Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156992 |
Resumo: | Fipronil is an insecticide widely used in the agricultural and veterinary sectors for its efficacy in pest control. The presence of fipronil in the environment is mainly due to agricultural and domestic practices and is frequently found in different types of environmental matrices in concentrations ranging from µg/L to mg/L and can be hazardous to non-target organisms due to its high toxicity. This study was carried out to obtain and characterize microorganisms from soil which are capable of biodegrading fipronil that could be of great biotechnological interest. For this purpose, bioprospecting was carried out using fipronil (0.6 g/L) as the main source of carbon and nitrogen for growth. Once obtained, the strain was identified by sequencing the 16S ribosomal RNA (rRNA) gene and the capacity to degrade fipronil was monitored by GC-MS. Our study showed a presence in soil samples of the strain identified as Enterobacter chengduensis, which was able to metabolize fipronil and its metabolites during the mineralization process. Enterobacter chengduensis was able to biodegrade fipronil (96%) and its metabolites fipronil-sulfone (92%) and fipronil-sulfide (79%) in 14 days. Overall, the results of this study provided a bacterium with great potential that could contribute to the degradation of fipronil in the environment. |
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Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective.PesticidaBiodegradaçãoFipronil is an insecticide widely used in the agricultural and veterinary sectors for its efficacy in pest control. The presence of fipronil in the environment is mainly due to agricultural and domestic practices and is frequently found in different types of environmental matrices in concentrations ranging from µg/L to mg/L and can be hazardous to non-target organisms due to its high toxicity. This study was carried out to obtain and characterize microorganisms from soil which are capable of biodegrading fipronil that could be of great biotechnological interest. For this purpose, bioprospecting was carried out using fipronil (0.6 g/L) as the main source of carbon and nitrogen for growth. Once obtained, the strain was identified by sequencing the 16S ribosomal RNA (rRNA) gene and the capacity to degrade fipronil was monitored by GC-MS. Our study showed a presence in soil samples of the strain identified as Enterobacter chengduensis, which was able to metabolize fipronil and its metabolites during the mineralization process. Enterobacter chengduensis was able to biodegrade fipronil (96%) and its metabolites fipronil-sulfone (92%) and fipronil-sulfide (79%) in 14 days. Overall, the results of this study provided a bacterium with great potential that could contribute to the degradation of fipronil in the environment.CAIO PRADO, UNIVERSIDADE DE SÃO PAULO, FACULDADE DE ENGENHARIA, LORENA, SP; RODRIGO PEREIRA, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS; LUCIA DURRANT, BioSage, Lawreceville, NJ, USA; ROMULO PENNA SCORZA JUNIOR, CPAO; FRANCINE PIUBELI, University of Seville, Pharmacy Faculty, Seville, Spain; MARICI BONFÁ, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS.PRADO, C.PEREIRA, R.DURRANT, L.SCORZA JUNIOR, R. P.PIUBELI, F.BONFÁ, M.2023-09-30T03:22:22Z2023-09-30T03:22:22Z2023-09-292023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLife, v. 13, n. 9, p. 1935, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/115699210.3390/life13091935enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-30T03:22:22Zoai:www.alice.cnptia.embrapa.br:doc/1156992Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-30T03:22:22falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-30T03:22:22Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
title |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
spellingShingle |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. PRADO, C. Pesticida Biodegradação |
title_short |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
title_full |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
title_fullStr |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
title_full_unstemmed |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
title_sort |
Fipronil Degradation in Soil by Enterobacter chengduensis Strain G2.8: Metabolic Perspective. |
author |
PRADO, C. |
author_facet |
PRADO, C. PEREIRA, R. DURRANT, L. SCORZA JUNIOR, R. P. PIUBELI, F. BONFÁ, M. |
author_role |
author |
author2 |
PEREIRA, R. DURRANT, L. SCORZA JUNIOR, R. P. PIUBELI, F. BONFÁ, M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
CAIO PRADO, UNIVERSIDADE DE SÃO PAULO, FACULDADE DE ENGENHARIA, LORENA, SP; RODRIGO PEREIRA, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS; LUCIA DURRANT, BioSage, Lawreceville, NJ, USA; ROMULO PENNA SCORZA JUNIOR, CPAO; FRANCINE PIUBELI, University of Seville, Pharmacy Faculty, Seville, Spain; MARICI BONFÁ, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS, DOURADOS. |
dc.contributor.author.fl_str_mv |
PRADO, C. PEREIRA, R. DURRANT, L. SCORZA JUNIOR, R. P. PIUBELI, F. BONFÁ, M. |
dc.subject.por.fl_str_mv |
Pesticida Biodegradação |
topic |
Pesticida Biodegradação |
description |
Fipronil is an insecticide widely used in the agricultural and veterinary sectors for its efficacy in pest control. The presence of fipronil in the environment is mainly due to agricultural and domestic practices and is frequently found in different types of environmental matrices in concentrations ranging from µg/L to mg/L and can be hazardous to non-target organisms due to its high toxicity. This study was carried out to obtain and characterize microorganisms from soil which are capable of biodegrading fipronil that could be of great biotechnological interest. For this purpose, bioprospecting was carried out using fipronil (0.6 g/L) as the main source of carbon and nitrogen for growth. Once obtained, the strain was identified by sequencing the 16S ribosomal RNA (rRNA) gene and the capacity to degrade fipronil was monitored by GC-MS. Our study showed a presence in soil samples of the strain identified as Enterobacter chengduensis, which was able to metabolize fipronil and its metabolites during the mineralization process. Enterobacter chengduensis was able to biodegrade fipronil (96%) and its metabolites fipronil-sulfone (92%) and fipronil-sulfide (79%) in 14 days. Overall, the results of this study provided a bacterium with great potential that could contribute to the degradation of fipronil in the environment. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-30T03:22:22Z 2023-09-30T03:22:22Z 2023-09-29 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Life, v. 13, n. 9, p. 1935, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156992 10.3390/life13091935 |
identifier_str_mv |
Life, v. 13, n. 9, p. 1935, 2023. 10.3390/life13091935 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156992 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503549857562624 |