Reproductive barriers in cassava: factors and implications for genetic improvement.

Detalhes bibliográficos
Autor(a) principal: SOUSA, M. B. e
Data de Publicação: 2021
Outros Autores: ANDRADE, L. R. B. de, SOUZA, E. H. de, ALVES, A. A. C., OLIVEIRA, E. J. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137018
Resumo: Cassava breeding is hampered by high flower abortion rates that prevent efficient recombination among promising clones. To better understand the factors causing flower abortion and propose strategies to overcome them, we 1) analyzed the reproductive barriers to intraspecific crossing, 2) evaluated pollen-pistil interactions to maximize hand pollination efficiency, and 3) identified the population structure of elite parental clones. From 2016 to 2018, the abortion and fertilization rates of 5,748 hand crossings involving 91 parents and 157 progenies were estimated. We used 16,300 single nucleotide polymorphism markers to study the parents' population structure via discriminant analysis of principal components, and three clusters were identified. To test for male and female effects, we used a mixed model in which the environment (month and year) was fixed, while female and male (nested to female) were random effects. Regardless of the population structure, significant parental effects were identified for abortion and fertilization rates, suggesting the existence of reproductive barriers among certain cassava clones. Matching ability between cassava parents was significant for pollen grains that adhered to the stigma surface, germinated pollen grains, and the number of fertilized ovules. Non-additive genetic effects were important to the inheritance of these traits. Pollen viability and pollen-pistil interactions in cross- and self-pollination were also investigated to characterize pollen-stigma compatibility. Various events related to pollen tube growth dynamics indicated fertilization abnormalities. These abnormalities included the reticulated deposition of callose in the pollen tube, pollen tube growth cessation in a specific region of the stylet, and low pollen grain germination rate. Generally, pollen viability and stigma receptivity varied depending on the clone and flowering stage and were lost during flowering. This study provides novel insights into cassava reproduction that can assist in practical crossing and maximize the recombination of contrasting clones.
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spelling Reproductive barriers in cassava: factors and implications for genetic improvement.MandiocaCassava breeding is hampered by high flower abortion rates that prevent efficient recombination among promising clones. To better understand the factors causing flower abortion and propose strategies to overcome them, we 1) analyzed the reproductive barriers to intraspecific crossing, 2) evaluated pollen-pistil interactions to maximize hand pollination efficiency, and 3) identified the population structure of elite parental clones. From 2016 to 2018, the abortion and fertilization rates of 5,748 hand crossings involving 91 parents and 157 progenies were estimated. We used 16,300 single nucleotide polymorphism markers to study the parents' population structure via discriminant analysis of principal components, and three clusters were identified. To test for male and female effects, we used a mixed model in which the environment (month and year) was fixed, while female and male (nested to female) were random effects. Regardless of the population structure, significant parental effects were identified for abortion and fertilization rates, suggesting the existence of reproductive barriers among certain cassava clones. Matching ability between cassava parents was significant for pollen grains that adhered to the stigma surface, germinated pollen grains, and the number of fertilized ovules. Non-additive genetic effects were important to the inheritance of these traits. Pollen viability and pollen-pistil interactions in cross- and self-pollination were also investigated to characterize pollen-stigma compatibility. Various events related to pollen tube growth dynamics indicated fertilization abnormalities. These abnormalities included the reticulated deposition of callose in the pollen tube, pollen tube growth cessation in a specific region of the stylet, and low pollen grain germination rate. Generally, pollen viability and stigma receptivity varied depending on the clone and flowering stage and were lost during flowering. This study provides novel insights into cassava reproduction that can assist in practical crossing and maximize the recombination of contrasting clones.MASSAINE BANDEIRA E SOUSA; LUCIANO ROGERIO BRAATZ DE ANDRADE; EVERTON HILO DE SOUZA, UFRB; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF.SOUSA, M. B. eANDRADE, L. R. B. deSOUZA, E. H. deALVES, A. A. C.OLIVEIRA, E. J. de2021-12-03T02:08:06Z2021-12-03T02:08:06Z2021-12-022021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, November, 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137018enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-12-03T02:08:15Zoai:www.alice.cnptia.embrapa.br:doc/1137018Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-12-03T02:08:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-12-03T02:08:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Reproductive barriers in cassava: factors and implications for genetic improvement.
title Reproductive barriers in cassava: factors and implications for genetic improvement.
spellingShingle Reproductive barriers in cassava: factors and implications for genetic improvement.
SOUSA, M. B. e
Mandioca
title_short Reproductive barriers in cassava: factors and implications for genetic improvement.
title_full Reproductive barriers in cassava: factors and implications for genetic improvement.
title_fullStr Reproductive barriers in cassava: factors and implications for genetic improvement.
title_full_unstemmed Reproductive barriers in cassava: factors and implications for genetic improvement.
title_sort Reproductive barriers in cassava: factors and implications for genetic improvement.
author SOUSA, M. B. e
author_facet SOUSA, M. B. e
ANDRADE, L. R. B. de
SOUZA, E. H. de
ALVES, A. A. C.
OLIVEIRA, E. J. de
author_role author
author2 ANDRADE, L. R. B. de
SOUZA, E. H. de
ALVES, A. A. C.
OLIVEIRA, E. J. de
author2_role author
author
author
author
dc.contributor.none.fl_str_mv MASSAINE BANDEIRA E SOUSA; LUCIANO ROGERIO BRAATZ DE ANDRADE; EVERTON HILO DE SOUZA, UFRB; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF.
dc.contributor.author.fl_str_mv SOUSA, M. B. e
ANDRADE, L. R. B. de
SOUZA, E. H. de
ALVES, A. A. C.
OLIVEIRA, E. J. de
dc.subject.por.fl_str_mv Mandioca
topic Mandioca
description Cassava breeding is hampered by high flower abortion rates that prevent efficient recombination among promising clones. To better understand the factors causing flower abortion and propose strategies to overcome them, we 1) analyzed the reproductive barriers to intraspecific crossing, 2) evaluated pollen-pistil interactions to maximize hand pollination efficiency, and 3) identified the population structure of elite parental clones. From 2016 to 2018, the abortion and fertilization rates of 5,748 hand crossings involving 91 parents and 157 progenies were estimated. We used 16,300 single nucleotide polymorphism markers to study the parents' population structure via discriminant analysis of principal components, and three clusters were identified. To test for male and female effects, we used a mixed model in which the environment (month and year) was fixed, while female and male (nested to female) were random effects. Regardless of the population structure, significant parental effects were identified for abortion and fertilization rates, suggesting the existence of reproductive barriers among certain cassava clones. Matching ability between cassava parents was significant for pollen grains that adhered to the stigma surface, germinated pollen grains, and the number of fertilized ovules. Non-additive genetic effects were important to the inheritance of these traits. Pollen viability and pollen-pistil interactions in cross- and self-pollination were also investigated to characterize pollen-stigma compatibility. Various events related to pollen tube growth dynamics indicated fertilization abnormalities. These abnormalities included the reticulated deposition of callose in the pollen tube, pollen tube growth cessation in a specific region of the stylet, and low pollen grain germination rate. Generally, pollen viability and stigma receptivity varied depending on the clone and flowering stage and were lost during flowering. This study provides novel insights into cassava reproduction that can assist in practical crossing and maximize the recombination of contrasting clones.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-03T02:08:06Z
2021-12-03T02:08:06Z
2021-12-02
2021
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dc.identifier.uri.fl_str_mv Plos One, November, 2021.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1137018
identifier_str_mv Plos One, November, 2021.
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dc.language.iso.fl_str_mv eng
language eng
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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