagation system of cassava varieties
de Oliveira, Roberto Pedroso
Manihot esculenta; plant propagation; tissue culture; in vitro culture.
Manihot esculenta; propagação de plantas; cultura de tecidos; cultura in vitro
title_short Evaluation of a mass micropropagation system of cassava varieties
title_full Evaluation of a mass micropropagation system of cassava varieties
title_fullStr Evaluation of a mass micropropagation system of cassava varieties
title_full_unstemmed Evaluation of a mass micropropagation system of cassava varieties
title_sort Evaluation of a mass micropropagation system of cassava varieties
author de Oliveira, Roberto Pedroso
author_facet de Oliveira, Roberto Pedroso
Gomes, Tatiana da Silva
Vilarinhos, Alberto Duarte
author_role author
author2 Gomes, Tatiana da Silva
Vilarinhos, Alberto Duarte
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv de Oliveira, Roberto Pedroso
Gomes, Tatiana da Silva
Vilarinhos, Alberto Duarte
dc.subject.por.fl_str_mv Manihot esculenta; plant propagation; tissue culture; in vitro culture.
Manihot esculenta; propagação de plantas; cultura de tecidos; cultura in vitro
topic Manihot esculenta; plant propagation; tissue culture; in vitro culture.
Manihot esculenta; propagação de plantas; cultura de tecidos; cultura in vitro
description The objective of this work was to evaluate a mass micropropagation system for cassava (Manihot esculenta Crantz). Six varieties were used: Aipim-Rosa, Aipim-Maranhão, Cangaíba, Caravela, Cravela and Pretinha. Routine techniques of the Embrapa-Centro Nacional de Pesquisa de Mandioca e Fruticultura Tropical were used for meristem isolation, multiplication and plantlet acclimatization. After 30 days, 78% of meristems developed to plantlets. Independently of the variety and subculture, the average multiplication rate was 2.9, thus allowing the production of 231.2 plantlets per each initial explant, after five subcultures. Callus formation was high (22%), but did not affect the multiplication rate. Leaves presented normal shape, size and color. The average contamination rate (7%), losses by other factors (19%) and root formation (52%) were adequate. There was a strong influence of the genotype on the in vitro development of plantlets. 'Cangaíba' showed the highest multiplication rate, plantlet height and root formation per subculture, respectively of 3.6, 43.7 mm and 77%. The plantlets were easily acclimatized.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6028
url https://seer.sct.embrapa.br/index.php/pab/article/view/6028
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/6028/3132
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.35, n.12, dez. 2000; 2329-2334
Pesquisa Agropecuária Brasileira; v.35, n.12, dez. 2000; 2329-2334
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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