Differential expression of genes in follicular cells of swines.

Detalhes bibliográficos
Autor(a) principal: FURTADO, C. L. M.
Data de Publicação: 2010
Outros Autores: SILVA, P. V., GUIMARAES, M. F. M., SERÃO, N. V. L., GUIMARÃES, J. D., GUIMARÃES, S. E. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526
https://doi.org/10.1590/S1516-35982010000500012
Resumo: The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.
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spelling Differential expression of genes in follicular cells of swines.Genes candidatosProdução de suínosPCR quantitativo em tempo realTamanho de leitegadaOvulaçãoThe main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.CRISTIANA LIBARDI MIRANDA FURTADO, UFV; PRISCILA VENDRAMINI SILVA, UFV; MARTA FONSECA MARTINS GUIMARAES, CNPGL; NICOLA VERGARA LOPES SERÃO, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE ELIZA FACIONI GUIMARÃES, UFV.FURTADO, C. L. M.SILVA, P. V.GUIMARAES, M. F. M.SERÃO, N. V. L.GUIMARÃES, J. D.GUIMARÃES, S. E. F.2023-01-23T19:02:08Z2023-01-23T19:02:08Z2011-03-182010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Zootecnia, v. 39,n. 5, p. p.1023-1028, 2010.http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526https://doi.org/10.1590/S1516-35982010000500012enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-01-23T19:02:08Zoai:www.alice.cnptia.embrapa.br:doc/881526Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-01-23T19:02:08falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-01-23T19:02:08Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Differential expression of genes in follicular cells of swines.
title Differential expression of genes in follicular cells of swines.
spellingShingle Differential expression of genes in follicular cells of swines.
FURTADO, C. L. M.
Genes candidatos
Produção de suínos
PCR quantitativo em tempo real
Tamanho de leitegada
Ovulação
title_short Differential expression of genes in follicular cells of swines.
title_full Differential expression of genes in follicular cells of swines.
title_fullStr Differential expression of genes in follicular cells of swines.
title_full_unstemmed Differential expression of genes in follicular cells of swines.
title_sort Differential expression of genes in follicular cells of swines.
author FURTADO, C. L. M.
author_facet FURTADO, C. L. M.
SILVA, P. V.
GUIMARAES, M. F. M.
SERÃO, N. V. L.
GUIMARÃES, J. D.
GUIMARÃES, S. E. F.
author_role author
author2 SILVA, P. V.
GUIMARAES, M. F. M.
SERÃO, N. V. L.
GUIMARÃES, J. D.
GUIMARÃES, S. E. F.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv CRISTIANA LIBARDI MIRANDA FURTADO, UFV; PRISCILA VENDRAMINI SILVA, UFV; MARTA FONSECA MARTINS GUIMARAES, CNPGL; NICOLA VERGARA LOPES SERÃO, UFV; JOSÉ DOMINGOS GUIMARÃES, UFV; SIMONE ELIZA FACIONI GUIMARÃES, UFV.
dc.contributor.author.fl_str_mv FURTADO, C. L. M.
SILVA, P. V.
GUIMARAES, M. F. M.
SERÃO, N. V. L.
GUIMARÃES, J. D.
GUIMARÃES, S. E. F.
dc.subject.por.fl_str_mv Genes candidatos
Produção de suínos
PCR quantitativo em tempo real
Tamanho de leitegada
Ovulação
topic Genes candidatos
Produção de suínos
PCR quantitativo em tempo real
Tamanho de leitegada
Ovulação
description The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.
publishDate 2010
dc.date.none.fl_str_mv 2010
2011-03-18
2023-01-23T19:02:08Z
2023-01-23T19:02:08Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Zootecnia, v. 39,n. 5, p. p.1023-1028, 2010.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526
https://doi.org/10.1590/S1516-35982010000500012
identifier_str_mv Revista Brasileira de Zootecnia, v. 39,n. 5, p. p.1023-1028, 2010.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/881526
https://doi.org/10.1590/S1516-35982010000500012
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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