An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
DOI: | 10.1038/s41598-018-35315-5 |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102149 |
Resumo: | Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR-30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle. |
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An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.MicroRNAsResidual Feed IntakeGado NeloreBeef cattleNelloreSkeletal muscleResidual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR-30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle.Article number: 17072. Na publicação: Adhemar Zerlotini, Luciana C. A. Regitano.PRISCILA SILVA NEUBERN DE OLIVEIRA, Bolsista/Embrapa Pecuária Sudeste; LUIZ L. COUTINHO, USP; POLYANA C. TIZIOTO, NGS Genomic Solutions; ALINE S. M. CESAR, USP; GABRIELLA B. DE OLIVEIRA, USP; WELLISON J. DA S. DINIZ, UFSCar; ANDRESSA O. DE LIMA, UFSCar; JAMES M. REECY, Iowa State University; GERSON B. MOURÃO, USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.OLIVEIRA, P. S. N. deCOUTINHO, L. L.TIZIOTO, P. C.CESAR, A. S. M.OLIVEIRA, G. B. deDINIZ, W, J. da S.LIMA, A. O. deREECY, J. M.MOURÃO, G. B.ZERLOTINI NETO, A.REGITANO, L. C. de A.2018-12-22T23:31:58Z2018-12-22T23:31:58Z2018-12-2020182019-02-05T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientific Reports, v. 8, p. 1-12, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/110214910.1038/s41598-018-35315-5enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-12-22T23:32:04Zoai:www.alice.cnptia.embrapa.br:doc/1102149Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-12-22T23:32:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
title |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
spellingShingle |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. OLIVEIRA, P. S. N. de MicroRNAs Residual Feed Intake Gado Nelore Beef cattle Nellore Skeletal muscle OLIVEIRA, P. S. N. de MicroRNAs Residual Feed Intake Gado Nelore Beef cattle Nellore Skeletal muscle |
title_short |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
title_full |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
title_fullStr |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
title_full_unstemmed |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
title_sort |
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle. |
author |
OLIVEIRA, P. S. N. de |
author_facet |
OLIVEIRA, P. S. N. de OLIVEIRA, P. S. N. de COUTINHO, L. L. TIZIOTO, P. C. CESAR, A. S. M. OLIVEIRA, G. B. de DINIZ, W, J. da S. LIMA, A. O. de REECY, J. M. MOURÃO, G. B. ZERLOTINI NETO, A. REGITANO, L. C. de A. COUTINHO, L. L. TIZIOTO, P. C. CESAR, A. S. M. OLIVEIRA, G. B. de DINIZ, W, J. da S. LIMA, A. O. de REECY, J. M. MOURÃO, G. B. ZERLOTINI NETO, A. REGITANO, L. C. de A. |
author_role |
author |
author2 |
COUTINHO, L. L. TIZIOTO, P. C. CESAR, A. S. M. OLIVEIRA, G. B. de DINIZ, W, J. da S. LIMA, A. O. de REECY, J. M. MOURÃO, G. B. ZERLOTINI NETO, A. REGITANO, L. C. de A. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
PRISCILA SILVA NEUBERN DE OLIVEIRA, Bolsista/Embrapa Pecuária Sudeste; LUIZ L. COUTINHO, USP; POLYANA C. TIZIOTO, NGS Genomic Solutions; ALINE S. M. CESAR, USP; GABRIELLA B. DE OLIVEIRA, USP; WELLISON J. DA S. DINIZ, UFSCar; ANDRESSA O. DE LIMA, UFSCar; JAMES M. REECY, Iowa State University; GERSON B. MOURÃO, USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
dc.contributor.author.fl_str_mv |
OLIVEIRA, P. S. N. de COUTINHO, L. L. TIZIOTO, P. C. CESAR, A. S. M. OLIVEIRA, G. B. de DINIZ, W, J. da S. LIMA, A. O. de REECY, J. M. MOURÃO, G. B. ZERLOTINI NETO, A. REGITANO, L. C. de A. |
dc.subject.por.fl_str_mv |
MicroRNAs Residual Feed Intake Gado Nelore Beef cattle Nellore Skeletal muscle |
topic |
MicroRNAs Residual Feed Intake Gado Nelore Beef cattle Nellore Skeletal muscle |
description |
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR-30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-22T23:31:58Z 2018-12-22T23:31:58Z 2018-12-20 2018 2019-02-05T11:11:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Scientific Reports, v. 8, p. 1-12, 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102149 10.1038/s41598-018-35315-5 |
identifier_str_mv |
Scientific Reports, v. 8, p. 1-12, 2018. 10.1038/s41598-018-35315-5 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1102149 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1822180794404175872 |
dc.identifier.doi.none.fl_str_mv |
10.1038/s41598-018-35315-5 |