Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe

Detalhes bibliográficos
Autor(a) principal: Fajardo,Thor Vinícius Martins
Data de Publicação: 2014
Outros Autores: Nickel,Osmar
Tipo de documento: Relatório
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001711
Resumo: The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was sufficiently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specificity was confirmed by reaction with homologous viral cDNA, individually cloned for each virus.
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spelling Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobediagnosisMalusPyruspolyprobeASGVACLSVASPVApMV.The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was sufficiently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specificity was confirmed by reaction with homologous viral cDNA, individually cloned for each virus.Universidade Federal de Santa Maria2014-10-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001711Ciência Rural v.44 n.10 2014reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20131629info:eu-repo/semantics/openAccessFajardo,Thor Vinícius MartinsNickel,Osmareng2014-12-15T00:00:00ZRevista
dc.title.none.fl_str_mv Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
title Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
spellingShingle Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
Fajardo,Thor Vinícius Martins
diagnosis
Malus
Pyrus
polyprobe
ASGV
ACLSV
ASPV
ApMV.
title_short Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
title_full Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
title_fullStr Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
title_full_unstemmed Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
title_sort Simultaneous detection of four viruses affecting apple and pear by molecular hybridization using a polyprobe
author Fajardo,Thor Vinícius Martins
author_facet Fajardo,Thor Vinícius Martins
Nickel,Osmar
author_role author
author2 Nickel,Osmar
author2_role author
dc.contributor.author.fl_str_mv Fajardo,Thor Vinícius Martins
Nickel,Osmar
dc.subject.por.fl_str_mv diagnosis
Malus
Pyrus
polyprobe
ASGV
ACLSV
ASPV
ApMV.
topic diagnosis
Malus
Pyrus
polyprobe
ASGV
ACLSV
ASPV
ApMV.
description The viruses Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV) and Apple mosaic virus (ApMV) are common in apples and pears and main targets of detection in propagation materials. This study aimed at demonstrating the usefulness of the hybridization method with a non-radioactive probe for simultaneous detection of these four viruses. The sensitivity of this method was sufficiently high enabling the detection of ASGV, ACLSV, ASPV and ApMV in total RNA extracted from infected samples. The probe specificity was confirmed by reaction with homologous viral cDNA, individually cloned for each virus.
publishDate 2014
dc.date.none.fl_str_mv 2014-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/report
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format report
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001711
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014001001711
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20131629
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.44 n.10 2014
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140545924497408

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