Cellular and humoral immune responses during intrathoracic paracoccidioidomycosis in BALB/c mice.

• Autor(a) principal: ALVES, C. C. de S.
• Data de Publicação: 2009
• Outros Autores: AZEVEDO, A. L. S., RODRIGUES, M. F., MACHADO, R. P., SOUZA, M. A., MACHADO, M. A., TEIXEIRA, H. C., FERREIRA, A. P.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/697051; https://doi.org/10.1016/j.cimid.2008.07.006
• topic: IFN-y; IL-10; Antibody response; Intrathoracic route; Paracoccidioides brasiliensis

Paracoccidioidomycosis is a chronic infection that primarily affects the lungs. Here we investigated cellular and humoral immune responses after intrathoracic Paracoccidioidesbrasiliensis infection in BALB/c mice. P. brasiliensis-colony-forming units (CFUs), fungal DNA and granulomas in lungs increased progressively, peaking at day 90 postinfection (p.i.). IFN-γ production was highest on day 15 p.i., declining thereafter. The kinetics of the NO production was similar to that described for IFN-γ. In contrast, IL-10 increased from day 45 p.i. reaching a peak at day 90. Levels of serum IgG1 were higher than IgG2a between days 30 and 90 p.i. 30% of mice died by day 90 p.i. These data indicate that infection with P. brasiliensis by the intrathoracic route shows high IFN-γ and NO production at day 15 p.i., unable to control multiplication of fungi, which appears to be associated with a progressive increase in IL-10 and in the number and complexity of granulomas.