Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin

Detalhes bibliográficos
Autor(a) principal: Meneguzzi, Aline
Data de Publicação: 2019
Outros Autores: Konzen, Enéas Ricardo, Navroski, Marcio Carlos, Camargo, Samila Silva, Pereira, Mariane de Oliveira, Rufato, Léo, Lovatel, Queli Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Florestal Brasileira (Online)
DOI: 10.4336/2019.pfb.39e201701550
Texto Completo: https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1550
Resumo: Sequoia sempervirens (D. Don) Endl. is a conifer which produces high-quality wood with potential industrial applications. However, the species shows low germination rates. This study was devoted to optimizing a protocol for shoot multiplication of S. sempervirens through micropropagation. Two genotypes, adapted to southern Brazil, constituted the source of explants for consecutive experiments aiming in vitro multiplication. We used the traditional MS (Murashige & Skoog) culture medium at 50% of its original concentration, supplemented with plant growth regulators under two approaches. For multiplication we combined α-naphtalene acetic acid (NAA) and 6-benzilaminopurine (BAP) at distinct concentrations. In the second experiment, we tested concentrations of three cytokinins types (BAP, kinetin  and 2-isopentheiladenine ). NAA at 0.1 mg L-1 enabled the production of shoots with higher mean length for the main branch. The use of kinetin in low concentrations provided the best performance for shoot multiplication, differing between two genotypes. We outline a recommendation of the most suitable plant growth regulators and their concentration for shoot multiplication of S. sempervirens, which might assist further work aiming at adventitious rooting and acclimatization. 
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spelling Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetinMicropropagação de dois genótipos de Sequoia sempervirens com adição de pequenas concentrações de cinetinaMicropropagaçãoCitocininasCalogêneseMicropropagationCytokininsCallogenesisSequoia sempervirens (D. Don) Endl. is a conifer which produces high-quality wood with potential industrial applications. However, the species shows low germination rates. This study was devoted to optimizing a protocol for shoot multiplication of S. sempervirens through micropropagation. Two genotypes, adapted to southern Brazil, constituted the source of explants for consecutive experiments aiming in vitro multiplication. We used the traditional MS (Murashige & Skoog) culture medium at 50% of its original concentration, supplemented with plant growth regulators under two approaches. For multiplication we combined α-naphtalene acetic acid (NAA) and 6-benzilaminopurine (BAP) at distinct concentrations. In the second experiment, we tested concentrations of three cytokinins types (BAP, kinetin  and 2-isopentheiladenine ). NAA at 0.1 mg L-1 enabled the production of shoots with higher mean length for the main branch. The use of kinetin in low concentrations provided the best performance for shoot multiplication, differing between two genotypes. We outline a recommendation of the most suitable plant growth regulators and their concentration for shoot multiplication of S. sempervirens, which might assist further work aiming at adventitious rooting and acclimatization. Sequoia sempervirens (D. Don) Endl. é uma conífera que produz madeira de elevada qualidade, com potencial de aplicação na indústria. No entanto, a espécie exibe baixas taxas de germinação. Este estudo foi dedicado à otimizar um protocolo para a multiplicação de brotos de S. sempervirens, através da micropropagação. Dois genótipos, adaptados ao Sul do Brasil, foram a fonte de explantes para o estudo da multiplicação in vitro. Utilizou-se o meio de cultura MS (Murashige Skoog) (50% de sua concentração original) complementado com reguladores de crescimento sob duas abordagens. Para a multiplicação, foram combinados ácido α-naftaleno acético (ANA) e 6-benzilaminopurina (BAP), em concentrações distintas. No segundo experimento foram testadas, separadamente, três concentrações de cintinas (BAP, cinetina  e 2-isopentenladenina). ANA , na concentração 0,1 mg L-1, permitiu a produção de brotos com maior comprimento médio do ramo principal. O uso de cinetina, em baixas concentrações, proporcionou o melhor desempenho para a multiplicação de brotos, mas com desempenhos distintos, de acordo com o genótipo. Descrevemos uma recomendação dos reguladores de crescimento de plantas mais adequados e suas concentrações para a multiplicação de brotos de S. sempervirens, o que pode auxiliar trabalhos voltados para enraizamento e aclimatização adventícia.Embrapa Florestas2019-12-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/155010.4336/2019.pfb.39e201701550Pesquisa Florestal Brasileira; v. 39 (2019)Pesquisa Florestal Brasileira; Vol. 39 (2019)1983-26051809-3647reponame:Pesquisa Florestal Brasileira (Online)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPAenghttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1550/904https://creativecommons.org/licenses/by-nc-nd/4.0info:eu-repo/semantics/openAccessMeneguzzi, AlineKonzen, Enéas RicardoNavroski, Marcio CarlosCamargo, Samila SilvaPereira, Mariane de OliveiraRufato, LéoLovatel, Queli Cristina2019-12-31T20:35:26Zoai:pfb.cnpf.embrapa.br/pfb:article/1550Revistahttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/PUBhttps://pfb.cnpf.embrapa.br/pfb/index.php/pfb/oaipfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br1983-26051809-3647opendoar:2019-12-31T20:35:26Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
Micropropagação de dois genótipos de Sequoia sempervirens com adição de pequenas concentrações de cinetina
title Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
spellingShingle Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
Meneguzzi, Aline
Micropropagação
Citocininas
Calogênese
Micropropagation
Cytokinins
Callogenesis
Meneguzzi, Aline
Micropropagação
Citocininas
Calogênese
Micropropagation
Cytokinins
Callogenesis
title_short Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
title_full Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
title_fullStr Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
title_full_unstemmed Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
title_sort Shoot multiplication of two Sequoia sempervirens genotypes with addition of small concentrations of kinetin
author Meneguzzi, Aline
author_facet Meneguzzi, Aline
Meneguzzi, Aline
Konzen, Enéas Ricardo
Navroski, Marcio Carlos
Camargo, Samila Silva
Pereira, Mariane de Oliveira
Rufato, Léo
Lovatel, Queli Cristina
Konzen, Enéas Ricardo
Navroski, Marcio Carlos
Camargo, Samila Silva
Pereira, Mariane de Oliveira
Rufato, Léo
Lovatel, Queli Cristina
author_role author
author2 Konzen, Enéas Ricardo
Navroski, Marcio Carlos
Camargo, Samila Silva
Pereira, Mariane de Oliveira
Rufato, Léo
Lovatel, Queli Cristina
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Meneguzzi, Aline
Konzen, Enéas Ricardo
Navroski, Marcio Carlos
Camargo, Samila Silva
Pereira, Mariane de Oliveira
Rufato, Léo
Lovatel, Queli Cristina
dc.subject.por.fl_str_mv Micropropagação
Citocininas
Calogênese
Micropropagation
Cytokinins
Callogenesis
topic Micropropagação
Citocininas
Calogênese
Micropropagation
Cytokinins
Callogenesis
description Sequoia sempervirens (D. Don) Endl. is a conifer which produces high-quality wood with potential industrial applications. However, the species shows low germination rates. This study was devoted to optimizing a protocol for shoot multiplication of S. sempervirens through micropropagation. Two genotypes, adapted to southern Brazil, constituted the source of explants for consecutive experiments aiming in vitro multiplication. We used the traditional MS (Murashige & Skoog) culture medium at 50% of its original concentration, supplemented with plant growth regulators under two approaches. For multiplication we combined α-naphtalene acetic acid (NAA) and 6-benzilaminopurine (BAP) at distinct concentrations. In the second experiment, we tested concentrations of three cytokinins types (BAP, kinetin  and 2-isopentheiladenine ). NAA at 0.1 mg L-1 enabled the production of shoots with higher mean length for the main branch. The use of kinetin in low concentrations provided the best performance for shoot multiplication, differing between two genotypes. We outline a recommendation of the most suitable plant growth regulators and their concentration for shoot multiplication of S. sempervirens, which might assist further work aiming at adventitious rooting and acclimatization. 
publishDate 2019
dc.date.none.fl_str_mv 2019-12-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1550
10.4336/2019.pfb.39e201701550
url https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1550
identifier_str_mv 10.4336/2019.pfb.39e201701550
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1550/904
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Embrapa Florestas
publisher.none.fl_str_mv Embrapa Florestas
dc.source.none.fl_str_mv Pesquisa Florestal Brasileira; v. 39 (2019)
Pesquisa Florestal Brasileira; Vol. 39 (2019)
1983-2605
1809-3647
reponame:Pesquisa Florestal Brasileira (Online)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Florestal Brasileira (Online)
collection Pesquisa Florestal Brasileira (Online)
repository.name.fl_str_mv Pesquisa Florestal Brasileira (Online) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv pfb@embrapa.br || revista.pfb@gmail.com || patricia.mattos@embrapa.br
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dc.identifier.doi.none.fl_str_mv 10.4336/2019.pfb.39e201701550

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