Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.).
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002262 |
Resumo: | The objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. Three Brazilian wheat genotypes were tested (Toropi, BRS 194 and F1 wheat cross 020037 × 020062), and two other genotypes were used as controls (Bobwhite and Fielder). Spikes containing uninucleated microspores were subjected to cold pretreatment (4°C) for 21 days in the dark. Number of embryos, green and albino plants were recorded for each genotype. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect. Microspore induction medium alone did not promote embryogenesis; ovary co-culture was a necessary step for embryo development and green plant formation, for all genotypes. The F1 wheat cross (020037 × 020062) produced a total of 85 green plants (out of 108 spikes), 64% of which were spontaneous diploids. BRS 194 produced many embryos, exhibiting a good androgenic response, but only a few grew into green plants. Toropi behaved as a recalcitrant genotype, and zero plants were produced. To our knowledge, this is the first report on wheat IMC from Brazilian genotypes resulting in androgenic embryogenesis and plant regeneration. |
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Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.).Microspore cultureWheat breedingBrasilDuplo haplóideMelhoramento Genético VegetalTrigoandrogenesisThe objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. Three Brazilian wheat genotypes were tested (Toropi, BRS 194 and F1 wheat cross 020037 × 020062), and two other genotypes were used as controls (Bobwhite and Fielder). Spikes containing uninucleated microspores were subjected to cold pretreatment (4°C) for 21 days in the dark. Number of embryos, green and albino plants were recorded for each genotype. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect. Microspore induction medium alone did not promote embryogenesis; ovary co-culture was a necessary step for embryo development and green plant formation, for all genotypes. The F1 wheat cross (020037 × 020062) produced a total of 85 green plants (out of 108 spikes), 64% of which were spontaneous diploids. BRS 194 produced many embryos, exhibiting a good androgenic response, but only a few grew into green plants. Toropi behaved as a recalcitrant genotype, and zero plants were produced. To our knowledge, this is the first report on wheat IMC from Brazilian genotypes resulting in androgenic embryogenesis and plant regeneration.SANDRA MARIA MANSUR SCAGLIUSI, CNPT.SCAGLIUSI, S. M. M.2014-12-10T11:11:11Z2014-12-10T11:11:11Z2014-12-1020142015-02-01T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAustralian Journal of Crop Science, v. 8, n. 6, p. 887-894, 2014.1835-2707http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002262enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T01:35:54Zoai:www.alice.cnptia.embrapa.br:doc/1002262Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T01:35:54falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T01:35:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
title |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
spellingShingle |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). SCAGLIUSI, S. M. M. Microspore culture Wheat breeding Brasil Duplo haplóide Melhoramento Genético Vegetal Trigo androgenesis |
title_short |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
title_full |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
title_fullStr |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
title_full_unstemmed |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
title_sort |
Establishing isolated microspore culture to produce doubled haploid plants in Brazilian wheat (Triticum aestivum L.). |
author |
SCAGLIUSI, S. M. M. |
author_facet |
SCAGLIUSI, S. M. M. |
author_role |
author |
dc.contributor.none.fl_str_mv |
SANDRA MARIA MANSUR SCAGLIUSI, CNPT. |
dc.contributor.author.fl_str_mv |
SCAGLIUSI, S. M. M. |
dc.subject.por.fl_str_mv |
Microspore culture Wheat breeding Brasil Duplo haplóide Melhoramento Genético Vegetal Trigo androgenesis |
topic |
Microspore culture Wheat breeding Brasil Duplo haplóide Melhoramento Genético Vegetal Trigo androgenesis |
description |
The objective of this study was to establish an isolated microspore culture (IMC) protocol in wheat (Triticum aestivum L.) for use in genetic studies and to evaluate its potential for routine use in the Brazilian Wheat Breeding Program at Embrapa Wheat. Important steps of the method were identified and plant physiology of microspore mother plants and ovary co-culture were considered as key factors for effective establishment. Three Brazilian wheat genotypes were tested (Toropi, BRS 194 and F1 wheat cross 020037 × 020062), and two other genotypes were used as controls (Bobwhite and Fielder). Spikes containing uninucleated microspores were subjected to cold pretreatment (4°C) for 21 days in the dark. Number of embryos, green and albino plants were recorded for each genotype. The method was successfully established, and several fertile green plants were produced by using tissue culture and responsive controls. However, the results greatly differed among Brazilian wheat genotypes, suggesting a strong genotype-dependent effect. Microspore induction medium alone did not promote embryogenesis; ovary co-culture was a necessary step for embryo development and green plant formation, for all genotypes. The F1 wheat cross (020037 × 020062) produced a total of 85 green plants (out of 108 spikes), 64% of which were spontaneous diploids. BRS 194 produced many embryos, exhibiting a good androgenic response, but only a few grew into green plants. Toropi behaved as a recalcitrant genotype, and zero plants were produced. To our knowledge, this is the first report on wheat IMC from Brazilian genotypes resulting in androgenic embryogenesis and plant regeneration. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-10T11:11:11Z 2014-12-10T11:11:11Z 2014-12-10 2014 2015-02-01T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Australian Journal of Crop Science, v. 8, n. 6, p. 887-894, 2014. 1835-2707 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002262 |
identifier_str_mv |
Australian Journal of Crop Science, v. 8, n. 6, p. 887-894, 2014. 1835-2707 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002262 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503398469402624 |