Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.

Detalhes bibliográficos
Autor(a) principal: OKINO, C. H.
Data de Publicação: 2018
Outros Autores: MONTASSIER, M. F. de, OLIVEIRA, A. P. de, MONTASSIER, H. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094600
Resumo: A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates.
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spelling Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.BronquiteInfectious bronchitisBronchitisInfectious bronchitis virusBirdsA method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates.CINTIA HIROMI OKINO, CPPSE; Maria de Fátima Silva Montassier, UNESP; Andressa Peres de Oliveira, UNESP; Helio José Montassier, UNESP.OKINO, C. H.MONTASSIER, M. F. deOLIVEIRA, A. P. deMONTASSIER, H. J.2019-01-16T00:25:42Z2019-01-16T00:25:42Z2018-08-2820182019-01-16T00:25:42Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleVeterinary Medical Science, v. 80, p. 725-730, fev. 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094600enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-01-16T00:25:48Zoai:www.alice.cnptia.embrapa.br:doc/1094600Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-01-16T00:25:48falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-01-16T00:25:48Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
title Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
spellingShingle Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
OKINO, C. H.
Bronquite
Infectious bronchitis
Bronchitis
Infectious bronchitis virus
Birds
title_short Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
title_full Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
title_fullStr Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
title_full_unstemmed Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
title_sort Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.
author OKINO, C. H.
author_facet OKINO, C. H.
MONTASSIER, M. F. de
OLIVEIRA, A. P. de
MONTASSIER, H. J.
author_role author
author2 MONTASSIER, M. F. de
OLIVEIRA, A. P. de
MONTASSIER, H. J.
author2_role author
author
author
dc.contributor.none.fl_str_mv CINTIA HIROMI OKINO, CPPSE; Maria de Fátima Silva Montassier, UNESP; Andressa Peres de Oliveira, UNESP; Helio José Montassier, UNESP.
dc.contributor.author.fl_str_mv OKINO, C. H.
MONTASSIER, M. F. de
OLIVEIRA, A. P. de
MONTASSIER, H. J.
dc.subject.por.fl_str_mv Bronquite
Infectious bronchitis
Bronchitis
Infectious bronchitis virus
Birds
topic Bronquite
Infectious bronchitis
Bronchitis
Infectious bronchitis virus
Birds
description A method based on Melting Temperature analysis of Hypervariable regions (HVR) of S1 gene within a RT-qPCR was developed to detect different genotypes of avian infectious bronchitis virus (IBV) and identify the Mass genotype. The method was able to rapidly identify the Mass genotype among IBV field isolates, vaccine attenuated strains and reference M41 strain in allantoic liquid and also directly in tissues. The RT-qPCR developed detected the virus in both tracheal and pulmonary samples from M41-infected or H120-infected birds, in a larger post-infection period compared to detection by standard method of virus isolation. RT-qPCR method tested provided a sensitivity and rapid approach for screening on IBV detection and Mass genotyping from IBV isolates.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-28
2018
2019-01-16T00:25:42Z
2019-01-16T00:25:42Z
2019-01-16T00:25:42Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Veterinary Medical Science, v. 80, p. 725-730, fev. 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094600
identifier_str_mv Veterinary Medical Science, v. 80, p. 725-730, fev. 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094600
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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