Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061481 http://dx.doi.org/10.1371/journal.pone.0160646 |
Resumo: | Cupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality. |
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Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum.GenotiposCupuaçuCupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality.Lucas Ferraz dos Santos, Universidade Estadual de Santa Cruz; Raner José Santana Silva, Universidade Estadual de Santa Cruz; Daniel Oliveira Jordão do Amaral, Universidade Estadual de Santa Cruz; Márcia Fabiana Barbosa de Paula, Universidade Estadual de Santa Cruz; LOENI LUDKE FALCAO, Cenargen; Thierry Legavre, CIRAD; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; Fabienne Micheli, Universidade Estadual de Santa Cruz / CIRAD.SANTOS, L. F. dosSILVA, R. J. S.AMARAL, D. O. J. doPAULA, M. F. B. deFALCÃO, L. L.LEGAVRE, T.ALVES, R. M.MARCELLINO, L. H.MICHELI, F.2017-01-23T11:11:11Z2017-01-23T11:11:11Z2017-01-2320162017-02-22T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 11, n. 8, e0160646, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061481http://dx.doi.org/10.1371/journal.pone.0160646enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:04:24Zoai:www.alice.cnptia.embrapa.br:doc/1061481Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:04:24falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:04:24Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
title |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
spellingShingle |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. SANTOS, L. F. dos Genotipos Cupuaçu |
title_short |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
title_full |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
title_fullStr |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
title_full_unstemmed |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
title_sort |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
author |
SANTOS, L. F. dos |
author_facet |
SANTOS, L. F. dos SILVA, R. J. S. AMARAL, D. O. J. do PAULA, M. F. B. de FALCÃO, L. L. LEGAVRE, T. ALVES, R. M. MARCELLINO, L. H. MICHELI, F. |
author_role |
author |
author2 |
SILVA, R. J. S. AMARAL, D. O. J. do PAULA, M. F. B. de FALCÃO, L. L. LEGAVRE, T. ALVES, R. M. MARCELLINO, L. H. MICHELI, F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Lucas Ferraz dos Santos, Universidade Estadual de Santa Cruz; Raner José Santana Silva, Universidade Estadual de Santa Cruz; Daniel Oliveira Jordão do Amaral, Universidade Estadual de Santa Cruz; Márcia Fabiana Barbosa de Paula, Universidade Estadual de Santa Cruz; LOENI LUDKE FALCAO, Cenargen; Thierry Legavre, CIRAD; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; Fabienne Micheli, Universidade Estadual de Santa Cruz / CIRAD. |
dc.contributor.author.fl_str_mv |
SANTOS, L. F. dos SILVA, R. J. S. AMARAL, D. O. J. do PAULA, M. F. B. de FALCÃO, L. L. LEGAVRE, T. ALVES, R. M. MARCELLINO, L. H. MICHELI, F. |
dc.subject.por.fl_str_mv |
Genotipos Cupuaçu |
topic |
Genotipos Cupuaçu |
description |
Cupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-01-23T11:11:11Z 2017-01-23T11:11:11Z 2017-01-23 2017-02-22T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 11, n. 8, e0160646, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061481 http://dx.doi.org/10.1371/journal.pone.0160646 |
identifier_str_mv |
Plos One, v. 11, n. 8, e0160646, 2016. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1061481 http://dx.doi.org/10.1371/journal.pone.0160646 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503431254179840 |