In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa

Detalhes bibliográficos
Autor(a) principal: Moritz, Aline
Data de Publicação: 2010
Outros Autores: Degenhardt, Juliana, Dutra, Leonardo Ferreira, Hansel, Fabrício Augusto, Lima, Bruno Henrique de, Franceschi, Cristina do Rosário Batista, Franciscon, Luziane
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Florestal Brasileira (Online)
Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/14
Resumo: Several species of the Lauraceae family are endangered, due to the high value of their woods,and the consequent exploitation of natural populations. Among them, Ocotea porosa, O. odorifera and O.catharinensis, native from South Brazil, have recalcitrant seeds, what makes even more dificult their naturalregeneration. This work aimed to evaluate in vitro seed germination and multiplication of O. odorifera and O.catharinensis in culture medium supplemented with activated charcoal and/or BAP and in vitro seed germinationand multiplication of O. porosa in culture medium supplemented with several concentrations of sucrose. Theeffect of BAP and activated charcoal were tested in the multiplication of O. catharinensis and O. odorifera andthe effect of sucrose concentration (30, 60, 90 ou 120 g.L-1) was tested in the multiplication of O. porosa. Thegermination percentage was higher than 85% for all species. BAP promoted the highest multiplication rates forO. odorifera. O. porosa showed the best results for in vitro germination and multiplication on 60 g.L-1 sucrose.The establishment of O. catharinensis was achieved, but after some weeks, in all media tested, the explantsshowed abnormalities, and finally died.
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spelling In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosaEstabelecimento in vitro de Ocotea odorifera, O. catharinensis e O. porosaBAPactivated charcoalsucroseconservation.BAPcarvão ativadosacaroseconservaçãoSeveral species of the Lauraceae family are endangered, due to the high value of their woods,and the consequent exploitation of natural populations. Among them, Ocotea porosa, O. odorifera and O.catharinensis, native from South Brazil, have recalcitrant seeds, what makes even more dificult their naturalregeneration. This work aimed to evaluate in vitro seed germination and multiplication of O. odorifera and O.catharinensis in culture medium supplemented with activated charcoal and/or BAP and in vitro seed germinationand multiplication of O. porosa in culture medium supplemented with several concentrations of sucrose. Theeffect of BAP and activated charcoal were tested in the multiplication of O. catharinensis and O. odorifera andthe effect of sucrose concentration (30, 60, 90 ou 120 g.L-1) was tested in the multiplication of O. porosa. Thegermination percentage was higher than 85% for all species. BAP promoted the highest multiplication rates forO. odorifera. O. porosa showed the best results for in vitro germination and multiplication on 60 g.L-1 sucrose.The establishment of O. catharinensis was achieved, but after some weeks, in all media tested, the explantsshowed abnormalities, and finally died.Várias espécies da família Lauraceae encontram-se em risco de extinção, devido ao alto valor comercial de suas madeiras e a consequente exploração das reservas naturais. Dentre elas, Ocotea porosa (imbuia), O. odorifera (canela-sassafras) e O. catharinensis (canela-preta) são de grande importância no Sul do Brasil, de onde são originárias. Estas espécies apresentam sementes recalcitrantes, o que dificulta sua regeneração natural. Alem disso, a propagação por meio de estaquia e enxertia apresenta limitações. Este trabalho teve por objetivo avaliar a germinação e multiplicação in vitro das canelas preta e sassafrás na presença de BAP e carvão ativado e a germinação e multiplicação in vitro de imbuia sob diferentes concentrações de sacarose no meio de cultura. Para as canelas preta e sassafrás foram avaliadas duas concentrações de NaClO na desinfestação de embriões (0,1 % e 0,5 %). Na fase de multiplicação, foi avaliado o efeito de BAP e carvão ativado. Para a imbuia, foi avaliada a influência da concentração de sacarose (30, 60, 90 ou 120 g.L-1) na introdução e multiplicação in vitro. A porcentagem de germinação foi superior a 85 % para as três espécies. Para a canela sassafrás, as melhores taxas de multiplicação foram obtidas na presença de 5 μmol.L-1 de BAP. Para a imbuia, a concentração de 60 g.L-1 de sacarose no meio proporcionou as maiores taxas de multiplicação. Apesar de o estabelecimento da canela preta ter sido satisfatório, após algumas semanas no meio de multiplicação os explantes não apresentavam aparência normal e oxidaram. Doi: 10.4336/2009.pfb.59.37Embrapa Florestas2010-02-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/14Pesquisa Florestal Brasileira; n. 59 (2009): jul./dez.; 37Pesquisa Florestal Brasileira; No. 59 (2009): jul./dez.; 371983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/14/42Moritz, AlineDegenhardt, JulianaDutra, Leonardo FerreiraHansel, Fabrício AugustoLima, Bruno Henrique deFranceschi, Cristina do Rosário BatistaFranciscon, Luzianeinfo:eu-repo/semantics/openAccess2017-04-28T14:06:04Zoai:pfb.cnpf.embrapa.br/pfb:article/14Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2017-04-28T14:06:04Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
Estabelecimento in vitro de Ocotea odorifera, O. catharinensis e O. porosa
title In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
spellingShingle In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
Moritz, Aline
BAP
activated charcoal
sucrose
conservation.
BAP
carvão ativado
sacarose
conservação
title_short In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
title_full In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
title_fullStr In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
title_full_unstemmed In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
title_sort In vitro Establishment of Ocotea odorifera, O. catharinensis and O. porosa
author Moritz, Aline
author_facet Moritz, Aline
Degenhardt, Juliana
Dutra, Leonardo Ferreira
Hansel, Fabrício Augusto
Lima, Bruno Henrique de
Franceschi, Cristina do Rosário Batista
Franciscon, Luziane
author_role author
author2 Degenhardt, Juliana
Dutra, Leonardo Ferreira
Hansel, Fabrício Augusto
Lima, Bruno Henrique de
Franceschi, Cristina do Rosário Batista
Franciscon, Luziane
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moritz, Aline
Degenhardt, Juliana
Dutra, Leonardo Ferreira
Hansel, Fabrício Augusto
Lima, Bruno Henrique de
Franceschi, Cristina do Rosário Batista
Franciscon, Luziane
dc.subject.por.fl_str_mv BAP
activated charcoal
sucrose
conservation.
BAP
carvão ativado
sacarose
conservação
topic BAP
activated charcoal
sucrose
conservation.
BAP
carvão ativado
sacarose
conservação
description Several species of the Lauraceae family are endangered, due to the high value of their woods,and the consequent exploitation of natural populations. Among them, Ocotea porosa, O. odorifera and O.catharinensis, native from South Brazil, have recalcitrant seeds, what makes even more dificult their naturalregeneration. This work aimed to evaluate in vitro seed germination and multiplication of O. odorifera and O.catharinensis in culture medium supplemented with activated charcoal and/or BAP and in vitro seed germinationand multiplication of O. porosa in culture medium supplemented with several concentrations of sucrose. Theeffect of BAP and activated charcoal were tested in the multiplication of O. catharinensis and O. odorifera andthe effect of sucrose concentration (30, 60, 90 ou 120 g.L-1) was tested in the multiplication of O. porosa. Thegermination percentage was higher than 85% for all species. BAP promoted the highest multiplication rates forO. odorifera. O. porosa showed the best results for in vitro germination and multiplication on 60 g.L-1 sucrose.The establishment of O. catharinensis was achieved, but after some weeks, in all media tested, the explantsshowed abnormalities, and finally died.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/14
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/14
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/14/42
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; n. 59 (2009): jul./dez.; 37
Pesquisa Florestal Brasileira; No. 59 (2009): jul./dez.; 37
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
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