Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil

Detalhes bibliográficos
Autor(a) principal: Lopes, Luciana Florêncio Vilaça
Data de Publicação: 2019
Outros Autores: Silva, Elizabete Cristina da, Moraes, Anidene Christina Alves de, Silva, Elizabete Rodrigues da, Santoro, Kleber Régis, Batista, Ângela Maria Vieira, Barbosa, Severino Benone Paes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34171
Resumo: The objective of this study was to detect Mycoplasma agalactiae (Ma) and the Mycoplasma mycoides cluster (Mmcluster) in 373 goat milk samples of different breeds from herds located in Pernambuco and Paraíba states, as well as to evaluate somatic cell count (SCC) and milk composition from positive animals. For this, DNA extraction from milk samples was carried out, followed by generic and species-specific amplification by Polymerase Chain Reaction (PCR). Milk constituents were determined by medium infrared spectrometry and SCC by flow cytometry. Analyses of variance and tests of comparison of means verified the effects of positivity on the evaluated characteristics. The frequencies for Ma and Mmcluster were 43.21 and 5.70%, respectively. In all genetic groups, Ma was detected in all positive samples, whereas Mmcluster was only observed in samples from Moxotó, Parda Sertaneja, and that without a defined racial pattern. Statistical difference was observed (p < 0.05) between mean values of protein, casein and SCC in positive and negative Ma samples. In terms of Mmcluster there was only a statistically significant difference in the SCC parameter. The detection of Mycoplasma in samples of goat milk suggests an introduction of infected animals into the evaluated herds, as well as possible contact with the etiological agents at fairs and exhibitions.
id UEL-11_bbdceadb8a979170e4ce776b251c680e
oai_identifier_str oai:ojs.pkp.sfu.ca:article/34171
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, BrazilMycoplasma agalactiae e Mycoplasma mycoides cluster em rebanhos caprinos nos estados de Pernambuco e Paraíba, BrasilContagious agalactiaeDairy goatsMycoplasmosis.Agalactia contagiosaCaprinos leiteirosMicoplasmose.The objective of this study was to detect Mycoplasma agalactiae (Ma) and the Mycoplasma mycoides cluster (Mmcluster) in 373 goat milk samples of different breeds from herds located in Pernambuco and Paraíba states, as well as to evaluate somatic cell count (SCC) and milk composition from positive animals. For this, DNA extraction from milk samples was carried out, followed by generic and species-specific amplification by Polymerase Chain Reaction (PCR). Milk constituents were determined by medium infrared spectrometry and SCC by flow cytometry. Analyses of variance and tests of comparison of means verified the effects of positivity on the evaluated characteristics. The frequencies for Ma and Mmcluster were 43.21 and 5.70%, respectively. In all genetic groups, Ma was detected in all positive samples, whereas Mmcluster was only observed in samples from Moxotó, Parda Sertaneja, and that without a defined racial pattern. Statistical difference was observed (p < 0.05) between mean values of protein, casein and SCC in positive and negative Ma samples. In terms of Mmcluster there was only a statistically significant difference in the SCC parameter. The detection of Mycoplasma in samples of goat milk suggests an introduction of infected animals into the evaluated herds, as well as possible contact with the etiological agents at fairs and exhibitions.O presente trabalho teve como objetivo detectar Mycoplasma agalactiae (Ma) e Mycoplasma mycoides cluster (Mmcluster) em 373 amostras de leite caprino de diferentes raças, pertencentes a rebanhos localizados nos estados de Pernambuco e Paraíba, bem como avaliar a composição e a contagem de células somáticas (CCS) de leite de animais positivos para aqueles agentes. Para isso realizou-se a extração de DNA de amostras de leite, seguida de amplificação genérica e espécie-específica por Reação em Cadeia da Polimerase (PCR). Os constituintes do leite foram determinados por espectrômetro de infravermelho médio e a CCS, por citometria de fluxo. As análises de variância e testes de comparação de médias verificaram os efeitos da positividade sobre as características avaliadas. As frequências para Ma e Mmclusterforam de 43,21 e 5,70%, respectivamente. Em todos os grupos genéticos foram detectadas amostras positivas para Ma, enquanto que para Mmcluster só foram observadas em animais das raças Moxotó, Parda Sertaneja e sem padrão racial definido. Observou-se diferença estatística (p < 0,05) entre as médias de proteína, caseína e CCS de amostras positivas e negativas para Ma. Para Mmcluster, verificou-se diferença estatística significativa apenas para o parâmetro CCS. A detecção de Mycoplasma em amostras de leite caprino sugere a introdução de animais infectados nos rebanhos avaliados, como também o possível contato com os agentes etiológicos em feiras e exposições.UEL2019-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3417110.5433/1679-0359.2019v40n5Supl1p2261Semina: Ciências Agrárias; Vol. 40 No. 5Supl1 (2019); 2261-2270Semina: Ciências Agrárias; v. 40 n. 5Supl1 (2019); 2261-22701679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34171/25847Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessLopes, Luciana Florêncio VilaçaSilva, Elizabete Cristina daMoraes, Anidene Christina Alves deSilva, Elizabete Rodrigues daSantoro, Kleber RégisBatista, Ângela Maria VieiraBarbosa, Severino Benone Paes2022-10-18T16:09:12Zoai:ojs.pkp.sfu.ca:article/34171Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-18T16:09:12Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
Mycoplasma agalactiae e Mycoplasma mycoides cluster em rebanhos caprinos nos estados de Pernambuco e Paraíba, Brasil
title Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
spellingShingle Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
Lopes, Luciana Florêncio Vilaça
Contagious agalactiae
Dairy goats
Mycoplasmosis.
Agalactia contagiosa
Caprinos leiteiros
Micoplasmose.
title_short Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
title_full Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
title_fullStr Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
title_full_unstemmed Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
title_sort Mycoplasma agalactiae and the Mycoplasma mycoides cluster in goat herds in the states of Pernambuco and Paraíba, Brazil
author Lopes, Luciana Florêncio Vilaça
author_facet Lopes, Luciana Florêncio Vilaça
Silva, Elizabete Cristina da
Moraes, Anidene Christina Alves de
Silva, Elizabete Rodrigues da
Santoro, Kleber Régis
Batista, Ângela Maria Vieira
Barbosa, Severino Benone Paes
author_role author
author2 Silva, Elizabete Cristina da
Moraes, Anidene Christina Alves de
Silva, Elizabete Rodrigues da
Santoro, Kleber Régis
Batista, Ângela Maria Vieira
Barbosa, Severino Benone Paes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lopes, Luciana Florêncio Vilaça
Silva, Elizabete Cristina da
Moraes, Anidene Christina Alves de
Silva, Elizabete Rodrigues da
Santoro, Kleber Régis
Batista, Ângela Maria Vieira
Barbosa, Severino Benone Paes
dc.subject.por.fl_str_mv Contagious agalactiae
Dairy goats
Mycoplasmosis.
Agalactia contagiosa
Caprinos leiteiros
Micoplasmose.
topic Contagious agalactiae
Dairy goats
Mycoplasmosis.
Agalactia contagiosa
Caprinos leiteiros
Micoplasmose.
description The objective of this study was to detect Mycoplasma agalactiae (Ma) and the Mycoplasma mycoides cluster (Mmcluster) in 373 goat milk samples of different breeds from herds located in Pernambuco and Paraíba states, as well as to evaluate somatic cell count (SCC) and milk composition from positive animals. For this, DNA extraction from milk samples was carried out, followed by generic and species-specific amplification by Polymerase Chain Reaction (PCR). Milk constituents were determined by medium infrared spectrometry and SCC by flow cytometry. Analyses of variance and tests of comparison of means verified the effects of positivity on the evaluated characteristics. The frequencies for Ma and Mmcluster were 43.21 and 5.70%, respectively. In all genetic groups, Ma was detected in all positive samples, whereas Mmcluster was only observed in samples from Moxotó, Parda Sertaneja, and that without a defined racial pattern. Statistical difference was observed (p < 0.05) between mean values of protein, casein and SCC in positive and negative Ma samples. In terms of Mmcluster there was only a statistically significant difference in the SCC parameter. The detection of Mycoplasma in samples of goat milk suggests an introduction of infected animals into the evaluated herds, as well as possible contact with the etiological agents at fairs and exhibitions.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34171
10.5433/1679-0359.2019v40n5Supl1p2261
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34171
identifier_str_mv 10.5433/1679-0359.2019v40n5Supl1p2261
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/34171/25847
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 40 No. 5Supl1 (2019); 2261-2270
Semina: Ciências Agrárias; v. 40 n. 5Supl1 (2019); 2261-2270
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306080413548544

Registros relacionados