Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.

Detalhes bibliográficos
Autor(a) principal: MACEDO, T. J. S.
Data de Publicação: 2019
Outros Autores: SANTOS, J. M. S., BEZERRA, M. E. S., MENEZES, V. G., GOUVEIA, B. B., BARBOSA, L. M. R., LINS, T. L. B. G, MONTE, A. P. O., BARBERINO, R. S., BATISTA, A. M., BARROS, V. R. P., WISCHRAL, A., QUEIROZ, M. A. A., ARAUJO, G. G. L. de, MATOS, M. H. T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397
Resumo: The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.
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spelling Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.Imuno-histoquímicaDesenvolvimento folicularMaturação de oócitosLeptinaImunoexpressão de proteínas receptorasOvinoOvárioThe aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.Taís Jobard S. Macedo; Jamile Maiara S. Santos; Maria Éllida S. Bezerra; Vanúzia G. Menezes; Bruna B. Gouveia; Lara Mariane R. Barbosa; Thae Lane Barbosa G. Lins; Alane Pains O. Monte; Ricássio S. Barberino; André M. Batista; Vanessa Raquel P. Barros; Aurea Wischral; MARIO ADRIANO A. QUEIROZ; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; Maria Helena T. Matos.MACEDO, T. J. S.SANTOS, J. M. S.BEZERRA, M. E. S.MENEZES, V. G.GOUVEIA, B. B.BARBOSA, L. M. R.LINS, T. L. B. GMONTE, A. P. O.BARBERINO, R. S.BATISTA, A. M.BARROS, V. R. P.WISCHRAL, A.QUEIROZ, M. A. A.ARAUJO, G. G. L. deMATOS, M. H. T.2019-11-14T18:28:56Z2019-11-14T18:28:56Z2019-11-1420192019-11-18T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMolecular and Cellular Endocrinology, v. 495, sept. 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/111439710.1016/j.mce.2019.110506enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-11-14T18:29:03Zoai:www.alice.cnptia.embrapa.br:doc/1114397Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-11-14T18:29:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-11-14T18:29:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
title Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
spellingShingle Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
MACEDO, T. J. S.
Imuno-histoquímica
Desenvolvimento folicular
Maturação de oócitos
Leptina
Imunoexpressão de proteínas receptoras
Ovino
Ovário
title_short Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
title_full Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
title_fullStr Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
title_full_unstemmed Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
title_sort Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.
author MACEDO, T. J. S.
author_facet MACEDO, T. J. S.
SANTOS, J. M. S.
BEZERRA, M. E. S.
MENEZES, V. G.
GOUVEIA, B. B.
BARBOSA, L. M. R.
LINS, T. L. B. G
MONTE, A. P. O.
BARBERINO, R. S.
BATISTA, A. M.
BARROS, V. R. P.
WISCHRAL, A.
QUEIROZ, M. A. A.
ARAUJO, G. G. L. de
MATOS, M. H. T.
author_role author
author2 SANTOS, J. M. S.
BEZERRA, M. E. S.
MENEZES, V. G.
GOUVEIA, B. B.
BARBOSA, L. M. R.
LINS, T. L. B. G
MONTE, A. P. O.
BARBERINO, R. S.
BATISTA, A. M.
BARROS, V. R. P.
WISCHRAL, A.
QUEIROZ, M. A. A.
ARAUJO, G. G. L. de
MATOS, M. H. T.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Taís Jobard S. Macedo; Jamile Maiara S. Santos; Maria Éllida S. Bezerra; Vanúzia G. Menezes; Bruna B. Gouveia; Lara Mariane R. Barbosa; Thae Lane Barbosa G. Lins; Alane Pains O. Monte; Ricássio S. Barberino; André M. Batista; Vanessa Raquel P. Barros; Aurea Wischral; MARIO ADRIANO A. QUEIROZ; GHERMAN GARCIA LEAL DE ARAUJO, CPATSA; Maria Helena T. Matos.
dc.contributor.author.fl_str_mv MACEDO, T. J. S.
SANTOS, J. M. S.
BEZERRA, M. E. S.
MENEZES, V. G.
GOUVEIA, B. B.
BARBOSA, L. M. R.
LINS, T. L. B. G
MONTE, A. P. O.
BARBERINO, R. S.
BATISTA, A. M.
BARROS, V. R. P.
WISCHRAL, A.
QUEIROZ, M. A. A.
ARAUJO, G. G. L. de
MATOS, M. H. T.
dc.subject.por.fl_str_mv Imuno-histoquímica
Desenvolvimento folicular
Maturação de oócitos
Leptina
Imunoexpressão de proteínas receptoras
Ovino
Ovário
topic Imuno-histoquímica
Desenvolvimento folicular
Maturação de oócitos
Leptina
Imunoexpressão de proteínas receptoras
Ovino
Ovário
description The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM+ alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM+. 25 ng/mL leptin showed a higher percentage of MII than the α-MEM+. In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium.
publishDate 2019
dc.date.none.fl_str_mv 2019-11-14T18:28:56Z
2019-11-14T18:28:56Z
2019-11-14
2019
2019-11-18T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Molecular and Cellular Endocrinology, v. 495, sept. 2019.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397
10.1016/j.mce.2019.110506
identifier_str_mv Molecular and Cellular Endocrinology, v. 495, sept. 2019.
10.1016/j.mce.2019.110506
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1114397
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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