Ideal culture conditions for Dicyma pulvinata conidia mass production
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Pesquisa Agropecuária Brasileira (Online) |
Texto Completo: | https://seer.sct.embrapa.br/index.php/pab/article/view/2342 |
Resumo: | The objective of this work was to set up ideal conditions for conidia mass production of Dicyma pulvinata. Four isolates were compared in terms of their growth and conidia production on various substrates (grains of parboiled rice, common rice, maize and wheat, besides chipped maize and rice husk), temperatures (19, 22, 25, 28 and 31oC), growth containers (aluminum trays, polypropylene bags and Erlenmeyers) and light regimes (continuous darkness, 6 and 12 hours of light/darkness, and continuous light). Temperature effects on conidia germination capacity were also evaluated. The experiments were done in randomized complete block designs, in factorial arrangements (isolates x treatments – substrates, containers, temperatures and light regimes), with four replicates. In general, parboiled rice and polypropylene bags provided the best development of the fungus. Complete darkness and 6 hours of light increased mycelial growth, whereas continuous light favored sporulation. All tested temperatures favored the cultures of the fungus, except 31oC. Temperatures between 19 and 25oC ensure spore germination of more than 76%. |
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Ideal culture conditions for Dicyma pulvinata conidia mass productionCondições ideais de cultivo para produção massal de conídios de Dicyma pulvinataMicrocyclus ulei; biocontrol; biofungicide; leaf blight; rubber treeMicrocyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueiraThe objective of this work was to set up ideal conditions for conidia mass production of Dicyma pulvinata. Four isolates were compared in terms of their growth and conidia production on various substrates (grains of parboiled rice, common rice, maize and wheat, besides chipped maize and rice husk), temperatures (19, 22, 25, 28 and 31oC), growth containers (aluminum trays, polypropylene bags and Erlenmeyers) and light regimes (continuous darkness, 6 and 12 hours of light/darkness, and continuous light). Temperature effects on conidia germination capacity were also evaluated. The experiments were done in randomized complete block designs, in factorial arrangements (isolates x treatments – substrates, containers, temperatures and light regimes), with four replicates. In general, parboiled rice and polypropylene bags provided the best development of the fungus. Complete darkness and 6 hours of light increased mycelial growth, whereas continuous light favored sporulation. All tested temperatures favored the cultures of the fungus, except 31oC. Temperatures between 19 and 25oC ensure spore germination of more than 76%.Este trabalho teve como objetivo estabelecer condições ideais para a produção massal de conídios do fungo Dicyma pulvinata. Quatro isolados do fungo foram comparados em termos de seu crescimento e produção de conídios em diferentes substratos (grãos de arroz parboilizado, arroz comum, milho e trigo, além de flocos de milho e casca de arroz), temperaturas (19, 22, 25, 28 e 31oC), recipientes de cultivo (bandejas de alumínio, sacos de polipropileno e Erlenmeyers) e regimes de luz (escuridão contínua, fotoperíodos de 6 e de 12 horas de luz/escuro, e luz contínua). Os efeitos da temperatura sobre a capacidade germinativa dos conídios também foram avaliados. Os experimentos foram conduzidos em delineamento de blocos ao acaso, em arranjos fatoriais (isolados x tratamentos – substratos, recipientes, temperaturas e regimes de luz), com quatro repetições. De modo geral, sacos de polipropileno e arroz parboilizado proporcionaram o melhor desenvolvimento do fungo. Fotoperíodos de 0 e 6 horas de luz favoreceram o crescimento micelial, enquanto que a luz contínua favoreceu a esporulação dos isolados. Todas as temperaturas testadas propiciaram o cultivo do fungo, exceto a de 31oC. Temperaturas entre 19 e 25oC asseguram germinação superior a 76% dos conídios do fungo.Pesquisa Agropecuaria BrasileiraPesquisa Agropecuária BrasileiraCNPq, Embrapa e UnBMelo, Débora FerreiraMello, Sueli Corrêa Marques de2010-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.sct.embrapa.br/index.php/pab/article/view/2342Pesquisa Agropecuaria Brasileira; v.44, n.10, out. 2009; 1232-1238Pesquisa Agropecuária Brasileira; v.44, n.10, out. 2009; 1232-12381678-39210100-104xreponame:Pesquisa Agropecuária Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://seer.sct.embrapa.br/index.php/pab/article/view/2342/5834https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2342/1728info:eu-repo/semantics/openAccess2012-06-17T11:53:40Zoai:ojs.seer.sct.embrapa.br:article/2342Revistahttp://seer.sct.embrapa.br/index.php/pabPRIhttps://old.scielo.br/oai/scielo-oai.phppab@sct.embrapa.br || sct.pab@embrapa.br1678-39210100-204Xopendoar:2012-06-17T11:53:40Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Ideal culture conditions for Dicyma pulvinata conidia mass production Condições ideais de cultivo para produção massal de conídios de Dicyma pulvinata |
title |
Ideal culture conditions for Dicyma pulvinata conidia mass production |
spellingShingle |
Ideal culture conditions for Dicyma pulvinata conidia mass production Melo, Débora Ferreira Microcyclus ulei; biocontrol; biofungicide; leaf blight; rubber tree Microcyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueira |
title_short |
Ideal culture conditions for Dicyma pulvinata conidia mass production |
title_full |
Ideal culture conditions for Dicyma pulvinata conidia mass production |
title_fullStr |
Ideal culture conditions for Dicyma pulvinata conidia mass production |
title_full_unstemmed |
Ideal culture conditions for Dicyma pulvinata conidia mass production |
title_sort |
Ideal culture conditions for Dicyma pulvinata conidia mass production |
author |
Melo, Débora Ferreira |
author_facet |
Melo, Débora Ferreira Mello, Sueli Corrêa Marques de |
author_role |
author |
author2 |
Mello, Sueli Corrêa Marques de |
author2_role |
author |
dc.contributor.none.fl_str_mv |
CNPq, Embrapa e UnB |
dc.contributor.author.fl_str_mv |
Melo, Débora Ferreira Mello, Sueli Corrêa Marques de |
dc.subject.por.fl_str_mv |
Microcyclus ulei; biocontrol; biofungicide; leaf blight; rubber tree Microcyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueira |
topic |
Microcyclus ulei; biocontrol; biofungicide; leaf blight; rubber tree Microcyclus ulei; controle biológico; biofungicida; mal‑das‑folhas; seringueira |
description |
The objective of this work was to set up ideal conditions for conidia mass production of Dicyma pulvinata. Four isolates were compared in terms of their growth and conidia production on various substrates (grains of parboiled rice, common rice, maize and wheat, besides chipped maize and rice husk), temperatures (19, 22, 25, 28 and 31oC), growth containers (aluminum trays, polypropylene bags and Erlenmeyers) and light regimes (continuous darkness, 6 and 12 hours of light/darkness, and continuous light). Temperature effects on conidia germination capacity were also evaluated. The experiments were done in randomized complete block designs, in factorial arrangements (isolates x treatments – substrates, containers, temperatures and light regimes), with four replicates. In general, parboiled rice and polypropylene bags provided the best development of the fungus. Complete darkness and 6 hours of light increased mycelial growth, whereas continuous light favored sporulation. All tested temperatures favored the cultures of the fungus, except 31oC. Temperatures between 19 and 25oC ensure spore germination of more than 76%. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-03 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/2342 |
url |
https://seer.sct.embrapa.br/index.php/pab/article/view/2342 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://seer.sct.embrapa.br/index.php/pab/article/view/2342/5834 https://seer.sct.embrapa.br/index.php/pab/article/downloadSuppFile/2342/1728 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
publisher.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira Pesquisa Agropecuária Brasileira |
dc.source.none.fl_str_mv |
Pesquisa Agropecuaria Brasileira; v.44, n.10, out. 2009; 1232-1238 Pesquisa Agropecuária Brasileira; v.44, n.10, out. 2009; 1232-1238 1678-3921 0100-104x reponame:Pesquisa Agropecuária Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Agropecuária Brasileira (Online) |
collection |
Pesquisa Agropecuária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Agropecuária Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pab@sct.embrapa.br || sct.pab@embrapa.br |
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1793416693840609280 |