Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.

Detalhes bibliográficos
Autor(a) principal: FAJARDO, T. V. M.
Data de Publicação: 2015
Outros Autores: NASCIMENTO, M. B., EIRAS, M., NICKEL, O., PIO-RIBEIRO, G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032952
Resumo: ABSTRACT There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group. Key words: diagnosis, sequencing, Ilarvirus, PNRSV, ApMV, ArMV RESUMO Inexiste caracterização molecular de isolados brasileiros de Prunus necrotic ringspot virus (PNRSV), exceto aqueles de pessegueiros. Neste trabalho, o agente causal do mosaico da roseira foi determinado e os genes das proteínas de movimento (MP) e capsidial (CP) de um isolado de PNRSV de roseira foram molecularmente caracterizados pela primeira vez no Brasil. As sequências completas de nucleotídeos e de aminoácidos deduzidos da MP e da CP foram alinhadas e comparadas com outros isolados. Análises das sequências de nucleotídeos da MP e da CP do isolado brasileiro de PNRSV de roseira e outros isolados da mesma hospedeira revelaram altas identidades de 96,7% e 98,6%, respectivamente, sendo o isolado Rose-Br classificado no grupo PV32. Palavras-chave: diagnose, sequenciamento, Ilarvirus, PNRSV, ApMV, ArMV.
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spelling Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.SequencingApMVArMVDiagnosisPNRSVIlarvirusABSTRACT There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group. Key words: diagnosis, sequencing, Ilarvirus, PNRSV, ApMV, ArMV RESUMO Inexiste caracterização molecular de isolados brasileiros de Prunus necrotic ringspot virus (PNRSV), exceto aqueles de pessegueiros. Neste trabalho, o agente causal do mosaico da roseira foi determinado e os genes das proteínas de movimento (MP) e capsidial (CP) de um isolado de PNRSV de roseira foram molecularmente caracterizados pela primeira vez no Brasil. As sequências completas de nucleotídeos e de aminoácidos deduzidos da MP e da CP foram alinhadas e comparadas com outros isolados. Análises das sequências de nucleotídeos da MP e da CP do isolado brasileiro de PNRSV de roseira e outros isolados da mesma hospedeira revelaram altas identidades de 96,7% e 98,6%, respectivamente, sendo o isolado Rose-Br classificado no grupo PV32. Palavras-chave: diagnose, sequenciamento, Ilarvirus, PNRSV, ApMV, ArMV.THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.FAJARDO, T. V. M.NASCIMENTO, M. B.EIRAS, M.NICKEL, O.PIO-RIBEIRO, G.2016-01-05T11:11:11Z2016-01-05T11:11:11Z2016-01-0520152016-03-17T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCiência Rural, Santa Maria, v. 45, n. 12, p. 2197-2200, dez. 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032952enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:28:39Zoai:www.alice.cnptia.embrapa.br:doc/1032952Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:28:39falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:28:39Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
title Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
spellingShingle Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
FAJARDO, T. V. M.
Sequencing
ApMV
ArMV
Diagnosis
PNRSV
Ilarvirus
title_short Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
title_full Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
title_fullStr Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
title_full_unstemmed Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
title_sort Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil.
author FAJARDO, T. V. M.
author_facet FAJARDO, T. V. M.
NASCIMENTO, M. B.
EIRAS, M.
NICKEL, O.
PIO-RIBEIRO, G.
author_role author
author2 NASCIMENTO, M. B.
EIRAS, M.
NICKEL, O.
PIO-RIBEIRO, G.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv THOR VINICIUS MARTINS FAJARDO, CNPUV; OSMAR NICKEL, CNPUV.
dc.contributor.author.fl_str_mv FAJARDO, T. V. M.
NASCIMENTO, M. B.
EIRAS, M.
NICKEL, O.
PIO-RIBEIRO, G.
dc.subject.por.fl_str_mv Sequencing
ApMV
ArMV
Diagnosis
PNRSV
Ilarvirus
topic Sequencing
ApMV
ArMV
Diagnosis
PNRSV
Ilarvirus
description ABSTRACT There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group. Key words: diagnosis, sequencing, Ilarvirus, PNRSV, ApMV, ArMV RESUMO Inexiste caracterização molecular de isolados brasileiros de Prunus necrotic ringspot virus (PNRSV), exceto aqueles de pessegueiros. Neste trabalho, o agente causal do mosaico da roseira foi determinado e os genes das proteínas de movimento (MP) e capsidial (CP) de um isolado de PNRSV de roseira foram molecularmente caracterizados pela primeira vez no Brasil. As sequências completas de nucleotídeos e de aminoácidos deduzidos da MP e da CP foram alinhadas e comparadas com outros isolados. Análises das sequências de nucleotídeos da MP e da CP do isolado brasileiro de PNRSV de roseira e outros isolados da mesma hospedeira revelaram altas identidades de 96,7% e 98,6%, respectivamente, sendo o isolado Rose-Br classificado no grupo PV32. Palavras-chave: diagnose, sequenciamento, Ilarvirus, PNRSV, ApMV, ArMV.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016-01-05T11:11:11Z
2016-01-05T11:11:11Z
2016-01-05
2016-03-17T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Ciência Rural, Santa Maria, v. 45, n. 12, p. 2197-2200, dez. 2015.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032952
identifier_str_mv Ciência Rural, Santa Maria, v. 45, n. 12, p. 2197-2200, dez. 2015.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1032952
dc.language.iso.fl_str_mv eng
language eng
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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