Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041586 |
Resumo: | The objective of this work was the identification and differentiation of Trichogramma exiguum Pinto and Platner species, T. pretiosum Riley, and T. galloi Zucchi using sequences of the ITS2 region of ribosomal DNA. After extracting DNA from the studied species, a PCR reaction was performed, where the amplified samples were subjected to sequencing. The sequences obtained were submitted to a similarity search in GenBank (NCBI - National Center for Biotechnology Information) using the BLAST program, aiming to determine the similarity of these sequences with the species already deposited in the referenced database, and then multiple sequences were aligned using version 2.0 of the ClustalX program. According to the results of the multiple alignments of all sequences obtained, it was possible to observe the differences between the T. pretiosum, T. galloi and T. exiguum species. It was concluded that using the sequences of the ITS2 region of the ribosomal DNA was efficient in the differentiation of the studied Trichogramma species, which suggests a strong inter-specific variation among species. |
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spelling |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA.MicrohymenopteraParasitoidDNA ribossomalDNATrichogrammaThe objective of this work was the identification and differentiation of Trichogramma exiguum Pinto and Platner species, T. pretiosum Riley, and T. galloi Zucchi using sequences of the ITS2 region of ribosomal DNA. After extracting DNA from the studied species, a PCR reaction was performed, where the amplified samples were subjected to sequencing. The sequences obtained were submitted to a similarity search in GenBank (NCBI - National Center for Biotechnology Information) using the BLAST program, aiming to determine the similarity of these sequences with the species already deposited in the referenced database, and then multiple sequences were aligned using version 2.0 of the ClustalX program. According to the results of the multiple alignments of all sequences obtained, it was possible to observe the differences between the T. pretiosum, T. galloi and T. exiguum species. It was concluded that using the sequences of the ITS2 region of the ribosomal DNA was efficient in the differentiation of the studied Trichogramma species, which suggests a strong inter-specific variation among species.N. R. SANTOS, UFPB; RAUL PORFIRIO DE ALMEIDA, CNPA; I. Q. M. PADILHA, UFPB; D. A. M. ARAÚJO, UFPB; A. J. CREÃO-DUARTE, UFPB.SANTOS, N. R.ALMEIDA, R. P. dePADILHA, I. Q. M.ARAÚJO, D. A. M.CREÃO-DUARTE, A. J.2016-03-22T11:11:11Z2016-03-22T11:11:11Z2016-03-2220152016-03-22T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Biology, v. 75, n. 2, p. 391-395, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041586enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:08:07Zoai:www.alice.cnptia.embrapa.br:doc/1041586Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:08:07falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:08:07Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
title |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
spellingShingle |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. SANTOS, N. R. Microhymenoptera Parasitoid DNA ribossomal DNA Trichogramma |
title_short |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
title_full |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
title_fullStr |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
title_full_unstemmed |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
title_sort |
Molecular identification of Trichogramma species from regions in Brazil using the sequencing of the ITS2 region of ribosomal DNA. |
author |
SANTOS, N. R. |
author_facet |
SANTOS, N. R. ALMEIDA, R. P. de PADILHA, I. Q. M. ARAÚJO, D. A. M. CREÃO-DUARTE, A. J. |
author_role |
author |
author2 |
ALMEIDA, R. P. de PADILHA, I. Q. M. ARAÚJO, D. A. M. CREÃO-DUARTE, A. J. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
N. R. SANTOS, UFPB; RAUL PORFIRIO DE ALMEIDA, CNPA; I. Q. M. PADILHA, UFPB; D. A. M. ARAÚJO, UFPB; A. J. CREÃO-DUARTE, UFPB. |
dc.contributor.author.fl_str_mv |
SANTOS, N. R. ALMEIDA, R. P. de PADILHA, I. Q. M. ARAÚJO, D. A. M. CREÃO-DUARTE, A. J. |
dc.subject.por.fl_str_mv |
Microhymenoptera Parasitoid DNA ribossomal DNA Trichogramma |
topic |
Microhymenoptera Parasitoid DNA ribossomal DNA Trichogramma |
description |
The objective of this work was the identification and differentiation of Trichogramma exiguum Pinto and Platner species, T. pretiosum Riley, and T. galloi Zucchi using sequences of the ITS2 region of ribosomal DNA. After extracting DNA from the studied species, a PCR reaction was performed, where the amplified samples were subjected to sequencing. The sequences obtained were submitted to a similarity search in GenBank (NCBI - National Center for Biotechnology Information) using the BLAST program, aiming to determine the similarity of these sequences with the species already deposited in the referenced database, and then multiple sequences were aligned using version 2.0 of the ClustalX program. According to the results of the multiple alignments of all sequences obtained, it was possible to observe the differences between the T. pretiosum, T. galloi and T. exiguum species. It was concluded that using the sequences of the ITS2 region of the ribosomal DNA was efficient in the differentiation of the studied Trichogramma species, which suggests a strong inter-specific variation among species. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2016-03-22T11:11:11Z 2016-03-22T11:11:11Z 2016-03-22 2016-03-22T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Biology, v. 75, n. 2, p. 391-395, 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041586 |
identifier_str_mv |
Brazilian Journal of Biology, v. 75, n. 2, p. 391-395, 2015. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1041586 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503420120399872 |