Reduction of the severity of apple bitter rot by fruit immersion in chitosan

Detalhes bibliográficos
Autor(a) principal: Felipini, Ricardo Barbosa
Data de Publicação: 2010
Outros Autores: Di Piero, Robson Marcelo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/3113
Resumo: The objective of this work was to evaluate the aplication of chitosan on the control of apple bitter rot in postharvest conditions and its effects on Colletotrichum acutatum and fruit peroxidase activity. Apple fruit previously infected with the pathogen were immersed in chitosan suspensions with different concentrations and pHs. To study some possible action mechanisms, in vitro tests were carried out to evaluate the effect of chitosan on spore germination and mycelial growth of C. acutatum. The capacity of chitosan to induce the synthesis of defense enzymes (peroxidases) was evaluated in fruits by spectrofotometric assay. Different doses and pH of chitosan were found to be effective in the reduction of the disease severity. Chitosan at 10 g L-1 (pH 4) had the most technical viability for the control of the disease and reduced the severity in 26%. Considering the action mechanisms, chitosan did not increase peroxidase activity in the fruits; however, it reduced both spore germination and mycelial growth of the pathogen. Chitosan applied at postharvest is an alternative to reduce bitter rot severity.
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spelling Reduction of the severity of apple bitter rot by fruit immersion in chitosanRedução da severidade da podridão-amarga de maçã em pós-colheita pela imersão de frutos em quitosanaColletotrichum acutatum; antibiosis; natural control; resistance inductionColletotrichum acutatum; antibiose; controle alternativo; indução de resistênciaThe objective of this work was to evaluate the aplication of chitosan on the control of apple bitter rot in postharvest conditions and its effects on Colletotrichum acutatum and fruit peroxidase activity. Apple fruit previously infected with the pathogen were immersed in chitosan suspensions with different concentrations and pHs. To study some possible action mechanisms, in vitro tests were carried out to evaluate the effect of chitosan on spore germination and mycelial growth of C. acutatum. The capacity of chitosan to induce the synthesis of defense enzymes (peroxidases) was evaluated in fruits by spectrofotometric assay. Different doses and pH of chitosan were found to be effective in the reduction of the disease severity. Chitosan at 10 g L-1 (pH 4) had the most technical viability for the control of the disease and reduced the severity in 26%. Considering the action mechanisms, chitosan did not increase peroxidase activity in the fruits; however, it reduced both spore germination and mycelial growth of the pathogen. Chitosan applied at postharvest is an alternative to reduce bitter rot severity.O objetivo deste trabalho foi avaliar a aplicação de quitosana no controle da podridão-amarga da maçã em pós-colheita e seus efeitos sobre Colletotrichum acutatum e a atividade da peroxidase nos frutos. Frutos previamente infectados com o patógeno foram imersos em suspensões de quitosana com diferentes concentrações e pHs. Para estudar possíveis mecanismos de ação envolvidos no controle da doença, foram realizados testes in vitro, para avaliar o efeito da quitosana sobre a germinação de conídios de C. acutatum e sobre o crescimento micelial. Foi avaliada a capacidade da quitosana de induzir a síntese de enzimas relacionadas à defesa da planta (peroxidases), por meio de ensaio espectrofotométrico. Houve efeito de doses e de pH da quitosana sobre a redução da severidade da podridão-amarga em maçã. A suspensão de quitosana a 10 g L-1 e pH 4 foi a mais apropriada tecnicamente para o controle da doença, pois reduziu a severidade em 26%. O polissacarídeo não elevou a atividade de peroxidases nos frutos, mas reduziu a germinação de conídios e o crescimento micelial do patógeno. A quitosana aplicada em pós-colheita é uma medida alternativa aos fungicidas para o manejo da podridão-amarga.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPqCNPqFelipini, Ricardo BarbosaDi Piero, Robson Marcelo2010-12-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/3113Pesquisa Agropecuaria Brasileira; v.44, n.12, dez. 2009; 1591-1597Pesquisa Agropecuária Brasileira; v.44, n.12, dez. 2009; 1591-15971678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/3113/5903https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3113/2660info:eu-repo/semantics/openAccess2012-06-17T11:54:26Zoai:ojs.seer.sct.embrapa.br:article/3113Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:54:26Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Reduction of the severity of apple bitter rot by fruit immersion in chitosan
Redução da severidade da podridão-amarga de maçã em pós-colheita pela imersão de frutos em quitosana
title Reduction of the severity of apple bitter rot by fruit immersion in chitosan
spellingShingle Reduction of the severity of apple bitter rot by fruit immersion in chitosan
Felipini, Ricardo Barbosa
Colletotrichum acutatum; antibiosis; natural control; resistance induction
Colletotrichum acutatum; antibiose; controle alternativo; indução de resistência
title_short Reduction of the severity of apple bitter rot by fruit immersion in chitosan
title_full Reduction of the severity of apple bitter rot by fruit immersion in chitosan
title_fullStr Reduction of the severity of apple bitter rot by fruit immersion in chitosan
title_full_unstemmed Reduction of the severity of apple bitter rot by fruit immersion in chitosan
title_sort Reduction of the severity of apple bitter rot by fruit immersion in chitosan
author Felipini, Ricardo Barbosa
author_facet Felipini, Ricardo Barbosa
Di Piero, Robson Marcelo
author_role author
author2 Di Piero, Robson Marcelo
author2_role author
dc.contributor.none.fl_str_mv CNPq
CNPq
dc.contributor.author.fl_str_mv Felipini, Ricardo Barbosa
Di Piero, Robson Marcelo
dc.subject.por.fl_str_mv Colletotrichum acutatum; antibiosis; natural control; resistance induction
Colletotrichum acutatum; antibiose; controle alternativo; indução de resistência
topic Colletotrichum acutatum; antibiosis; natural control; resistance induction
Colletotrichum acutatum; antibiose; controle alternativo; indução de resistência
description The objective of this work was to evaluate the aplication of chitosan on the control of apple bitter rot in postharvest conditions and its effects on Colletotrichum acutatum and fruit peroxidase activity. Apple fruit previously infected with the pathogen were immersed in chitosan suspensions with different concentrations and pHs. To study some possible action mechanisms, in vitro tests were carried out to evaluate the effect of chitosan on spore germination and mycelial growth of C. acutatum. The capacity of chitosan to induce the synthesis of defense enzymes (peroxidases) was evaluated in fruits by spectrofotometric assay. Different doses and pH of chitosan were found to be effective in the reduction of the disease severity. Chitosan at 10 g L-1 (pH 4) had the most technical viability for the control of the disease and reduced the severity in 26%. Considering the action mechanisms, chitosan did not increase peroxidase activity in the fruits; however, it reduced both spore germination and mycelial growth of the pathogen. Chitosan applied at postharvest is an alternative to reduce bitter rot severity.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-22
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/3113
url https://seer.sct.embrapa.br/index.php/pab/article/view/3113
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/3113/5903
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/3113/2660
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.44, n.12, dez. 2009; 1591-1597
Pesquisa Agropecuária Brasileira; v.44, n.12, dez. 2009; 1591-1597
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
collection Pesquisa Agropecuária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pab@sct.embrapa.br || sct.pab@embrapa.br
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