Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter

Detalhes bibliográficos
Autor(a) principal: Attílio, Lísia Borges
Data de Publicação: 2013
Outros Autores: Mourão Filho, Francisco de Assis Alves, Harakava, Ricardo, Silva, Tatiane Loureiro da, Miyata, Luzia Yuriko, Stipp, Liliane Cristina Libório, Mendes, Beatriz Madalena Januzzi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/14840
Resumo: The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.
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spelling Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoterTransformação genética de laranjas doces com o gene D4E1 dirigido pelo promotor AtPP2Citrus sinensis; antimicrobial peptide; tissue‑specific promoterCitrus sinensis; peptídeo antimicrobiano; promotor tecido‑específicoThe objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.O objetivo deste trabalho foi obter plantas transgênicas de laranja 'Pêra' e 'Valência' por meio do gene D4E1 dirigido pelo promotor Arabidopsis thaliana phloem protein (AtPP2) e quantificar a expressão dos transgenes nos diferentes eventos de transformação. Os experimentos de transformação genética foram realizados com segmentos de epicótilo cocultivados com Agrobacterium tumefaciens. Seis plantas de laranja 'Pêra' e sete plantas de laranja 'Valência' foram confirmadas como distintos eventos transgênicos por meio das técnicas de reação em cadeia da polimerase (PCR) e Southern blot. A expressão dos transgenes foi quantificada por PCR quantitativo em tempo real. Os níveis de expressão do gene D4E1 variam de 5 a 50 vezes entre os diferentes eventos de transformação.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, CAPESAttílio, Lísia BorgesMourão Filho, Francisco de Assis AlvesHarakava, RicardoSilva, Tatiane Loureiro daMiyata, Luzia YurikoStipp, Liliane Cristina LibórioMendes, Beatriz Madalena Januzzi2013-10-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/14840Pesquisa Agropecuaria Brasileira; v.48, n.7, jul. 2013; 741-747Pesquisa Agropecuária Brasileira; v.48, n.7, jul. 2013; 741-7471678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://seer.sct.embrapa.br/index.php/pab/article/view/14840/12250https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9859https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9860https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9861https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9862https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9863info:eu-repo/semantics/openAccess2013-10-31T18:42:04Zoai:ojs.seer.sct.embrapa.br:article/14840Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2013-10-31T18:42:04Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
Transformação genética de laranjas doces com o gene D4E1 dirigido pelo promotor AtPP2
title Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
spellingShingle Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
Attílio, Lísia Borges
Citrus sinensis; antimicrobial peptide; tissue‑specific promoter
Citrus sinensis; peptídeo antimicrobiano; promotor tecido‑específico
title_short Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
title_full Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
title_fullStr Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
title_full_unstemmed Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
title_sort Genetic transformation of sweet oranges with the D4E1 gene driven by the AtPP2 promoter
author Attílio, Lísia Borges
author_facet Attílio, Lísia Borges
Mourão Filho, Francisco de Assis Alves
Harakava, Ricardo
Silva, Tatiane Loureiro da
Miyata, Luzia Yuriko
Stipp, Liliane Cristina Libório
Mendes, Beatriz Madalena Januzzi
author_role author
author2 Mourão Filho, Francisco de Assis Alves
Harakava, Ricardo
Silva, Tatiane Loureiro da
Miyata, Luzia Yuriko
Stipp, Liliane Cristina Libório
Mendes, Beatriz Madalena Januzzi
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv CNPq, CAPES
dc.contributor.author.fl_str_mv Attílio, Lísia Borges
Mourão Filho, Francisco de Assis Alves
Harakava, Ricardo
Silva, Tatiane Loureiro da
Miyata, Luzia Yuriko
Stipp, Liliane Cristina Libório
Mendes, Beatriz Madalena Januzzi
dc.subject.por.fl_str_mv Citrus sinensis; antimicrobial peptide; tissue‑specific promoter
Citrus sinensis; peptídeo antimicrobiano; promotor tecido‑específico
topic Citrus sinensis; antimicrobial peptide; tissue‑specific promoter
Citrus sinensis; peptídeo antimicrobiano; promotor tecido‑específico
description The objective of this work was to produce transgenic 'Pêra' and 'Valência' sweet orange plants using the D4E1 gene driven by the Arabidopsis thaliana phloem protein (AtPP2) promoter and to quantify transgene expression in different transformation events. Genetic transformation experiments were carried out with epicotyl segments co‑cultivated with Agrobacterium tumefaciens. Six plants from 'Pêra' sweet orange and seven plants from 'Valência' sweet orange were confirmed as different transgenic events by means of the polymerase chain reaction (PCR) and the Southern blot techniques. Transgene expression was quantified using real‑time quantitative PCR. D4E1 gene expression levels vary from 5 up to 50 times among different transformation events.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-18
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/14840
url https://seer.sct.embrapa.br/index.php/pab/article/view/14840
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/14840/12250
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9859
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9860
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9861
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9862
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/14840/9863
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.48, n.7, jul. 2013; 741-747
Pesquisa Agropecuária Brasileira; v.48, n.7, jul. 2013; 741-747
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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