Effect of different gonadotropins on in vitro maturation of sheep oocytes.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836 https://doi.org/10.5433/1679-0359.2022v43n6p2731 |
Resumo: | The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes. |
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Effect of different gonadotropins on in vitro maturation of sheep oocytes.Hormônio folículo estimulanteMetafase IIHoechst 33342GSHOócitos ovinosOvinoMaturação ArtificialHormônio AnimalFollicle-stimulating hormoneMetaphaseSheepThe aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.BRUNA DIAS MANGUEIRA BASTOS, UNIVASF; MARIA NAIARA PEREIRA DA SILVA, UNIVASF; PRICILA RAMOS GONÇALVES, UNIVASF; ANA ELISA CUSTÓDIO MONTES CÂNDIDO, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; EDILSON SOARES LOPES JÚNIOR, UNIVASF.BASTOS, B. D. M.SILVA, N. P. da S.GONÇALVES, P. R.CANDIDO, A. E. C. M.BARBERINO, R. de S.MONTE, A. P. O. doNOGUEIRA, D. M.CORDEIRO, M. F.MATOS, M. H. T. deLOPES JUNIOR, E. S.2023-02-17T15:01:40Z2023-02-17T15:01:40Z2023-02-172022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSemina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022.1676-546Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836https://doi.org/10.5433/1679-0359.2022v43n6p2731enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-02-17T15:01:40Zoai:www.alice.cnptia.embrapa.br:doc/1151836Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-02-17T15:01:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-02-17T15:01:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
title |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
spellingShingle |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. BASTOS, B. D. M. Hormônio folículo estimulante Metafase II Hoechst 33342 GSH Oócitos ovinos Ovino Maturação Artificial Hormônio Animal Follicle-stimulating hormone Metaphase Sheep |
title_short |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
title_full |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
title_fullStr |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
title_full_unstemmed |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
title_sort |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
author |
BASTOS, B. D. M. |
author_facet |
BASTOS, B. D. M. SILVA, N. P. da S. GONÇALVES, P. R. CANDIDO, A. E. C. M. BARBERINO, R. de S. MONTE, A. P. O. do NOGUEIRA, D. M. CORDEIRO, M. F. MATOS, M. H. T. de LOPES JUNIOR, E. S. |
author_role |
author |
author2 |
SILVA, N. P. da S. GONÇALVES, P. R. CANDIDO, A. E. C. M. BARBERINO, R. de S. MONTE, A. P. O. do NOGUEIRA, D. M. CORDEIRO, M. F. MATOS, M. H. T. de LOPES JUNIOR, E. S. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
BRUNA DIAS MANGUEIRA BASTOS, UNIVASF; MARIA NAIARA PEREIRA DA SILVA, UNIVASF; PRICILA RAMOS GONÇALVES, UNIVASF; ANA ELISA CUSTÓDIO MONTES CÂNDIDO, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; EDILSON SOARES LOPES JÚNIOR, UNIVASF. |
dc.contributor.author.fl_str_mv |
BASTOS, B. D. M. SILVA, N. P. da S. GONÇALVES, P. R. CANDIDO, A. E. C. M. BARBERINO, R. de S. MONTE, A. P. O. do NOGUEIRA, D. M. CORDEIRO, M. F. MATOS, M. H. T. de LOPES JUNIOR, E. S. |
dc.subject.por.fl_str_mv |
Hormônio folículo estimulante Metafase II Hoechst 33342 GSH Oócitos ovinos Ovino Maturação Artificial Hormônio Animal Follicle-stimulating hormone Metaphase Sheep |
topic |
Hormônio folículo estimulante Metafase II Hoechst 33342 GSH Oócitos ovinos Ovino Maturação Artificial Hormônio Animal Follicle-stimulating hormone Metaphase Sheep |
description |
The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2023-02-17T15:01:40Z 2023-02-17T15:01:40Z 2023-02-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022. 1676-546X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836 https://doi.org/10.5433/1679-0359.2022v43n6p2731 |
identifier_str_mv |
Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022. 1676-546X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836 https://doi.org/10.5433/1679-0359.2022v43n6p2731 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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