An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.

Detalhes bibliográficos
Autor(a) principal: OLIVEIRA, P. S. N. de
Data de Publicação: 2018
Outros Autores: COUTINHO, L. L., TIZIOTO, P. C., CESAR, A. S. M., OLIVEIRA, G. B. de, DINIZ, W, J. da S., LIMA, A. O. de, REECY, J. M., MOURÃO, G. B., ZERLOTINI NETO, A., REGITANO, L. C. de A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
DOI: 10.1038/s41598-018-35315-5
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099755
Resumo: Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR- 30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle.
id EMBR_885ce209c336690f755021356bdc1a5a
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/1099755
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.RFIMiRNAGado NeloreBeef cattleNelloreSkeletal muscleResidual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR- 30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle.Priscila Silva Neubern de Oliveira, Bolsista/Embrapa Pecuária Sudeste; Luiz L. Coutinho, USP; Polyana C. Tizioto, NGS Genomic Solutions; Aline S. M. Cesar, USP; Gabriella B. de Oliveira, USP; Wellison J. da S. Diniz, UFSCAR; Andressa O. de Lima, UFSCAR; James M. Reecy, Iowa State University; Gerson B. Mourão, USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.OLIVEIRA, P. S. N. deCOUTINHO, L. L.TIZIOTO, P. C.CESAR, A. S. M.OLIVEIRA, G. B. deDINIZ, W, J. da S.LIMA, A. O. deREECY, J. M.MOURÃO, G. B.ZERLOTINI NETO, A.REGITANO, L. C. de A.2018-11-25T23:28:06Z2018-11-25T23:28:06Z2018-11-2120192019-11-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientific Reports, v. 8, n. 17072, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/109975510.1038/s41598-018-35315-5enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-11-25T23:28:13Zoai:www.alice.cnptia.embrapa.br:doc/1099755Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-11-25T23:28:13Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
title An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
spellingShingle An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
OLIVEIRA, P. S. N. de
RFI
MiRNA
Gado Nelore
Beef cattle
Nellore
Skeletal muscle
OLIVEIRA, P. S. N. de
RFI
MiRNA
Gado Nelore
Beef cattle
Nellore
Skeletal muscle
title_short An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
title_full An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
title_fullStr An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
title_full_unstemmed An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
title_sort An integrative transcriptome analysis indicates regulatory mRNA-miRNA networks for residual feed intake in Nelore cattle.
author OLIVEIRA, P. S. N. de
author_facet OLIVEIRA, P. S. N. de
OLIVEIRA, P. S. N. de
COUTINHO, L. L.
TIZIOTO, P. C.
CESAR, A. S. M.
OLIVEIRA, G. B. de
DINIZ, W, J. da S.
LIMA, A. O. de
REECY, J. M.
MOURÃO, G. B.
ZERLOTINI NETO, A.
REGITANO, L. C. de A.
COUTINHO, L. L.
TIZIOTO, P. C.
CESAR, A. S. M.
OLIVEIRA, G. B. de
DINIZ, W, J. da S.
LIMA, A. O. de
REECY, J. M.
MOURÃO, G. B.
ZERLOTINI NETO, A.
REGITANO, L. C. de A.
author_role author
author2 COUTINHO, L. L.
TIZIOTO, P. C.
CESAR, A. S. M.
OLIVEIRA, G. B. de
DINIZ, W, J. da S.
LIMA, A. O. de
REECY, J. M.
MOURÃO, G. B.
ZERLOTINI NETO, A.
REGITANO, L. C. de A.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Priscila Silva Neubern de Oliveira, Bolsista/Embrapa Pecuária Sudeste; Luiz L. Coutinho, USP; Polyana C. Tizioto, NGS Genomic Solutions; Aline S. M. Cesar, USP; Gabriella B. de Oliveira, USP; Wellison J. da S. Diniz, UFSCAR; Andressa O. de Lima, UFSCAR; James M. Reecy, Iowa State University; Gerson B. Mourão, USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
dc.contributor.author.fl_str_mv OLIVEIRA, P. S. N. de
COUTINHO, L. L.
TIZIOTO, P. C.
CESAR, A. S. M.
OLIVEIRA, G. B. de
DINIZ, W, J. da S.
LIMA, A. O. de
REECY, J. M.
MOURÃO, G. B.
ZERLOTINI NETO, A.
REGITANO, L. C. de A.
dc.subject.por.fl_str_mv RFI
MiRNA
Gado Nelore
Beef cattle
Nellore
Skeletal muscle
topic RFI
MiRNA
Gado Nelore
Beef cattle
Nellore
Skeletal muscle
description Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient]). Differentially expressed and hub miRNAs such as bta-miR-486, bta-miR-7, bta-miR15a, bta-miR-21, bta-miR 29, btamiR- 30b, bta-miR-106b, bta-miR-199a-3p, bta-miR-204, and bta-miR 296 may have a potential role in variation of RFI. Functional enrichment analysis of differentially expressed (DE) miRNA?s target genes and miRNA?mRNA correlated modules revealed that insulin, lipid, immune system, oxidative stress and muscle development signaling pathways might potentially be involved in RFI in this population. Our study identified DE miRNAs, miRNA - mRNA regulatory networks and hub miRNAs related to RFI. These findings suggest a possible role of miRNAs in regulation of RFI, providing new insights into the potential molecular mechanisms that control feed efficiency in Nelore cattle.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-25T23:28:06Z
2018-11-25T23:28:06Z
2018-11-21
2019
2019-11-29T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Scientific Reports, v. 8, n. 17072, 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099755
10.1038/s41598-018-35315-5
identifier_str_mv Scientific Reports, v. 8, n. 17072, 2018.
10.1038/s41598-018-35315-5
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099755
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1822180794403127296
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-018-35315-5