Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae).
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123321 |
Resumo: | The genus Theobroma comprises several trees species native to the Amazon. Theobroma cacao L. plays a key economic role mainly in the chocolate industry. Both cultivated and wild forms are described within the genus. Variations in genome size and chromosome number have been used for prediction purposes including the frequency of interspecific hybridization or inference about evolutionary relationships. In this study, the nuclear DNA content, karyotype and genetic diversity using functional microsatellites (EST-SSR) of seven Theobroma species were characterized. The nuclear content of DNA for all analyzed Theobroma species was 1C = ~ 0.46 pg. These species presented 2n = 20 with small chromosomes and only one pair of terminal heterochromatic bands positively stained (CMA+/DAPI− bands). The small size of Theobroma ssp. genomes was equivalent to other Byttnerioideae species, suggesting that the basal lineage of Malvaceae have smaller genomes and that there was an expansion of 2C values in the more specialized family clades. A set of 20 EST-SSR primers were characterized for related species of Theobroma, in which 12 loci were polymorphic. The polymorphism information content (PIC) ranged from 0.23 to 0.65, indicating a high level of information per locus. Combined results of flow cytometry, cytogenetic data and EST-SSRs markers will contribute to better describe the species and infer about the evolutionary relationships among Theobroma species. In addition, the importance of a core collection for conservation purposes is highlighted. |
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Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae).Theobroma CacaoTheobromaThe genus Theobroma comprises several trees species native to the Amazon. Theobroma cacao L. plays a key economic role mainly in the chocolate industry. Both cultivated and wild forms are described within the genus. Variations in genome size and chromosome number have been used for prediction purposes including the frequency of interspecific hybridization or inference about evolutionary relationships. In this study, the nuclear DNA content, karyotype and genetic diversity using functional microsatellites (EST-SSR) of seven Theobroma species were characterized. The nuclear content of DNA for all analyzed Theobroma species was 1C = ~ 0.46 pg. These species presented 2n = 20 with small chromosomes and only one pair of terminal heterochromatic bands positively stained (CMA+/DAPI− bands). The small size of Theobroma ssp. genomes was equivalent to other Byttnerioideae species, suggesting that the basal lineage of Malvaceae have smaller genomes and that there was an expansion of 2C values in the more specialized family clades. A set of 20 EST-SSR primers were characterized for related species of Theobroma, in which 12 loci were polymorphic. The polymorphism information content (PIC) ranged from 0.23 to 0.65, indicating a high level of information per locus. Combined results of flow cytometry, cytogenetic data and EST-SSRs markers will contribute to better describe the species and infer about the evolutionary relationships among Theobroma species. In addition, the importance of a core collection for conservation purposes is highlighted.RANGELINE AZEVEDO DA SILVA, UESC; GUSTAVO SOUZA, UFPE; LÍVIA SANTOS LIMA LEMOS, UFSB; UILSON VANDERLEI LOPES, CEPLAC/CEPEC; NARA GEÓRGIA RIBEIRO BRAZ PATROCÌNIO, UESC; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; DIDIER CLEMENT, CEPLAC/CEPEC; FABIENNE MICHELI, UESC; KARINA PERES GRAMACHO, CEPLAC/CEPEC.SILVA, R. A. daSOUZA, G.LEMOS, L. S. L.LOPES, U. V.PATROCÌNIO, N. G. R. B.ALVES, R. M.MARCELLINO, L. H.CLEMENT, D.MICHELI, F.GRAMACHO, K. P.2020-06-18T04:12:36Z2020-06-18T04:12:36Z2020-06-172017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePLoS ONE, v. 12, n. 2, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112332110.1371/journal.pone.0170799enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-06-18T04:12:44Zoai:www.alice.cnptia.embrapa.br:doc/1123321Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-06-18T04:12:44falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-06-18T04:12:44Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). |
title |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). |
spellingShingle |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). SILVA, R. A. da Theobroma Cacao Theobroma |
title_short |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). |
title_full |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). |
title_fullStr |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). |
title_full_unstemmed |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). |
title_sort |
Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae). |
author |
SILVA, R. A. da |
author_facet |
SILVA, R. A. da SOUZA, G. LEMOS, L. S. L. LOPES, U. V. PATROCÌNIO, N. G. R. B. ALVES, R. M. MARCELLINO, L. H. CLEMENT, D. MICHELI, F. GRAMACHO, K. P. |
author_role |
author |
author2 |
SOUZA, G. LEMOS, L. S. L. LOPES, U. V. PATROCÌNIO, N. G. R. B. ALVES, R. M. MARCELLINO, L. H. CLEMENT, D. MICHELI, F. GRAMACHO, K. P. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RANGELINE AZEVEDO DA SILVA, UESC; GUSTAVO SOUZA, UFPE; LÍVIA SANTOS LIMA LEMOS, UFSB; UILSON VANDERLEI LOPES, CEPLAC/CEPEC; NARA GEÓRGIA RIBEIRO BRAZ PATROCÌNIO, UESC; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; DIDIER CLEMENT, CEPLAC/CEPEC; FABIENNE MICHELI, UESC; KARINA PERES GRAMACHO, CEPLAC/CEPEC. |
dc.contributor.author.fl_str_mv |
SILVA, R. A. da SOUZA, G. LEMOS, L. S. L. LOPES, U. V. PATROCÌNIO, N. G. R. B. ALVES, R. M. MARCELLINO, L. H. CLEMENT, D. MICHELI, F. GRAMACHO, K. P. |
dc.subject.por.fl_str_mv |
Theobroma Cacao Theobroma |
topic |
Theobroma Cacao Theobroma |
description |
The genus Theobroma comprises several trees species native to the Amazon. Theobroma cacao L. plays a key economic role mainly in the chocolate industry. Both cultivated and wild forms are described within the genus. Variations in genome size and chromosome number have been used for prediction purposes including the frequency of interspecific hybridization or inference about evolutionary relationships. In this study, the nuclear DNA content, karyotype and genetic diversity using functional microsatellites (EST-SSR) of seven Theobroma species were characterized. The nuclear content of DNA for all analyzed Theobroma species was 1C = ~ 0.46 pg. These species presented 2n = 20 with small chromosomes and only one pair of terminal heterochromatic bands positively stained (CMA+/DAPI− bands). The small size of Theobroma ssp. genomes was equivalent to other Byttnerioideae species, suggesting that the basal lineage of Malvaceae have smaller genomes and that there was an expansion of 2C values in the more specialized family clades. A set of 20 EST-SSR primers were characterized for related species of Theobroma, in which 12 loci were polymorphic. The polymorphism information content (PIC) ranged from 0.23 to 0.65, indicating a high level of information per locus. Combined results of flow cytometry, cytogenetic data and EST-SSRs markers will contribute to better describe the species and infer about the evolutionary relationships among Theobroma species. In addition, the importance of a core collection for conservation purposes is highlighted. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2020-06-18T04:12:36Z 2020-06-18T04:12:36Z 2020-06-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
PLoS ONE, v. 12, n. 2, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123321 10.1371/journal.pone.0170799 |
identifier_str_mv |
PLoS ONE, v. 12, n. 2, 2017. 10.1371/journal.pone.0170799 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1123321 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503493213487104 |