Ideal culture conditions for Dicyma pulvinata conidia mass production

Detalhes bibliográficos
Autor(a) principal: Melo, Débora Ferreira
Data de Publicação: 2010
Outros Autores: Mello, Sueli Corrêa Marques de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Agropecuária Brasileira (Online)
Texto Completo: https://seer.sct.embrapa.br/index.php/pab/article/view/2342
Resumo: The objective of this work was to set up ideal conditions for conidia mass production of Dicyma pulvinata. Four isolates were compared in terms of their growth and conidia production on various substrates (grains of parboiled rice, common rice, maize and wheat, besides chipped maize and rice husk), temperatures (19, 22, 25, 28 and 31oC), growth containers (aluminum trays, polypropylene bags and Erlenmeyers) and light regimes (continuous darkness, 6 and 12 hours of light/darkness, and continuous light). Temperature effects on conidia germination capacity were also evaluated. The experiments were done in randomized complete block designs, in factorial arrangements (isolates x treatments – substrates, containers, temperatures and light regimes), with four replicates. In general, parboiled rice and polypropylene bags provided the best development of the fungus. Complete darkness and 6 hours of light increased mycelial growth, whereas continuous light favored sporulation. All tested temperatures favored the cultures of the fungus, except 31oC. Temperatures between 19 and 25oC ensure spore germination of more than 76%.
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spelling Ideal culture conditions for Dicyma pulvinata conidia mass productionCondições ideais de cultivo para produção massal de conídios de Dicyma pulvinataMicrocyclus ulei; biocontrol; biofungicide; leaf blight; rubber treeMicrocyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueiraThe objective of this work was to set up ideal conditions for conidia mass production of Dicyma pulvinata. Four isolates were compared in terms of their growth and conidia production on various substrates (grains of parboiled rice, common rice, maize and wheat, besides chipped maize and rice husk), temperatures (19, 22, 25, 28 and 31oC), growth containers (aluminum trays, polypropylene bags and Erlenmeyers) and light regimes (continuous darkness, 6 and 12 hours of light/darkness, and continuous light). Temperature effects on conidia germination capacity were also evaluated. The experiments were done in randomized complete block designs, in factorial arrangements (isolates x treatments – substrates, containers, temperatures and light regimes), with four replicates. In general, parboiled rice and polypropylene bags provided the best development of the fungus. Complete darkness and 6 hours of light increased mycelial growth, whereas continuous light favored sporulation. All tested temperatures favored the cultures of the fungus, except 31oC. Temperatures between 19 and 25oC ensure spore germination of more than 76%.Este trabalho teve como objetivo estabelecer condições ideais para a produção massal de conídios do fungo Dicyma pulvinata. Quatro isolados do fungo foram comparados em termos de seu crescimento e produção de conídios em diferentes substratos (grãos de arroz parboilizado, arroz comum, milho e trigo, além de flocos de milho e casca de arroz), temperaturas (19, 22, 25, 28 e 31oC), recipientes de cultivo (bandejas de alumínio, sacos de polipropileno e Erlenmeyers) e regimes de luz (escuridão contínua, fotoperíodos de 6 e de 12 horas de luz/escuro, e luz contínua). Os efeitos da temperatura sobre a capacidade germinativa dos conídios também foram avaliados. Os experimentos foram conduzidos em delineamento de blocos ao acaso, em arranjos fatoriais (isolados x tratamentos – substratos, recipientes, temperaturas e regimes de luz), com quatro repetições. De modo geral, sacos de polipropileno e arroz parboilizado proporcionaram o melhor desenvolvimento do fungo. Fotoperíodos de 0 e 6 horas de luz favoreceram o crescimento micelial, enquanto que a luz contínua favoreceu a esporulação dos isolados. Todas as temperaturas testadas propiciaram o cultivo do fungo, exceto a de 31oC. Temperaturas entre 19 e 25oC asseguram germinação superior a 76% dos conídios do fungo.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, Embrapa e UnBMelo, Débora FerreiraMello, Sueli Corrêa Marques de2010-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/2342Pesquisa Agropecuaria Brasileira; v.44, n.10, out. 2009; 1232-1238Pesquisa Agropecuária Brasileira; v.44, n.10, out. 2009; 1232-12381678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/2342/5834https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2342/1728info:eu-repo/semantics/openAccess2012-06-17T11:53:40Zoai:ojs.seer.sct.embrapa.br:article/2342Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:53:40Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Ideal culture conditions for Dicyma pulvinata conidia mass production
Condições ideais de cultivo para produção massal de conídios de Dicyma pulvinata
title Ideal culture conditions for Dicyma pulvinata conidia mass production
spellingShingle Ideal culture conditions for Dicyma pulvinata conidia mass production
Melo, Débora Ferreira
Microcyclus ulei; biocontrol; biofungicide; leaf blight; rubber tree
Microcyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueira
title_short Ideal culture conditions for Dicyma pulvinata conidia mass production
title_full Ideal culture conditions for Dicyma pulvinata conidia mass production
title_fullStr Ideal culture conditions for Dicyma pulvinata conidia mass production
title_full_unstemmed Ideal culture conditions for Dicyma pulvinata conidia mass production
title_sort Ideal culture conditions for Dicyma pulvinata conidia mass production
author Melo, Débora Ferreira
author_facet Melo, Débora Ferreira
Mello, Sueli Corrêa Marques de
author_role author
author2 Mello, Sueli Corrêa Marques de
author2_role author
dc.contributor.none.fl_str_mv
CNPq, Embrapa e UnB
dc.contributor.author.fl_str_mv Melo, Débora Ferreira
Mello, Sueli Corrêa Marques de
dc.subject.por.fl_str_mv Microcyclus ulei; biocontrol; biofungicide; leaf blight; rubber tree
Microcyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueira
topic Microcyclus ulei; biocontrol; biofungicide; leaf blight; rubber tree
Microcyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueira
description The objective of this work was to set up ideal conditions for conidia mass production of Dicyma pulvinata. Four isolates were compared in terms of their growth and conidia production on various substrates (grains of parboiled rice, common rice, maize and wheat, besides chipped maize and rice husk), temperatures (19, 22, 25, 28 and 31oC), growth containers (aluminum trays, polypropylene bags and Erlenmeyers) and light regimes (continuous darkness, 6 and 12 hours of light/darkness, and continuous light). Temperature effects on conidia germination capacity were also evaluated. The experiments were done in randomized complete block designs, in factorial arrangements (isolates x treatments – substrates, containers, temperatures and light regimes), with four replicates. In general, parboiled rice and polypropylene bags provided the best development of the fungus. Complete darkness and 6 hours of light increased mycelial growth, whereas continuous light favored sporulation. All tested temperatures favored the cultures of the fungus, except 31oC. Temperatures between 19 and 25oC ensure spore germination of more than 76%.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-03
dc.type.none.fl_str_mv
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/2342
url https://seer.sct.embrapa.br/index.php/pab/article/view/2342
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.sct.embrapa.br/index.php/pab/article/view/2342/5834
https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2342/1728
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
publisher.none.fl_str_mv Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuária Brasileira
dc.source.none.fl_str_mv Pesquisa Agropecuaria Brasileira; v.44, n.10, out. 2009; 1232-1238
Pesquisa Agropecuária Brasileira; v.44, n.10, out. 2009; 1232-1238
1678-3921
0100-104x
reponame:Pesquisa Agropecuária Brasileira (Online)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Pesquisa Agropecuária Brasileira (Online)
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repository.name.fl_str_mv Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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