Differential expression of genes in follicular cells of swines

Detalhes bibliográficos
Autor(a) principal: Miranda-Furtado,Cristiana Libardi
Data de Publicação: 2010
Outros Autores: Silva,Priscila Vendramini, Guimarães,Marta Fonseca Martins, Serão,Nicola Vergara Lopes, Guimarães,José Domingos, Guimarães,Simone Eliza Facioni
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012
Resumo: The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.
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spelling Differential expression of genes in follicular cells of swinescandidate genelitter sizeovulation ratepig productionquantitative real-time PCRThe main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.Sociedade Brasileira de Zootecnia2010-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012Revista Brasileira de Zootecnia v.39 n.5 2010reponame:Revista Brasileira de Zootecnia (Online)instname:Sociedade Brasileira de Zootecnia (SBZ)instacron:SBZ10.1590/S1516-35982010000500012info:eu-repo/semantics/openAccessMiranda-Furtado,Cristiana LibardiSilva,Priscila VendraminiGuimarães,Marta Fonseca MartinsSerão,Nicola Vergara LopesGuimarães,José DomingosGuimarães,Simone Eliza Facionieng2010-08-31T00:00:00Zoai:scielo:S1516-35982010000500012Revistahttps://www.rbz.org.br/pt-br/https://old.scielo.br/oai/scielo-oai.php||bz@sbz.org.br|| secretariarbz@sbz.org.br1806-92901516-3598opendoar:2010-08-31T00:00Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)false
dc.title.none.fl_str_mv Differential expression of genes in follicular cells of swines
title Differential expression of genes in follicular cells of swines
spellingShingle Differential expression of genes in follicular cells of swines
Miranda-Furtado,Cristiana Libardi
candidate gene
litter size
ovulation rate
pig production
quantitative real-time PCR
title_short Differential expression of genes in follicular cells of swines
title_full Differential expression of genes in follicular cells of swines
title_fullStr Differential expression of genes in follicular cells of swines
title_full_unstemmed Differential expression of genes in follicular cells of swines
title_sort Differential expression of genes in follicular cells of swines
author Miranda-Furtado,Cristiana Libardi
author_facet Miranda-Furtado,Cristiana Libardi
Silva,Priscila Vendramini
Guimarães,Marta Fonseca Martins
Serão,Nicola Vergara Lopes
Guimarães,José Domingos
Guimarães,Simone Eliza Facioni
author_role author
author2 Silva,Priscila Vendramini
Guimarães,Marta Fonseca Martins
Serão,Nicola Vergara Lopes
Guimarães,José Domingos
Guimarães,Simone Eliza Facioni
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Miranda-Furtado,Cristiana Libardi
Silva,Priscila Vendramini
Guimarães,Marta Fonseca Martins
Serão,Nicola Vergara Lopes
Guimarães,José Domingos
Guimarães,Simone Eliza Facioni
dc.subject.por.fl_str_mv candidate gene
litter size
ovulation rate
pig production
quantitative real-time PCR
topic candidate gene
litter size
ovulation rate
pig production
quantitative real-time PCR
description The main purpose of the present study was to identify for candidate genes related to ovulation in swines. To do so, it was investigated in ovarian follicular cells through quantitative real-time PCR the differential expression of the following genes: steroidogenic acute regulator (STAR), GATA-binding protein 4 (GATA), prostaglandin F2α (PGF2α), progesterone receptor (P4R), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (CYP19). These genes encode hormone receptors (FSHR and P4R), hormone (PGF2α), steroidogenic proteins (STAR and CYP19) and transcription factor (GATA). Folicular cells were collected from sows with high and low number of piglets/litters during the follicular phase of the estrus cycle. There was difference in transcript abundance among low and high prolific sows for the STAR, GATA, PGF2α, P4R and CYP19 genes. For the FSHR gene, the fold change was not considered to be significantly different. Because in the present study only the transcript level of the above mentioned genes was analyzed, no inference can be made regarded to protein translation or activity. Therefore, gene sequence trials and other functional studies will be necessary to complement the present results, allowing a better understanding on biological complexity of these genes and their use as markers for prolificity in swines.
publishDate 2010
dc.date.none.fl_str_mv 2010-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000500012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-35982010000500012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv Revista Brasileira de Zootecnia v.39 n.5 2010
reponame:Revista Brasileira de Zootecnia (Online)
instname:Sociedade Brasileira de Zootecnia (SBZ)
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institution SBZ
reponame_str Revista Brasileira de Zootecnia (Online)
collection Revista Brasileira de Zootecnia (Online)
repository.name.fl_str_mv Revista Brasileira de Zootecnia (Online) - Sociedade Brasileira de Zootecnia (SBZ)
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