Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592 https:// doi.org/10.3390/pathogens12081001 |
Resumo: | ABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L. |
id |
EMBR_14661a39fe4aa7a17794278e7a49636a |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1156592 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide.BIOPESTICIDASpodoptera FrugiperdaControle BiológicoBiological controlBiopesticidesSpodopteraSpodoptera frugiperda multiple nucleopolyhedrovirusABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L.KARINA KLAFKE, UNIVERSIDADE DE SÃO PAULO; MARCIO MARTINELLO SANCHES, CNPGC; WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; ALDO TONSO, UNIVERSIDADE DE SÃO PAULO.KLAFKE, K.SANCHES, M. M.SIHLER, W.SOUZA, M. L. deTONSO, A.2023-09-11T19:24:26Z2023-09-11T19:24:26Z2023-09-112023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePathogens, v. 12, article 1001, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592https:// doi.org/10.3390/pathogens12081001enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-11T19:24:26Zoai:www.alice.cnptia.embrapa.br:doc/1156592Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-11T19:24:26falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-11T19:24:26Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
title |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
spellingShingle |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. KLAFKE, K. BIOPESTICIDA Spodoptera Frugiperda Controle Biológico Biological control Biopesticides Spodoptera Spodoptera frugiperda multiple nucleopolyhedrovirus |
title_short |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
title_full |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
title_fullStr |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
title_full_unstemmed |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
title_sort |
Bioreactor production process of Spodoptera frugiperda multiple nucleopolyhedrovirus biopesticide. |
author |
KLAFKE, K. |
author_facet |
KLAFKE, K. SANCHES, M. M. SIHLER, W. SOUZA, M. L. de TONSO, A. |
author_role |
author |
author2 |
SANCHES, M. M. SIHLER, W. SOUZA, M. L. de TONSO, A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
KARINA KLAFKE, UNIVERSIDADE DE SÃO PAULO; MARCIO MARTINELLO SANCHES, CNPGC; WILLIAM SIHLER, Cenargen; MARLINDA LOBO DE SOUZA, Cenargen; ALDO TONSO, UNIVERSIDADE DE SÃO PAULO. |
dc.contributor.author.fl_str_mv |
KLAFKE, K. SANCHES, M. M. SIHLER, W. SOUZA, M. L. de TONSO, A. |
dc.subject.por.fl_str_mv |
BIOPESTICIDA Spodoptera Frugiperda Controle Biológico Biological control Biopesticides Spodoptera Spodoptera frugiperda multiple nucleopolyhedrovirus |
topic |
BIOPESTICIDA Spodoptera Frugiperda Controle Biológico Biological control Biopesticides Spodoptera Spodoptera frugiperda multiple nucleopolyhedrovirus |
description |
ABSTRACT - Spodoptera frugiperda (fall armyworm) is one of the most important maize pests in the world and the baculovirus Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells and virus production. The Bioreactor Stirred Tank Reactor (STR) approach with glutamine-supplemented Sf-900 III serum free culture medium, combined with the MOI of 1.0, showed the best viral production performance, with a specific productivity above 300 occlusion bodies (OBs)/cell and volumetric productivity of 9.0 x 10¹¹ OBs/L. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-11T19:24:26Z 2023-09-11T19:24:26Z 2023-09-11 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Pathogens, v. 12, article 1001, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592 https:// doi.org/10.3390/pathogens12081001 |
identifier_str_mv |
Pathogens, v. 12, article 1001, 2023. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156592 https:// doi.org/10.3390/pathogens12081001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503549171793920 |