Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397 |
Resumo: | The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium. |
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Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.Imuno-histoquímicaDesenvolvimento folicularMaturação de oócitosLeptinaImunoexpressão de proteínas receptorasOvinoOvárioThe aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.Taís Jobard S. Macedo; Jamile Maiara S. Santos; Maria Éllida S. Bezerra; Vanúzia G. Menezes; Bruna B. Gouveia; Lara Mariane R. Barbosa; Thae Lane Barbosa G. Lins; Alane Pains O. Monte; Ricássio S. Barberino; André M. Batista; Vanessa Raquel P. Barros; Aurea Wischral; MARIO ADRIANO A. QUEIROZ; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; Maria Helena T. Matos.MACEDO, T. J. S.SANTOS, J. M. S.BEZERRA, M. E. S.MENEZES, V. G.GOUVEIA, B. B.BARBOSA, L. M. R.LINS, T. L. B. GMONTE, A. P. O.BARBERINO, R. S.BATISTA, A. M.BARROS, V. R. P.WISCHRAL, A.QUEIROZ, M. A. A.ARAUJO, G. G. L. deMATOS, M. H. T.2019-11-14T18:28:56Z2019-11-14T18:28:56Z2019-11-1420192019-11-18T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMolecular and Cellular Endocrinology, v. 495, sept. 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/111439710.1016/j.mce.2019.110506enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-11-14T18:29:03Zoai:www.alice.cnptia.embrapa.br:doc/1114397Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-11-14T18:29:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-11-14T18:29:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
title |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
spellingShingle |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. MACEDO, T. J. S. Imuno-histoquímica Desenvolvimento folicular Maturação de oócitos Leptina Imunoexpressão de proteínas receptoras Ovino Ovário |
title_short |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
title_full |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
title_fullStr |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
title_full_unstemmed |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
title_sort |
Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation. |
author |
MACEDO, T. J. S. |
author_facet |
MACEDO, T. J. S. SANTOS, J. M. S. BEZERRA, M. E. S. MENEZES, V. G. GOUVEIA, B. B. BARBOSA, L. M. R. LINS, T. L. B. G MONTE, A. P. O. BARBERINO, R. S. BATISTA, A. M. BARROS, V. R. P. WISCHRAL, A. QUEIROZ, M. A. A. ARAUJO, G. G. L. de MATOS, M. H. T. |
author_role |
author |
author2 |
SANTOS, J. M. S. BEZERRA, M. E. S. MENEZES, V. G. GOUVEIA, B. B. BARBOSA, L. M. R. LINS, T. L. B. G MONTE, A. P. O. BARBERINO, R. S. BATISTA, A. M. BARROS, V. R. P. WISCHRAL, A. QUEIROZ, M. A. A. ARAUJO, G. G. L. de MATOS, M. H. T. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Taís Jobard S. Macedo; Jamile Maiara S. Santos; Maria Éllida S. Bezerra; Vanúzia G. Menezes; Bruna B. Gouveia; Lara Mariane R. Barbosa; Thae Lane Barbosa G. Lins; Alane Pains O. Monte; Ricássio S. Barberino; André M. Batista; Vanessa Raquel P. Barros; Aurea Wischral; MARIO ADRIANO A. QUEIROZ; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; Maria Helena T. Matos. |
dc.contributor.author.fl_str_mv |
MACEDO, T. J. S. SANTOS, J. M. S. BEZERRA, M. E. S. MENEZES, V. G. GOUVEIA, B. B. BARBOSA, L. M. R. LINS, T. L. B. G MONTE, A. P. O. BARBERINO, R. S. BATISTA, A. M. BARROS, V. R. P. WISCHRAL, A. QUEIROZ, M. A. A. ARAUJO, G. G. L. de MATOS, M. H. T. |
dc.subject.por.fl_str_mv |
Imuno-histoquímica Desenvolvimento folicular Maturação de oócitos Leptina Imunoexpressão de proteínas receptoras Ovino Ovário |
topic |
Imuno-histoquímica Desenvolvimento folicular Maturação de oócitos Leptina Imunoexpressão de proteínas receptoras Ovino Ovário |
description |
The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-11-14T18:28:56Z 2019-11-14T18:28:56Z 2019-11-14 2019 2019-11-18T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Molecular and Cellular Endocrinology, v. 495, sept. 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397 10.1016/j.mce.2019.110506 |
identifier_str_mv |
Molecular and Cellular Endocrinology, v. 495, sept. 2019. 10.1016/j.mce.2019.110506 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503483882209280 |