The structure of microbial populations in Nelore GIT reveals inter-dependency of methanogens in feces and rumen.

• Autor(a) principal: ANDRADE, B. G. N.
• Data de Publicação: 2020
• Outros Autores: DONATONI, F. A. B., CUADRAT, R. R. C., TIZIOTO, P. C., OLIVEIRA, P. S. N. de, MOURÃO, G. B., COUTINHO, L. L., REECY, J. M., KOLTES, J. E., WALSH, P., BERNDT, A., PALHARES, J. C. P., REGITANO, L. C. de A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121594; https://doi.org/10.1186/s40104-019-0422-x
• topic: Microbiota; Metabarcoding; Bactéria; Bos Indicus; Gado Nelore; Archaea; Methanobrevibacter; Microbiome

The success of different species of ruminants in the colonization of a diverse range of environments is due to their ability to digest and absorb nutrients from cellulose, a complex polysaccharide found in leaves and grass. Ruminants rely on a complex and diverse microbial community, or microbiota, in a unique compartment known as the rumen to break down this polysaccharide. Changes in microbial populations of the rumen can affect the host?s development, health, and productivity. However, accessing the rumen is stressful for the animal. Therefore, the development and use of alternative sampling methods are needed if this technique is to be routinely used in cattle breeding. To this end, we tested if the fecal microbiome could be used as a proxy for the rumen microbiome due to its accessibility. We investigated the taxonomic composition, diversity and inter-relations of two different GIT compartments, rumen and feces, of 26 Nelore (Bos indicus) bulls, using Next Generation Sequencing (NGS) metabarcoding of bacteria, archaea and ciliate protozoa.