Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).

Detalhes bibliográficos
Autor(a) principal: GUIMARÃES, D. A.
Data de Publicação: 2012
Outros Autores: GARCIA, S. C. G. de, FERREIRA, M. A. P., SILVA, S. do S. B. da, ALBUQUERQUE, N. I. de, LE PENDU, Y.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/950273
Resumo: The sustainability and production of collared peccary (Pecari tajacu) has been studied in the last few years; however, further information on its reproduction is necessary for breeding systems success. Understanding folliculogenesis aspects will contribute to effective reproductive biotechniques, which are useful in the preservation and production of wildlife. The aim of this study was to evaluate the ovarian folliculogenesis in collared peccary. Ovaries from six adult females of collared peccary were obtained through ovariectomy and analyzed. These were fixed in aqueous Bouin?s solution and sectioned into 7µm slices, stained with hematoxilin-eosin and analyzed by light microscopy. The number of pre-antral and antral follicles per ovary was estimated using the Fractionator Method. The follicles, oocytes and oocyte nuclei were measured using an ocular micrometer. Results showed that the length, width, thickness, weight, and the gross anatomy of the right and left ovaries were not significantly different. However, the mean number of corpora lutea was different between the phases of the estrous cycle (p<0.05), with the highest mean in the luteal phase. Primordial follicles were found in the cortex; the oocytes were enveloped by a single layer of flattened follicular cells. In the primary follicles, proliferation of the follicular cells gave rise to cuboidal cells (granulosa cells). The secondary follicle was characterized by two or more concentric layers of cuboidal cells (granulosa), beginning of antrum formation, and the presence of pellucid zone and theca cells. Antral follicles were characterized by a central cavity (antrum), the presence of cumulus oophorus and theca layers (interna and externa). In the right ovary, the values of the primordial and primary follicles were similar, but significantly different from the secondary ones (p<0.05). In the left ovary, significant differences were observed between all follicles in the follicular phase (p<0.05); the mean number of primordial and primary follicles was similar in the luteal phase. The mean number of pre-antral follicles and antral follicles in the follicular phase was higher in the left ovary (p<0.05). The mean number of antral follicles in the luteal phase was similar in both ovaries. We also found significant differences in mean diameter of preantral follicles, oocyte, granulosa layer and oocyte nucleus during the estrous cycle. In the antral follicles a significant difference was observed only in follicular diameter (p<0.05). The predominance of active primordial and primary follicles was found in both phases; otherwise the secondary follicles and antral follicles showed a high degree of degeneration. The results obtained in the present work will strengthen the development of biotechnology programs to improve the productive potential and conservation of the collared peccary
id EMBR_22ac9a54f9b89dda816e63057258f4f7
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/950273
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).Pecari tajacuMorfologia animalAnatomia animalOvarioThe sustainability and production of collared peccary (Pecari tajacu) has been studied in the last few years; however, further information on its reproduction is necessary for breeding systems success. Understanding folliculogenesis aspects will contribute to effective reproductive biotechniques, which are useful in the preservation and production of wildlife. The aim of this study was to evaluate the ovarian folliculogenesis in collared peccary. Ovaries from six adult females of collared peccary were obtained through ovariectomy and analyzed. These were fixed in aqueous Bouin?s solution and sectioned into 7µm slices, stained with hematoxilin-eosin and analyzed by light microscopy. The number of pre-antral and antral follicles per ovary was estimated using the Fractionator Method. The follicles, oocytes and oocyte nuclei were measured using an ocular micrometer. Results showed that the length, width, thickness, weight, and the gross anatomy of the right and left ovaries were not significantly different. However, the mean number of corpora lutea was different between the phases of the estrous cycle (p<0.05), with the highest mean in the luteal phase. Primordial follicles were found in the cortex; the oocytes were enveloped by a single layer of flattened follicular cells. In the primary follicles, proliferation of the follicular cells gave rise to cuboidal cells (granulosa cells). The secondary follicle was characterized by two or more concentric layers of cuboidal cells (granulosa), beginning of antrum formation, and the presence of pellucid zone and theca cells. Antral follicles were characterized by a central cavity (antrum), the presence of cumulus oophorus and theca layers (interna and externa). In the right ovary, the values of the primordial and primary follicles were similar, but significantly different from the secondary ones (p<0.05). In the left ovary, significant differences were observed between all follicles in the follicular phase (p<0.05); the mean number of primordial and primary follicles was similar in the luteal phase. The mean number of pre-antral follicles and antral follicles in the follicular phase was higher in the left ovary (p<0.05). The mean number of antral follicles in the luteal phase was similar in both ovaries. We also found significant differences in mean diameter of preantral follicles, oocyte, granulosa layer and oocyte nucleus during the estrous cycle. In the antral follicles a significant difference was observed only in follicular diameter (p<0.05). The predominance of active primordial and primary follicles was found in both phases; otherwise the secondary follicles and antral follicles showed a high degree of degeneration. The results obtained in the present work will strengthen the development of biotechnology programs to improve the productive potential and conservation of the collared peccaryDIVA ANELIA GUIMARÃES, UFPA; SYLVIA CRISTINA GARCIA DE GARCIA, UFPA; MARIA AUXILIADORA PANTOJA FERREIRA, UFPA; SULEIMA DO SOCORRO BASTOS DA SILVA, UFPA; NATALIA INAGAKI DE ALBUQUERQUE, CPATU; YVONNICK LE PENDU, UNIVERSIDADE DE COSTA RICA.GUIMARÃES, D. A.GARCIA, S. C. G. deFERREIRA, M. A. P.SILVA, S. do S. B. daALBUQUERQUE, N. I. deLE PENDU, Y.2013-02-20T11:11:11Z2013-02-20T11:11:11Z2013-02-2020122016-09-14T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista de Biologia Tropical, v. 60, n. 1, p. 437-445, Mar. 2012.0034-7744http://www.alice.cnptia.embrapa.br/alice/handle/doc/950273enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:05:03Zoai:www.alice.cnptia.embrapa.br:doc/950273Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:05:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:05:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
title Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
spellingShingle Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
GUIMARÃES, D. A.
Pecari tajacu
Morfologia animal
Anatomia animal
Ovario
title_short Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
title_full Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
title_fullStr Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
title_full_unstemmed Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
title_sort Ovarian folliculogenesis in collared peccary, Pecari tajacu (Artiodactyla: Tayassuidae).
author GUIMARÃES, D. A.
author_facet GUIMARÃES, D. A.
GARCIA, S. C. G. de
FERREIRA, M. A. P.
SILVA, S. do S. B. da
ALBUQUERQUE, N. I. de
LE PENDU, Y.
author_role author
author2 GARCIA, S. C. G. de
FERREIRA, M. A. P.
SILVA, S. do S. B. da
ALBUQUERQUE, N. I. de
LE PENDU, Y.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv DIVA ANELIA GUIMARÃES, UFPA; SYLVIA CRISTINA GARCIA DE GARCIA, UFPA; MARIA AUXILIADORA PANTOJA FERREIRA, UFPA; SULEIMA DO SOCORRO BASTOS DA SILVA, UFPA; NATALIA INAGAKI DE ALBUQUERQUE, CPATU; YVONNICK LE PENDU, UNIVERSIDADE DE COSTA RICA.
dc.contributor.author.fl_str_mv GUIMARÃES, D. A.
GARCIA, S. C. G. de
FERREIRA, M. A. P.
SILVA, S. do S. B. da
ALBUQUERQUE, N. I. de
LE PENDU, Y.
dc.subject.por.fl_str_mv Pecari tajacu
Morfologia animal
Anatomia animal
Ovario
topic Pecari tajacu
Morfologia animal
Anatomia animal
Ovario
description The sustainability and production of collared peccary (Pecari tajacu) has been studied in the last few years; however, further information on its reproduction is necessary for breeding systems success. Understanding folliculogenesis aspects will contribute to effective reproductive biotechniques, which are useful in the preservation and production of wildlife. The aim of this study was to evaluate the ovarian folliculogenesis in collared peccary. Ovaries from six adult females of collared peccary were obtained through ovariectomy and analyzed. These were fixed in aqueous Bouin?s solution and sectioned into 7µm slices, stained with hematoxilin-eosin and analyzed by light microscopy. The number of pre-antral and antral follicles per ovary was estimated using the Fractionator Method. The follicles, oocytes and oocyte nuclei were measured using an ocular micrometer. Results showed that the length, width, thickness, weight, and the gross anatomy of the right and left ovaries were not significantly different. However, the mean number of corpora lutea was different between the phases of the estrous cycle (p<0.05), with the highest mean in the luteal phase. Primordial follicles were found in the cortex; the oocytes were enveloped by a single layer of flattened follicular cells. In the primary follicles, proliferation of the follicular cells gave rise to cuboidal cells (granulosa cells). The secondary follicle was characterized by two or more concentric layers of cuboidal cells (granulosa), beginning of antrum formation, and the presence of pellucid zone and theca cells. Antral follicles were characterized by a central cavity (antrum), the presence of cumulus oophorus and theca layers (interna and externa). In the right ovary, the values of the primordial and primary follicles were similar, but significantly different from the secondary ones (p<0.05). In the left ovary, significant differences were observed between all follicles in the follicular phase (p<0.05); the mean number of primordial and primary follicles was similar in the luteal phase. The mean number of pre-antral follicles and antral follicles in the follicular phase was higher in the left ovary (p<0.05). The mean number of antral follicles in the luteal phase was similar in both ovaries. We also found significant differences in mean diameter of preantral follicles, oocyte, granulosa layer and oocyte nucleus during the estrous cycle. In the antral follicles a significant difference was observed only in follicular diameter (p<0.05). The predominance of active primordial and primary follicles was found in both phases; otherwise the secondary follicles and antral follicles showed a high degree of degeneration. The results obtained in the present work will strengthen the development of biotechnology programs to improve the productive potential and conservation of the collared peccary
publishDate 2012
dc.date.none.fl_str_mv 2012
2013-02-20T11:11:11Z
2013-02-20T11:11:11Z
2013-02-20
2016-09-14T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista de Biologia Tropical, v. 60, n. 1, p. 437-445, Mar. 2012.
0034-7744
http://www.alice.cnptia.embrapa.br/alice/handle/doc/950273
identifier_str_mv Revista de Biologia Tropical, v. 60, n. 1, p. 437-445, Mar. 2012.
0034-7744
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/950273
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503375758295040