The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions.
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135457 https://doi.org/10.3184/095422905782774865 |
Resumo: | Size-exclusion chromatography is a powerful tool for distinguishing dissolved organic matter in relation to its molecular size. However, problems may arise in the choice of the most appropriate eluent composition, regarding the gel used. In the present work, soil solution samples from four Brazilian topsoils were analyzed by high-performance liquid chromatography (HPLC) using a size-exclusion polymer-based column (Superdex 75 HR 10/30, Pharmacia). Water and 0.01 mol L-1 NaCl were investigated as mobile phases. The analysis of the UV peak distribution as well as their corresponding Fe, Mn, and Al concentrations suggested that the salt solution should be preferred since it minimizes unwanted interactions between the sample and the support material. Fe, Mn, and Al speciation calculated by the Geochem-PC program involving the Mattigod and Sposito Mixed Model was compared with the Fe, Mn, and Al-organic complex concentrations determined by SEC-HPLC/GFAAS. No correlation was found for Mn and Fe. For Al a significant non-linear correlation was observed. |
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The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions.SEC-HPLCGeochem-PCSize-exclusion chromatography is a powerful tool for distinguishing dissolved organic matter in relation to its molecular size. However, problems may arise in the choice of the most appropriate eluent composition, regarding the gel used. In the present work, soil solution samples from four Brazilian topsoils were analyzed by high-performance liquid chromatography (HPLC) using a size-exclusion polymer-based column (Superdex 75 HR 10/30, Pharmacia). Water and 0.01 mol L-1 NaCl were investigated as mobile phases. The analysis of the UV peak distribution as well as their corresponding Fe, Mn, and Al concentrations suggested that the salt solution should be preferred since it minimizes unwanted interactions between the sample and the support material. Fe, Mn, and Al speciation calculated by the Geochem-PC program involving the Mattigod and Sposito Mixed Model was compared with the Fe, Mn, and Al-organic complex concentrations determined by SEC-HPLC/GFAAS. No correlation was found for Mn and Fe. For Al a significant non-linear correlation was observed.DANIEL VIDAL PEREZ, CNPS; REINALDO C. DE CAMPOS, PUC-RIO.PEREZ, D. V.CAMPOS, R. C. de2021-10-21T02:10:14Z2021-10-21T02:10:14Z2021-10-202005info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleChemical Speciation & Bioavailability, v. 17, n. 3, p. 109-116, 2005.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135457https://doi.org/10.3184/095422905782774865enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-10-21T02:10:25Zoai:www.alice.cnptia.embrapa.br:doc/1135457Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-10-21T02:10:25falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-10-21T02:10:25Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. |
title |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. |
spellingShingle |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. PEREZ, D. V. SEC-HPLC Geochem-PC |
title_short |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. |
title_full |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. |
title_fullStr |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. |
title_full_unstemmed |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. |
title_sort |
The effect of two kinds of eluents on the speciation of Al, Mn, and Fe by size-exclusion chromatography of some Brazilian soil solutions. |
author |
PEREZ, D. V. |
author_facet |
PEREZ, D. V. CAMPOS, R. C. de |
author_role |
author |
author2 |
CAMPOS, R. C. de |
author2_role |
author |
dc.contributor.none.fl_str_mv |
DANIEL VIDAL PEREZ, CNPS; REINALDO C. DE CAMPOS, PUC-RIO. |
dc.contributor.author.fl_str_mv |
PEREZ, D. V. CAMPOS, R. C. de |
dc.subject.por.fl_str_mv |
SEC-HPLC Geochem-PC |
topic |
SEC-HPLC Geochem-PC |
description |
Size-exclusion chromatography is a powerful tool for distinguishing dissolved organic matter in relation to its molecular size. However, problems may arise in the choice of the most appropriate eluent composition, regarding the gel used. In the present work, soil solution samples from four Brazilian topsoils were analyzed by high-performance liquid chromatography (HPLC) using a size-exclusion polymer-based column (Superdex 75 HR 10/30, Pharmacia). Water and 0.01 mol L-1 NaCl were investigated as mobile phases. The analysis of the UV peak distribution as well as their corresponding Fe, Mn, and Al concentrations suggested that the salt solution should be preferred since it minimizes unwanted interactions between the sample and the support material. Fe, Mn, and Al speciation calculated by the Geochem-PC program involving the Mattigod and Sposito Mixed Model was compared with the Fe, Mn, and Al-organic complex concentrations determined by SEC-HPLC/GFAAS. No correlation was found for Mn and Fe. For Al a significant non-linear correlation was observed. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 2021-10-21T02:10:14Z 2021-10-21T02:10:14Z 2021-10-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Chemical Speciation & Bioavailability, v. 17, n. 3, p. 109-116, 2005. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135457 https://doi.org/10.3184/095422905782774865 |
identifier_str_mv |
Chemical Speciation & Bioavailability, v. 17, n. 3, p. 109-116, 2005. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1135457 https://doi.org/10.3184/095422905782774865 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503510886187008 |