Genetic diversity analysis among pigeonpea genotypes adapted to South American regions based on microsatellite markers.

• Autor(a) principal: SOUSA, A. C. B.
• Data de Publicação: 2011
• Outros Autores: GODOY, R., SFORÇA, D. A., CAMPOS, T. de, ZUCCHI, M. I., JANK, L., SOUZA, A. P. de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902585
• topic: Inferência bayesiana; Bayesian inference; Sofware Structure; Simulación por computadora; Variación genética; Análisis estadístico; Frijoles; Guandul; Marcadores genéticos; Guandu; Cajanus cajan; Variação genética; Marcador genético; Polimorfismo genético; Feijão; Phaseolus vulgaris; Feijão de corda; Vigna unguiculata; Método estatístico; Modelo de simulação; Pigeon peas; Genetic variation; Genetic markers; Statistical analysis; Genetic polymorphism; Beans; Computer simulation

The pigeonpea [Cajanus cajan (L) Millspaugh] is one of the most important perennial legume crops utilized in the food, fodder, soil conservation, crop-livestock integrated systems, reclaiming of degraded pastures and symbiotic nitrogen fixation. Microsatellite markers were used to estimate the genetic diversity of 77 pigeonpea genotypes selected from the germplasm collections at Embrapa Cattle-Southeast and, to evaluate their transferability to Phaseolus vulgaris and Vigna unguiculata species. The number of alleles per locus ranged from 2 to12, with an average of 5.1 alleles. The PIC values ranged from 0.11 to 0.80 (average 0.49) and the D values from 0.23 to 0.91 (average 0.58). The averages of observed and expected heterozygosity were 0.25 and 0.47, respectively, showing a deficit in heterozygosity. A model-based Bayesian approach implemented in the software STRUCTURE was used to assign genotypes into clusters. A dendrogram was constructed based on the modified Roger's genetic distances using a neighbor-joining method (NJ). A total of four clusters were assembled by STRUCTURE and a strong tendency of correspondence between the Bayesian clusters in the NJ tree was observed. The genetic distance ranged from 0.09 to 0.62 (average 0.37), showing a low genetic diversity in the pigeonpea genotypes. Transferability of pigeonpea-specific microsatellites revealed a cross-amplification and the presence of polymorphic alleles in P. vulgaris and V. unguiculata.