Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
| Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108670 |
Resumo: | The present study aimed to investigate the effect of quercetin as an alternative antioxidant tocysteamine onin vitromaturation. Oocytes were collected from goat ovaries, destined forin vitromaturation and distributed into three groups: CIS group, oocytes were immersed inMIV base medium; in Groups Q4 and Q8, oocytes were immersed in the medium of the CISgroup, adding 4μMor8μM of quercetin, respectively, and cultured for 24 h at 38.5°C with5% CO2. The CIS and Q4 groups presented the same percentage of expanded cumulus cells,but the per cent in the Q8 group was significantly lower than that of the other groups(P<0.05). The oocyte retraction rate in the Q8 group was higher (P<0.05) than in the CISand Q4 groups. Treatment with 8μM of quercetin presented a lower proportion of expandedoocytes than the CIS group and 4μM of quercetin (P<0.05). The percentage of MII oocyteswas higher in the Q4 group than in the CIS group (P<0.05), but the percentages in the CISand Q8 groups were similar. The rate of apoptosis was higher in the CIS group than in theother groups (P<0.05). In addition, oocytes matured with 4μM quercetin showed highermitochondrial activity than matured oocytes in the CIS and Q8 groups (P<0.05). Inconclusion, 4μM of quercetin can be used as an alternative to cysteamine in thein vitromaturation of goat oocytes |
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Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes.CaprinoGoatsDairy goatsThe present study aimed to investigate the effect of quercetin as an alternative antioxidant tocysteamine onin vitromaturation. Oocytes were collected from goat ovaries, destined forin vitromaturation and distributed into three groups: CIS group, oocytes were immersed inMIV base medium; in Groups Q4 and Q8, oocytes were immersed in the medium of the CISgroup, adding 4μMor8μM of quercetin, respectively, and cultured for 24 h at 38.5°C with5% CO2. The CIS and Q4 groups presented the same percentage of expanded cumulus cells,but the per cent in the Q8 group was significantly lower than that of the other groups(P<0.05). The oocyte retraction rate in the Q8 group was higher (P<0.05) than in the CISand Q4 groups. Treatment with 8μM of quercetin presented a lower proportion of expandedoocytes than the CIS group and 4μM of quercetin (P<0.05). The percentage of MII oocyteswas higher in the Q4 group than in the CIS group (P<0.05), but the percentages in the CISand Q8 groups were similar. The rate of apoptosis was higher in the CIS group than in theother groups (P<0.05). In addition, oocytes matured with 4μM quercetin showed highermitochondrial activity than matured oocytes in the CIS and Q8 groups (P<0.05). Inconclusion, 4μM of quercetin can be used as an alternative to cysteamine in thein vitromaturation of goat oocytesDANIEL MAIA NOGUEIRA, CPATSA.SILVA, A. A. A.SILVA, M. N. P.FIGUEIREDO, L. B. F.GONÇALVES, J. D.SILVA, M. J. S.LOIOLA, M. L. G.BASTOS, B. D. M.OLIVEIRA, R. A.RIBEIRO, L. G. M.BARBERINO, R. S.GOUVEIA, B. B.MONTE, A. P. O.NOGUEIRA, D. M.CORDEIRO, M. F.MATOS, M. H. T.LOPES JÚNIOR, E. S.2019-05-04T00:47:58Z2019-05-04T00:47:58Z2019-05-0320182020-01-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleZygote, v. 26, n. 6, p. 465-470, dez. 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/110867010.1017/S0967199418000485enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-05-04T00:48:07Zoai:www.alice.cnptia.embrapa.br:doc/1108670Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-05-04T00:48:07Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
| dc.title.none.fl_str_mv |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. |
| title |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. |
| spellingShingle |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. SILVA, A. A. A. Caprino Goats Dairy goats |
| title_short |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. |
| title_full |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. |
| title_fullStr |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. |
| title_full_unstemmed |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. |
| title_sort |
Quercetin influences in vitro maturation, apoptosis and metabolically active mitochondria of goat oocytes. |
| author |
SILVA, A. A. A. |
| author_facet |
SILVA, A. A. A. SILVA, M. N. P. FIGUEIREDO, L. B. F. GONÇALVES, J. D. SILVA, M. J. S. LOIOLA, M. L. G. BASTOS, B. D. M. OLIVEIRA, R. A. RIBEIRO, L. G. M. BARBERINO, R. S. GOUVEIA, B. B. MONTE, A. P. O. NOGUEIRA, D. M. CORDEIRO, M. F. MATOS, M. H. T. LOPES JÚNIOR, E. S. |
| author_role |
author |
| author2 |
SILVA, M. N. P. FIGUEIREDO, L. B. F. GONÇALVES, J. D. SILVA, M. J. S. LOIOLA, M. L. G. BASTOS, B. D. M. OLIVEIRA, R. A. RIBEIRO, L. G. M. BARBERINO, R. S. GOUVEIA, B. B. MONTE, A. P. O. NOGUEIRA, D. M. CORDEIRO, M. F. MATOS, M. H. T. LOPES JÚNIOR, E. S. |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
DANIEL MAIA NOGUEIRA, CPATSA. |
| dc.contributor.author.fl_str_mv |
SILVA, A. A. A. SILVA, M. N. P. FIGUEIREDO, L. B. F. GONÇALVES, J. D. SILVA, M. J. S. LOIOLA, M. L. G. BASTOS, B. D. M. OLIVEIRA, R. A. RIBEIRO, L. G. M. BARBERINO, R. S. GOUVEIA, B. B. MONTE, A. P. O. NOGUEIRA, D. M. CORDEIRO, M. F. MATOS, M. H. T. LOPES JÚNIOR, E. S. |
| dc.subject.por.fl_str_mv |
Caprino Goats Dairy goats |
| topic |
Caprino Goats Dairy goats |
| description |
The present study aimed to investigate the effect of quercetin as an alternative antioxidant tocysteamine onin vitromaturation. Oocytes were collected from goat ovaries, destined forin vitromaturation and distributed into three groups: CIS group, oocytes were immersed inMIV base medium; in Groups Q4 and Q8, oocytes were immersed in the medium of the CISgroup, adding 4μMor8μM of quercetin, respectively, and cultured for 24 h at 38.5°C with5% CO2. The CIS and Q4 groups presented the same percentage of expanded cumulus cells,but the per cent in the Q8 group was significantly lower than that of the other groups(P<0.05). The oocyte retraction rate in the Q8 group was higher (P<0.05) than in the CISand Q4 groups. Treatment with 8μM of quercetin presented a lower proportion of expandedoocytes than the CIS group and 4μM of quercetin (P<0.05). The percentage of MII oocyteswas higher in the Q4 group than in the CIS group (P<0.05), but the percentages in the CISand Q8 groups were similar. The rate of apoptosis was higher in the CIS group than in theother groups (P<0.05). In addition, oocytes matured with 4μM quercetin showed highermitochondrial activity than matured oocytes in the CIS and Q8 groups (P<0.05). Inconclusion, 4μM of quercetin can be used as an alternative to cysteamine in thein vitromaturation of goat oocytes |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2019-05-04T00:47:58Z 2019-05-04T00:47:58Z 2019-05-03 2020-01-20T11:11:11Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
Zygote, v. 26, n. 6, p. 465-470, dez. 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108670 10.1017/S0967199418000485 |
| identifier_str_mv |
Zygote, v. 26, n. 6, p. 465-470, dez. 2018. 10.1017/S0967199418000485 |
| url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1108670 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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cg-riaa@embrapa.br |
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