Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099951 |
Resumo: | The intergenerational transfer of plant defense compounds by aposematic insects is well documented, and since 2006, has been shown for Cry toxins. Cry toxins are proteins naturally produced by the soil bacterium Bacillus thuringiensis (Bt) and its genes have been expressed in plants to confer insect pest resistance. In this work we tested if non-aposematic larvae of a major maize pest, Spodoptera frugiperda, with resistance to Cry1F, could transfer Cry1F from a genetically engineered maize variety to their offspring. Resistant 10day-old larvae that fed on Cry1F Bt maize until pupation were sexed and pair-mated to produce eggs. Using ELISA we found that Cry1F was transferred to offspring (1.47–4.42 ng Cry1F/10 eggs), a toxin concentration about 28–83 times less than that detected in Cry1F Bt maize leaves. This occurred when only one or both sexes were exposed, and more was transferred when both parents were exposed, with transitory detection in the first five egg masses. This work is an unprecedented demonstration that a non-aposematic, but resistant, species can transfer Cry1F to their offspring when exposed to Bt host plant leaves as immatures. |
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Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda.Lagarta-do-cartuchoProteínaBacillus ThuringiensisPraga de PlantaControle BiológicoThe intergenerational transfer of plant defense compounds by aposematic insects is well documented, and since 2006, has been shown for Cry toxins. Cry toxins are proteins naturally produced by the soil bacterium Bacillus thuringiensis (Bt) and its genes have been expressed in plants to confer insect pest resistance. In this work we tested if non-aposematic larvae of a major maize pest, Spodoptera frugiperda, with resistance to Cry1F, could transfer Cry1F from a genetically engineered maize variety to their offspring. Resistant 10day-old larvae that fed on Cry1F Bt maize until pupation were sexed and pair-mated to produce eggs. Using ELISA we found that Cry1F was transferred to offspring (1.47–4.42 ng Cry1F/10 eggs), a toxin concentration about 28–83 times less than that detected in Cry1F Bt maize leaves. This occurred when only one or both sexes were exposed, and more was transferred when both parents were exposed, with transitory detection in the first five egg masses. This work is an unprecedented demonstration that a non-aposematic, but resistant, species can transfer Cry1F to their offspring when exposed to Bt host plant leaves as immatures.Camila S. F. Souza, Universidade Federal de Lavras; Luís C. P. Silveira, Universidade Federal de Lavras; DEBORA PIRES PAULA, Cenargen; David A. Andow, University of Minnesota; SIMONE MARTINS MENDES, CNPMS.SOUZA, C. S. F.SILVEIRA, L. C. P.PAULA, D. P.ANDOW, D. A.MENDES, S. M.2018-11-25T23:36:18Z2018-11-25T23:36:18Z2018-11-2320182018-11-25T23:36:18Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 13, n. 9, e0203791, Sept. 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/109995110.1371 /journal.pone. 0203791enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-11-25T23:36:24Zoai:www.alice.cnptia.embrapa.br:doc/1099951Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-11-25T23:36:24falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-11-25T23:36:24Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
title |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
spellingShingle |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. SOUZA, C. S. F. Lagarta-do-cartucho Proteína Bacillus Thuringiensis Praga de Planta Controle Biológico |
title_short |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
title_full |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
title_fullStr |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
title_full_unstemmed |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
title_sort |
Transfer of Cry1F from Bt maize to eggs of resistant Spodoptera frugiperda. |
author |
SOUZA, C. S. F. |
author_facet |
SOUZA, C. S. F. SILVEIRA, L. C. P. PAULA, D. P. ANDOW, D. A. MENDES, S. M. |
author_role |
author |
author2 |
SILVEIRA, L. C. P. PAULA, D. P. ANDOW, D. A. MENDES, S. M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Camila S. F. Souza, Universidade Federal de Lavras; Luís C. P. Silveira, Universidade Federal de Lavras; DEBORA PIRES PAULA, Cenargen; David A. Andow, University of Minnesota; SIMONE MARTINS MENDES, CNPMS. |
dc.contributor.author.fl_str_mv |
SOUZA, C. S. F. SILVEIRA, L. C. P. PAULA, D. P. ANDOW, D. A. MENDES, S. M. |
dc.subject.por.fl_str_mv |
Lagarta-do-cartucho Proteína Bacillus Thuringiensis Praga de Planta Controle Biológico |
topic |
Lagarta-do-cartucho Proteína Bacillus Thuringiensis Praga de Planta Controle Biológico |
description |
The intergenerational transfer of plant defense compounds by aposematic insects is well documented, and since 2006, has been shown for Cry toxins. Cry toxins are proteins naturally produced by the soil bacterium Bacillus thuringiensis (Bt) and its genes have been expressed in plants to confer insect pest resistance. In this work we tested if non-aposematic larvae of a major maize pest, Spodoptera frugiperda, with resistance to Cry1F, could transfer Cry1F from a genetically engineered maize variety to their offspring. Resistant 10day-old larvae that fed on Cry1F Bt maize until pupation were sexed and pair-mated to produce eggs. Using ELISA we found that Cry1F was transferred to offspring (1.47–4.42 ng Cry1F/10 eggs), a toxin concentration about 28–83 times less than that detected in Cry1F Bt maize leaves. This occurred when only one or both sexes were exposed, and more was transferred when both parents were exposed, with transitory detection in the first five egg masses. This work is an unprecedented demonstration that a non-aposematic, but resistant, species can transfer Cry1F to their offspring when exposed to Bt host plant leaves as immatures. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-25T23:36:18Z 2018-11-25T23:36:18Z 2018-11-23 2018 2018-11-25T23:36:18Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 13, n. 9, e0203791, Sept. 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099951 10.1371 /journal.pone. 0203791 |
identifier_str_mv |
Plos One, v. 13, n. 9, e0203791, Sept. 2018. 10.1371 /journal.pone. 0203791 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1099951 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503465670541312 |