Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures

Detalhes bibliográficos
Autor(a) principal: Graminha, Érika Barbosa Neves
Data de Publicação: 2005
Outros Autores: Monteiro, Antonio Carlos, da Silva, Heloísa Cristina, Oliveira, Gilson Pereira, da Costa, Alvimar José
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/7046
Resumo: The objective of the present study was to assess in vitro and in vivo the predatory fungus Arthrobotrys musiformis administered orally, in the form of alginate-microencapsulated conidia, or in natura in liquid medium after passage through the gastrointestinal tract of sheep. Maintenance of the predatory activity of the fungus was evaluated in the in vitro test, and its ability to control parasitic nematodes in naturally infested sheep maintained on pastures was analyzed in the in vivo test. The result of the in vitro test confirmed the viability of A. musiformis (95.5%) even after passage through the gastrointestinal tract. In vivo analysis revealed no difference in the number of eggs per gram between treated and control groups, probably because of the low crowding of the corrals, the insufficient amount and interval time of conidial supplying, permitting dispersal and migration of the larvae in the pasture. However, helminths recovered at autopsy from the tracer animals showed that A. musiformis reduced 50.9% and 57.6% the number of Trichostrongylus colubriformis in the groups treated with microencapsulated conidia and conidia in natura, respectively, and 95% the number of L4 larvae of Haemonchus in the group treated with conidia in natura. Despite the inconclusiveness of the results, A. musiformis was considered a promising agent in the biocontrol of gastrintestinal parasitic nematodes.
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spelling Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pasturesControle de nematóides parasitos gastrintestinais por Arthrobotrys musiformis em ovinos naturalmente infestados mantidos em pastagensbiological control; nematophagous fungi; predacious fungicontrole biológico; fungos nematófagos; fungos predadores The objective of the present study was to assess in vitro and in vivo the predatory fungus Arthrobotrys musiformis administered orally, in the form of alginate-microencapsulated conidia, or in natura in liquid medium after passage through the gastrointestinal tract of sheep. Maintenance of the predatory activity of the fungus was evaluated in the in vitro test, and its ability to control parasitic nematodes in naturally infested sheep maintained on pastures was analyzed in the in vivo test. The result of the in vitro test confirmed the viability of A. musiformis (95.5%) even after passage through the gastrointestinal tract. In vivo analysis revealed no difference in the number of eggs per gram between treated and control groups, probably because of the low crowding of the corrals, the insufficient amount and interval time of conidial supplying, permitting dispersal and migration of the larvae in the pasture. However, helminths recovered at autopsy from the tracer animals showed that A. musiformis reduced 50.9% and 57.6% the number of Trichostrongylus colubriformis in the groups treated with microencapsulated conidia and conidia in natura, respectively, and 95% the number of L4 larvae of Haemonchus in the group treated with conidia in natura. Despite the inconclusiveness of the results, A. musiformis was considered a promising agent in the biocontrol of gastrintestinal parasitic nematodes. Este trabalho objetivou avaliar, in vitro e in vivo, o fungo predador Arthrobotrys musiformis, administrado via oral na forma de conídios microencapsulados em alginato ou in natura em meio líquido, após passagem pelo trato gastrintestinal de ovinos. No teste in vitro, avaliou-se a manutenção da atividade predatória, e no teste in vivo, a capacidade de controlar nematóides parasitos de ovinos naturalmente infectados, mantidos em pastagens. O resultado do teste in vitro confirmou a viabilidade de A. musiformis (95,5%), mesmo após a passagem pelo trato gastrintestinal. Na avaliação in vivo, não houve diferença estatística entre o número de ovos por grama de fezes dos grupos tratados e controle, provavelmente em razão da baixa lotação dos piquetes, da quantidade e periodicidade insuficientes de oferecimento de conídios aos ovinos, o que teria proporcionado dispersão e migração das larvas na pastagem. Contudo, os helmintos recuperados na necropsia dos animais traçadores mostraram que A. musiformis reduziu em 50,9% e 57,6% o número de Trichostrongylus colubriformis dos grupos tratados com conídios microencapsulados e in natura, respectivamente, e em 95% o número de L4 de Haemonchus do grupo tratado com conídios in natura. Embora os resultados não sejam conclusivos, A. musiformis mostrou-se promissor agente no biocontrole de nematóides parasitos gastrintestinais.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraGraminha, Érika Barbosa NevesMonteiro, Antonio Carlosda Silva, Heloísa CristinaOliveira, Gilson Pereirada Costa, Alvimar José2005-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/7046Pesquisa Agropecuaria Brasileira; v.40, n.9, set. 2005; 927-933Pesquisa Agropecuária Brasileira; v.40, n.9, set. 2005; 927-9331678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/7046/4094info:eu-repo/semantics/openAccess2010-09-02T14:17:12Zoai:ojs.seer.sct.embrapa.br:article/7046Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2010-09-02T14:17:12Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
Controle de nematóides parasitos gastrintestinais por Arthrobotrys musiformis em ovinos naturalmente infestados mantidos em pastagens
title Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
spellingShingle Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
Graminha, Érika Barbosa Neves
biological control; nematophagous fungi; predacious fungi
controle biológico; fungos nematófagos; fungos predadores
title_short Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
title_full Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
title_fullStr Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
title_full_unstemmed Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
title_sort Control of gastrintestinal parasitic nematodes by Arthrobotrys musiformis in naturally infested sheep maintained on pastures
author Graminha, Érika Barbosa Neves
author_facet Graminha, Érika Barbosa Neves
Monteiro, Antonio Carlos
da Silva, Heloísa Cristina
Oliveira, Gilson Pereira
da Costa, Alvimar José
author_role author
author2 Monteiro, Antonio Carlos
da Silva, Heloísa Cristina
Oliveira, Gilson Pereira
da Costa, Alvimar José
author2_role author
author
author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv Graminha, Érika Barbosa Neves
Monteiro, Antonio Carlos
da Silva, Heloísa Cristina
Oliveira, Gilson Pereira
da Costa, Alvimar José
dc.subject.por.fl_str_mv biological control; nematophagous fungi; predacious fungi
controle biológico; fungos nematófagos; fungos predadores
topic biological control; nematophagous fungi; predacious fungi
controle biológico; fungos nematófagos; fungos predadores
description The objective of the present study was to assess in vitro and in vivo the predatory fungus Arthrobotrys musiformis administered orally, in the form of alginate-microencapsulated conidia, or in natura in liquid medium after passage through the gastrointestinal tract of sheep. Maintenance of the predatory activity of the fungus was evaluated in the in vitro test, and its ability to control parasitic nematodes in naturally infested sheep maintained on pastures was analyzed in the in vivo test. The result of the in vitro test confirmed the viability of A. musiformis (95.5%) even after passage through the gastrointestinal tract. In vivo analysis revealed no difference in the number of eggs per gram between treated and control groups, probably because of the low crowding of the corrals, the insufficient amount and interval time of conidial supplying, permitting dispersal and migration of the larvae in the pasture. However, helminths recovered at autopsy from the tracer animals showed that A. musiformis reduced 50.9% and 57.6% the number of Trichostrongylus colubriformis in the groups treated with microencapsulated conidia and conidia in natura, respectively, and 95% the number of L4 larvae of Haemonchus in the group treated with conidia in natura. Despite the inconclusiveness of the results, A. musiformis was considered a promising agent in the biocontrol of gastrintestinal parasitic nematodes.
publishDate 2005
dc.date.none.fl_str_mv 2005-09-01
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7046
url https://seer.sct.embrapa.br/index.php/pab/article/view/7046
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/7046/4094
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.40, n.9, set. 2005; 927-933
Pesquisa Agropecuária Brasileira; v.40, n.9, set. 2005; 927-933
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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