Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.

Detalhes bibliográficos
Autor(a) principal: KLAFKE, K.
Data de Publicação: 2023
Outros Autores: SANCHES, M. M., SIHLER, W., SOUZA, M. L. de, TONSO, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592
https:// doi.org/10.3390/pathogens12081001
Resumo: ABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L.
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spelling Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.BIOPESTICIDASpodoptera FrugiperdaControle BiológicoBiological controlBiopesticidesSpodopteraSpodoptera frugiperda multiple nucleopolyhedrovirusABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L.KARINA KLAFKE, UNIVERSIDADE DE SÃO PAULO; MARCIO MARTINELLO SANCHES, CNPGC; WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; ALDO TONSO, UNIVERSIDADE DE SÃO PAULO.KLAFKE, K.SANCHES, M. M.SIHLER, W.SOUZA, M. L. deTONSO, A.2023-09-11T19:24:26Z2023-09-11T19:24:26Z2023-09-112023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePathogens, v. 12, article 1001, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592https:// doi.org/10.3390/pathogens12081001enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-11T19:24:26Zoai:www.alice.cnptia.embrapa.br:doc/1156592Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-11T19:24:26falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-11T19:24:26Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
title Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
spellingShingle Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
KLAFKE, K.
BIOPESTICIDA
Spodoptera Frugiperda
Controle Biológico
Biological control
Biopesticides
Spodoptera
Spodoptera frugiperda multiple nucleopolyhedrovirus
title_short Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
title_full Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
title_fullStr Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
title_full_unstemmed Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
title_sort Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
author KLAFKE, K.
author_facet KLAFKE, K.
SANCHES, M. M.
SIHLER, W.
SOUZA, M. L. de
TONSO, A.
author_role author
author2 SANCHES, M. M.
SIHLER, W.
SOUZA, M. L. de
TONSO, A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv KARINA KLAFKE, UNIVERSIDADE DE SÃO PAULO; MARCIO MARTINELLO SANCHES, CNPGC; WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; ALDO TONSO, UNIVERSIDADE DE SÃO PAULO.
dc.contributor.author.fl_str_mv KLAFKE, K.
SANCHES, M. M.
SIHLER, W.
SOUZA, M. L. de
TONSO, A.
dc.subject.por.fl_str_mv BIOPESTICIDA
Spodoptera Frugiperda
Controle Biológico
Biological control
Biopesticides
Spodoptera
Spodoptera frugiperda multiple nucleopolyhedrovirus
topic BIOPESTICIDA
Spodoptera Frugiperda
Controle Biológico
Biological control
Biopesticides
Spodoptera
Spodoptera frugiperda multiple nucleopolyhedrovirus
description ABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-11T19:24:26Z
2023-09-11T19:24:26Z
2023-09-11
2023
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pathogens, v. 12, article 1001, 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592
https:// doi.org/10.3390/pathogens12081001
identifier_str_mv Pathogens, v. 12, article 1001, 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592
https:// doi.org/10.3390/pathogens12081001
dc.language.iso.fl_str_mv eng
language eng
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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