In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations

Detalhes bibliográficos
Autor(a) principal: Porfírio, Kennedy de Paiva
Data de Publicação: 2019
Outros Autores: Titon, Miranda, Castro, Ana Caroline Macedo de, Pereira, Israel Marinho, Knegt, Rafael Antonius Pfeilsticker de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Pesquisa Florestal Brasileira (Online)
Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1895
Resumo: The purpose of this study was to develop an in vitro multiplication methodology for Xylopia aromatica, aiming the production of seedlings, evaluating culture media and concentrations of more suitable growth regulators. Nodal segments from seedlings produced from seeds and kept in a nursery were used as explants. The Murashige & Skoog (MS) and Wood Plant Medium (WPM) culture media were tested supplemented with 800 mg L-1 of polyvinylpyrrolidone (PVP), 30 g L-1 of sucrose, 0.054 µM of naphthaleneacetic acid (NAA), 6 g L-1  of agar and concentrations of 2.22 and 3.55 µM of benzylaminopurine (BAP) for in vitro multiplication. At 30 days, the number of lateral sprouts emitted and percentage of explants forming callus at the base were evaluated. The highest multiplication rates were obtained on MS medium supplemented with 3.55 µM of BAP. Regarding callus formation, the MS medium promoted greater formation than WPM medium, however it did not compromise the formation of shoots. It was possible to establish the introduction and multiplication phases, thus defining the culture medium and concentrations of growth regulators most suitable for in vitro culture of Xylopia aromatica.
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spelling In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrationsMultiplicação in vitro de Xylopia aromatica em diferentes meios de cultura e concentrações de BAPMicropropagaçãoReguladores de crescimentoAnnonaceaeMicropropagationGrowth regulatorsAnnonaceaeThe purpose of this study was to develop an in vitro multiplication methodology for Xylopia aromatica, aiming the production of seedlings, evaluating culture media and concentrations of more suitable growth regulators. Nodal segments from seedlings produced from seeds and kept in a nursery were used as explants. The Murashige & Skoog (MS) and Wood Plant Medium (WPM) culture media were tested supplemented with 800 mg L-1 of polyvinylpyrrolidone (PVP), 30 g L-1 of sucrose, 0.054 µM of naphthaleneacetic acid (NAA), 6 g L-1  of agar and concentrations of 2.22 and 3.55 µM of benzylaminopurine (BAP) for in vitro multiplication. At 30 days, the number of lateral sprouts emitted and percentage of explants forming callus at the base were evaluated. The highest multiplication rates were obtained on MS medium supplemented with 3.55 µM of BAP. Regarding callus formation, the MS medium promoted greater formation than WPM medium, however it did not compromise the formation of shoots. It was possible to establish the introduction and multiplication phases, thus defining the culture medium and concentrations of growth regulators most suitable for in vitro culture of Xylopia aromatica.Este estudo teve por objetivo desenvolver uma metodologia de multiplicação in vitro para Xylopia aromatica, visando a produção de mudas, avaliando-se meios de cultura e concentrações de reguladores de crescimento mais adequados. Foram utilizados, como explantes, segmentos nodais, provindos de brotações de mudas produzidas via seminal e mantidas em viveiro. Foram testados os meios de cultura Murashige & Skoog (MS) e Wood Plant Medium (WPM) suplementados com 800 mg L-1 de  polivinilpirrolidona (PVP), 30 g L-1 de sacarose, 0,054 µM de ácido naftalenoacético (ANA) e 6 g L-1 de ágar e concentrações de 2,22 e 3,55 µM de benzilaminopurina (BAP) para a multiplicação in vitro. Aos trinta dias, avaliou-se o número de brotações laterais emitidas e o percentual de explantes formando calos na base. As maiores taxas de multiplicação foram obtidas com o meio MS acrescido de 3,55 µM de BAP. Em relação à formação de calos na base dos explantes, o meio MS promoveu maior formação quando comparado ao WPM, no entanto não comprometeu a formação de brotos. Foi possível estabelecer as fases de introdução e multiplicação, definindo assim o meio de cultura e concentrações de reguladores de crescimento mais adequados ao cultivo in vitro de Xylopia aromatica.Embrapa Florestas2019-07-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/189510.4336/2019.pfb.39e201901895Pesquisa Florestal Brasileira; v. 39 (2019)Pesquisa Florestal Brasileira; Vol. 39 (2019)1983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAporhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1895/898https://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessPorfírio, Kennedy de PaivaTiton, MirandaCastro, Ana Caroline Macedo dePereira, Israel MarinhoKnegt, Rafael Antonius Pfeilsticker de2019-12-31T20:35:26Zoai:pfb.cnpf.embrapa.br/pfb:article/1895Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2019-12-31T20:35:26Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
Multiplicação in vitro de Xylopia aromatica em diferentes meios de cultura e concentrações de BAP
title In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
spellingShingle In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
Porfírio, Kennedy de Paiva
Micropropagação
Reguladores de crescimento
Annonaceae
Micropropagation
Growth regulators
Annonaceae
title_short In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
title_full In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
title_fullStr In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
title_full_unstemmed In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
title_sort In vitro multiplication of Xylopia aromatica under different culture media and BAP concentrations
author Porfírio, Kennedy de Paiva
author_facet Porfírio, Kennedy de Paiva
Titon, Miranda
Castro, Ana Caroline Macedo de
Pereira, Israel Marinho
Knegt, Rafael Antonius Pfeilsticker de
author_role author
author2 Titon, Miranda
Castro, Ana Caroline Macedo de
Pereira, Israel Marinho
Knegt, Rafael Antonius Pfeilsticker de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Porfírio, Kennedy de Paiva
Titon, Miranda
Castro, Ana Caroline Macedo de
Pereira, Israel Marinho
Knegt, Rafael Antonius Pfeilsticker de
dc.subject.por.fl_str_mv Micropropagação
Reguladores de crescimento
Annonaceae
Micropropagation
Growth regulators
Annonaceae
topic Micropropagação
Reguladores de crescimento
Annonaceae
Micropropagation
Growth regulators
Annonaceae
description The purpose of this study was to develop an in vitro multiplication methodology for Xylopia aromatica, aiming the production of seedlings, evaluating culture media and concentrations of more suitable growth regulators. Nodal segments from seedlings produced from seeds and kept in a nursery were used as explants. The Murashige & Skoog (MS) and Wood Plant Medium (WPM) culture media were tested supplemented with 800 mg L-1 of polyvinylpyrrolidone (PVP), 30 g L-1 of sucrose, 0.054 µM of naphthaleneacetic acid (NAA), 6 g L-1  of agar and concentrations of 2.22 and 3.55 µM of benzylaminopurine (BAP) for in vitro multiplication. At 30 days, the number of lateral sprouts emitted and percentage of explants forming callus at the base were evaluated. The highest multiplication rates were obtained on MS medium supplemented with 3.55 µM of BAP. Regarding callus formation, the MS medium promoted greater formation than WPM medium, however it did not compromise the formation of shoots. It was possible to establish the introduction and multiplication phases, thus defining the culture medium and concentrations of growth regulators most suitable for in vitro culture of Xylopia aromatica.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1895
10.4336/2019.pfb.39e201901895
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1895
identifier_str_mv 10.4336/2019.pfb.39e201901895
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1895/898
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; v. 39 (2019)
Pesquisa Florestal Brasileira; Vol. 39 (2019)
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
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