Differential expression of genes in follicular cells of swines.
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526 https://doi.org/10.1590/S1516-35982010000500012 |
Resumo: | The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines. |
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Differential expression of genes in follicular cells of swines.Genes candidatosProdução de suínosPCR quantitativo em tempo realTamanho de leitegadaOvulaçãoThe main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.CRISTIANA LIBARDI MIRANDA FURTADO, UFV; PRISCILA VENDRAMINI SILVA, UFV; MARTA FONSECA MARTINS GUIMARAES, CNPGL; NICOLA VERGARA LOPES SERÃO, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE ELIZA FACIONI GUIMARÃES, UFV.FURTADO, C. L. M.SILVA, P. V.GUIMARAES, M. F. M.SERÃO, N. V. L.GUIMARÃES, J. D.GUIMARÃES, S. E. F.2023-01-23T19:02:08Z2023-01-23T19:02:08Z2011-03-182010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Zootecnia, v. 39,n. 5, p. p.1023-1028, 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526https://doi.org/10.1590/S1516-35982010000500012enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-01-23T19:02:08Zoai:www.alice.cnptia.embrapa.br:doc/881526Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-01-23T19:02:08falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-01-23T19:02:08Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Differential expression of genes in follicular cells of swines. |
title |
Differential expression of genes in follicular cells of swines. |
spellingShingle |
Differential expression of genes in follicular cells of swines. FURTADO, C. L. M. Genes candidatos Produção de suínos PCR quantitativo em tempo real Tamanho de leitegada Ovulação |
title_short |
Differential expression of genes in follicular cells of swines. |
title_full |
Differential expression of genes in follicular cells of swines. |
title_fullStr |
Differential expression of genes in follicular cells of swines. |
title_full_unstemmed |
Differential expression of genes in follicular cells of swines. |
title_sort |
Differential expression of genes in follicular cells of swines. |
author |
FURTADO, C. L. M. |
author_facet |
FURTADO, C. L. M. SILVA, P. V. GUIMARAES, M. F. M. SERÃO, N. V. L. GUIMARÃES, J. D. GUIMARÃES, S. E. F. |
author_role |
author |
author2 |
SILVA, P. V. GUIMARAES, M. F. M. SERÃO, N. V. L. GUIMARÃES, J. D. GUIMARÃES, S. E. F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
CRISTIANA LIBARDI MIRANDA FURTADO, UFV; PRISCILA VENDRAMINI SILVA, UFV; MARTA FONSECA MARTINS GUIMARAES, CNPGL; NICOLA VERGARA LOPES SERÃO, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE ELIZA FACIONI GUIMARÃES, UFV. |
dc.contributor.author.fl_str_mv |
FURTADO, C. L. M. SILVA, P. V. GUIMARAES, M. F. M. SERÃO, N. V. L. GUIMARÃES, J. D. GUIMARÃES, S. E. F. |
dc.subject.por.fl_str_mv |
Genes candidatos Produção de suínos PCR quantitativo em tempo real Tamanho de leitegada Ovulação |
topic |
Genes candidatos Produção de suínos PCR quantitativo em tempo real Tamanho de leitegada Ovulação |
description |
The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2011-03-18 2023-01-23T19:02:08Z 2023-01-23T19:02:08Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Zootecnia, v. 39,n. 5, p. p.1023-1028, 2010. http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526 https://doi.org/10.1590/S1516-35982010000500012 |
identifier_str_mv |
Revista Brasileira de Zootecnia, v. 39,n. 5, p. p.1023-1028, 2010. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526 https://doi.org/10.1590/S1516-35982010000500012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503538263457792 |