Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138387 https://doi.org/10.3389/ffunb.2021.654737 |
Resumo: | Abstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and confirmed that because of its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence. |
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Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation.UV-B radiationThermotoleranceTolerânciaFungo EntomógenoFungo Para Controle BiológicoRadiação SolarRaio UltravioletaControle BiológicoMetarhizium robertsiiEntomopathogenic fungiUltraviolet radiationHeat toleranceOxidative stressBiological controlGene expressionAbstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and confirmed that because of its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence.FLÁVIA REGINA SANTOS DA PAIXÃO, Universidad Nacional de La Plata; CARLA HUARTE BONNET, Universidad Nacional de La Plata; CÁRITA SOUZA RIBEIRO-SILVA, UFG; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UFG.PAIXÃO, F. R. S. daHUARTE BONNET, C.RIBEIRO-SILVA, C. de S.MASCARIN, G. M.FERNANDES, E. K. K.PEDRINI, N.2021-12-27T14:01:02Z2021-12-27T14:01:02Z2021-12-272021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Fungal Biology, v. 2, Article 654737, 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138387https://doi.org/10.3389/ffunb.2021.654737enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-12-27T14:01:12Zoai:www.alice.cnptia.embrapa.br:doc/1138387Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-12-27T14:01:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-12-27T14:01:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
title |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
spellingShingle |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. PAIXÃO, F. R. S. da UV-B radiation Thermotolerance Tolerância Fungo Entomógeno Fungo Para Controle Biológico Radiação Solar Raio Ultravioleta Controle Biológico Metarhizium robertsii Entomopathogenic fungi Ultraviolet radiation Heat tolerance Oxidative stress Biological control Gene expression |
title_short |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
title_full |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
title_fullStr |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
title_full_unstemmed |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
title_sort |
Tolerance to abiotic factors of microsclerotia and mycelial pellets from Metarhizium robertsii, and molecular and ultrastructural changes during microsclerotial differentiation. |
author |
PAIXÃO, F. R. S. da |
author_facet |
PAIXÃO, F. R. S. da HUARTE BONNET, C. RIBEIRO-SILVA, C. de S. MASCARIN, G. M. FERNANDES, E. K. K. PEDRINI, N. |
author_role |
author |
author2 |
HUARTE BONNET, C. RIBEIRO-SILVA, C. de S. MASCARIN, G. M. FERNANDES, E. K. K. PEDRINI, N. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
FLÁVIA REGINA SANTOS DA PAIXÃO, Universidad Nacional de La Plata; CARLA HUARTE BONNET, Universidad Nacional de La Plata; CÁRITA SOUZA RIBEIRO-SILVA, UFG; GABRIEL MOURA MASCARIN, CNPMA; ÉVERTON KORT KAMP FERNANDES, UFG. |
dc.contributor.author.fl_str_mv |
PAIXÃO, F. R. S. da HUARTE BONNET, C. RIBEIRO-SILVA, C. de S. MASCARIN, G. M. FERNANDES, E. K. K. PEDRINI, N. |
dc.subject.por.fl_str_mv |
UV-B radiation Thermotolerance Tolerância Fungo Entomógeno Fungo Para Controle Biológico Radiação Solar Raio Ultravioleta Controle Biológico Metarhizium robertsii Entomopathogenic fungi Ultraviolet radiation Heat tolerance Oxidative stress Biological control Gene expression |
topic |
UV-B radiation Thermotolerance Tolerância Fungo Entomógeno Fungo Para Controle Biológico Radiação Solar Raio Ultravioleta Controle Biológico Metarhizium robertsii Entomopathogenic fungi Ultraviolet radiation Heat tolerance Oxidative stress Biological control Gene expression |
description |
Abstract: Metarhizium species fungi are able to produce resistant structures termed microsclerotia, formed by compact and melanized threads of hyphae. These propagules are tolerant to desiccation and produce infective conidia; thus, they are promising candidates to use in biological control programs. In this study, we investigated the tolerance to both ultraviolet B (UV-B) radiation and heat of microsclerotia of Metarhizium robertsii strain ARSEF 2575. We also adapted the liquid medium and culture conditions to obtain mycelial pellets from the same isolate in order to compare these characteristics between both types of propagules. We followed the peroxisome biogenesis and studied the oxidative stress during differentiation from conidia to microsclerotia by transmission electron microscopy after staining with a peroxidase activity marker and by the expression pattern of genes potentially involved in these processes. We found that despite their twice smaller size, microsclerotia exhibited higher dry biomass, yield, and conidial productivity than mycelial pellets, both with and without UV-B and heat stresses. From the 16 genes measured, we found an induction after 96-h differentiation in the oxidative stress marker genes MrcatA, MrcatP, and Mrgpx; the peroxisome biogenesis factors Mrpex5 and Mrpex14/17; and the photoprotection genes Mrlac1 and Mrlac2; and Mrlac3. We concluded that an oxidative stress scenario is induced during microsclerotia differentiation in M. robertsii and confirmed that because of its tolerance to desiccation, heat, and UV-B, this fungal structure could be an excellent candidate for use in biological control of pests under tropical and subtropical climates where heat and UV radiation are detrimental to entomopathogenic fungi survival and persistence. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-27T14:01:02Z 2021-12-27T14:01:02Z 2021-12-27 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Fungal Biology, v. 2, Article 654737, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138387 https://doi.org/10.3389/ffunb.2021.654737 |
identifier_str_mv |
Frontiers in Fungal Biology, v. 2, Article 654737, 2021. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138387 https://doi.org/10.3389/ffunb.2021.654737 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503515651964928 |