Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Florestal Brasileira (Online) |
Texto Completo: | https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1145 |
Resumo: | The objective of this study was to evaluate the effects of two cryoprotectants followed by thawing on the physiological potential of Genipa americana L. seeds. Two experiments testing 12 treatments were conducted, one for each cryoprotectant, both in a factorial scheme of 6 × 2 (cryoprotectant concentrations × thawing methods). We tested 0, 5, 10, 15, 20, and 25% for dimethyl sulfoxide, and 0, 0.25, 0.5, 0.75, 1.0, and 1.25 M for sucrose. The thawing methods were fast (38 °C for 30 min) and slow (25 °C for 4 h). The seeds were immersed in the cryoprotectant solution for 3 h, stored for 120 h, and then thawed. The seeds were then sown in substrate (sand and vermiculite, 1:1). Emergence percentage along with speed index, length, fresh and dry matter mass of seedlings were evaluated. Dimethyl sulfoxide and sucrose can be used as cryoprotectants in G. americana seeds. Thawing should be slow when treating seeds with dimethyl sulfoxide. |
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Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogenTolerância de sementes de jenipapo a crioprotetores e ao descongelamento após imersão em nitrogênio líquidoGenipa americanaGermplasm conservationSeed preservationGenipa americanaConservação de germoplasmaConservação de sementesThe objective of this study was to evaluate the effects of two cryoprotectants followed by thawing on the physiological potential of Genipa americana L. seeds. Two experiments testing 12 treatments were conducted, one for each cryoprotectant, both in a factorial scheme of 6 × 2 (cryoprotectant concentrations × thawing methods). We tested 0, 5, 10, 15, 20, and 25% for dimethyl sulfoxide, and 0, 0.25, 0.5, 0.75, 1.0, and 1.25 M for sucrose. The thawing methods were fast (38 °C for 30 min) and slow (25 °C for 4 h). The seeds were immersed in the cryoprotectant solution for 3 h, stored for 120 h, and then thawed. The seeds were then sown in substrate (sand and vermiculite, 1:1). Emergence percentage along with speed index, length, fresh and dry matter mass of seedlings were evaluated. Dimethyl sulfoxide and sucrose can be used as cryoprotectants in G. americana seeds. Thawing should be slow when treating seeds with dimethyl sulfoxide.Objetivou-se neste trabalho avaliar os efeitos de dois crioprotetores e métodos de descongelamento sobre o potencial fisiológico de sementes de Genipa americana L. Foram realizados dois experimentos, um para cada crioprotetor, sendo testados 12 tratamentos para cada um, ambos em esquema fatorial 6 x 2 (concentrações de crioprotetor x modos de descongelamento). As concentrações de dimetilsulfóxido foram: 0, 5, 10, 15, 20 e 25% e as de sacarose: 0, 0,25, 0,5, 0,75, 1,0 e 1,25 M. Os modos de descongelamento foram o rápido (38 °C em 30 min) e o lento (25 °C em 4 h). As sementes foram imersas por 3 h em solução crioprotetora e em seguida armazenadas por 120 h, sendo posteriormente descongeladas. Em seguida, foram semeadas em substrato (areia + vermiculita 1:1). Avaliou-se a porcentagem e o índice de velocidade de emergência, comprimento e massa de matéria fresca e seca de plântulas. O dimetilsulfóxido e a sacarose podem ser utilizados como crioprotetor em sementes de G. americana. O descongelamento deve ser lento para as sementes tratadas com dimetilsulfóxido.Embrapa Florestas2017-12-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/114510.4336/2017.pfb.37.92.1145Pesquisa Florestal Brasileira; v. 37 n. 92 (2017): out./dez.; 421-428Pesquisa Florestal Brasileira; Vol. 37 No. 92 (2017): out./dez.; 421-4281983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1145/600https://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessSobrinho, Severino de PaivaAlbuquerque, Maria Cristina de Figueiredo eLuz, Petterson Baptista daNeves, Leonarda GrilloCamili, Elisangela Clarete2019-01-02T18:39:22Zoai:pfb.cnpf.embrapa.br/pfb:article/1145Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2019-01-02T18:39:22Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen Tolerância de sementes de jenipapo a crioprotetores e ao descongelamento após imersão em nitrogênio líquido |
title |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen |
spellingShingle |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen Sobrinho, Severino de Paiva Genipa americana Germplasm conservation Seed preservation Genipa americana Conservação de germoplasma Conservação de sementes |
title_short |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen |
title_full |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen |
title_fullStr |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen |
title_full_unstemmed |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen |
title_sort |
Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen |
author |
Sobrinho, Severino de Paiva |
author_facet |
Sobrinho, Severino de Paiva Albuquerque, Maria Cristina de Figueiredo e Luz, Petterson Baptista da Neves, Leonarda Grillo Camili, Elisangela Clarete |
author_role |
author |
author2 |
Albuquerque, Maria Cristina de Figueiredo e Luz, Petterson Baptista da Neves, Leonarda Grillo Camili, Elisangela Clarete |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Sobrinho, Severino de Paiva Albuquerque, Maria Cristina de Figueiredo e Luz, Petterson Baptista da Neves, Leonarda Grillo Camili, Elisangela Clarete |
dc.subject.por.fl_str_mv |
Genipa americana Germplasm conservation Seed preservation Genipa americana Conservação de germoplasma Conservação de sementes |
topic |
Genipa americana Germplasm conservation Seed preservation Genipa americana Conservação de germoplasma Conservação de sementes |
description |
The objective of this study was to evaluate the effects of two cryoprotectants followed by thawing on the physiological potential of Genipa americana L. seeds. Two experiments testing 12 treatments were conducted, one for each cryoprotectant, both in a factorial scheme of 6 × 2 (cryoprotectant concentrations × thawing methods). We tested 0, 5, 10, 15, 20, and 25% for dimethyl sulfoxide, and 0, 0.25, 0.5, 0.75, 1.0, and 1.25 M for sucrose. The thawing methods were fast (38 °C for 30 min) and slow (25 °C for 4 h). The seeds were immersed in the cryoprotectant solution for 3 h, stored for 120 h, and then thawed. The seeds were then sown in substrate (sand and vermiculite, 1:1). Emergence percentage along with speed index, length, fresh and dry matter mass of seedlings were evaluated. Dimethyl sulfoxide and sucrose can be used as cryoprotectants in G. americana seeds. Thawing should be slow when treating seeds with dimethyl sulfoxide. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1145 10.4336/2017.pfb.37.92.1145 |
url |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1145 |
identifier_str_mv |
10.4336/2017.pfb.37.92.1145 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1145/600 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Embrapa Florestas |
publisher.none.fl_str_mv |
Embrapa Florestas |
dc.source.none.fl_str_mv |
Pesquisa Florestal Brasileira; v. 37 n. 92 (2017): out./dez.; 421-428 Pesquisa Florestal Brasileira; Vol. 37 No. 92 (2017): out./dez.; 421-428 1983-2605 1809-3647 reponame:Pesquisa Florestal Brasileira (Online) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Florestal Brasileira (Online) |
collection |
Pesquisa Florestal Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br |
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1783370935833722880 |