Effect of different gonadotropins on in vitro maturation of sheep oocytes.

Detalhes bibliográficos
Autor(a) principal: BASTOS, B. D. M.
Data de Publicação: 2022
Outros Autores: SILVA, N. P. da S., GONÇALVES, P. R., CANDIDO, A. E. C. M., BARBERINO, R. de S., MONTE, A. P. O. do, NOGUEIRA, D. M., CORDEIRO, M. F., MATOS, M. H. T. de, LOPES JUNIOR, E. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836
https://doi.org/10.5433/1679-0359.2022v43n6p2731
Resumo: The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.
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spelling Effect of different gonadotropins on in vitro maturation of sheep oocytes.Hormônio folículo estimulanteMetafase IIHoechst 33342GSHOócitos ovinosOvinoMaturação ArtificialHormônio AnimalFollicle-stimulating hormoneMetaphaseSheepThe aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.BRUNA DIAS MANGUEIRA BASTOS, UNIVASF; MARIA NAIARA PEREIRA DA SILVA, UNIVASF; PRICILA RAMOS GONÇALVES, UNIVASF; ANA ELISA CUSTÓDIO MONTES CÂNDIDO, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; EDILSON SOARES LOPES JÚNIOR, UNIVASF.BASTOS, B. D. M.SILVA, N. P. da S.GONÇALVES, P. R.CANDIDO, A. E. C. M.BARBERINO, R. de S.MONTE, A. P. O. doNOGUEIRA, D. M.CORDEIRO, M. F.MATOS, M. H. T. deLOPES JUNIOR, E. S.2023-02-17T15:01:40Z2023-02-17T15:01:40Z2023-02-172022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSemina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022.1676-546Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836https://doi.org/10.5433/1679-0359.2022v43n6p2731enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-02-17T15:01:40Zoai:www.alice.cnptia.embrapa.br:doc/1151836Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-02-17T15:01:40falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-02-17T15:01:40Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Effect of different gonadotropins on in vitro maturation of sheep oocytes.
title Effect of different gonadotropins on in vitro maturation of sheep oocytes.
spellingShingle Effect of different gonadotropins on in vitro maturation of sheep oocytes.
BASTOS, B. D. M.
Hormônio folículo estimulante
Metafase II
Hoechst 33342
GSH
Oócitos ovinos
Ovino
Maturação Artificial
Hormônio Animal
Follicle-stimulating hormone
Metaphase
Sheep
title_short Effect of different gonadotropins on in vitro maturation of sheep oocytes.
title_full Effect of different gonadotropins on in vitro maturation of sheep oocytes.
title_fullStr Effect of different gonadotropins on in vitro maturation of sheep oocytes.
title_full_unstemmed Effect of different gonadotropins on in vitro maturation of sheep oocytes.
title_sort Effect of different gonadotropins on in vitro maturation of sheep oocytes.
author BASTOS, B. D. M.
author_facet BASTOS, B. D. M.
SILVA, N. P. da S.
GONÇALVES, P. R.
CANDIDO, A. E. C. M.
BARBERINO, R. de S.
MONTE, A. P. O. do
NOGUEIRA, D. M.
CORDEIRO, M. F.
MATOS, M. H. T. de
LOPES JUNIOR, E. S.
author_role author
author2 SILVA, N. P. da S.
GONÇALVES, P. R.
CANDIDO, A. E. C. M.
BARBERINO, R. de S.
MONTE, A. P. O. do
NOGUEIRA, D. M.
CORDEIRO, M. F.
MATOS, M. H. T. de
LOPES JUNIOR, E. S.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv BRUNA DIAS MANGUEIRA BASTOS, UNIVASF; MARIA NAIARA PEREIRA DA SILVA, UNIVASF; PRICILA RAMOS GONÇALVES, UNIVASF; ANA ELISA CUSTÓDIO MONTES CÂNDIDO, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; EDILSON SOARES LOPES JÚNIOR, UNIVASF.
dc.contributor.author.fl_str_mv BASTOS, B. D. M.
SILVA, N. P. da S.
GONÇALVES, P. R.
CANDIDO, A. E. C. M.
BARBERINO, R. de S.
MONTE, A. P. O. do
NOGUEIRA, D. M.
CORDEIRO, M. F.
MATOS, M. H. T. de
LOPES JUNIOR, E. S.
dc.subject.por.fl_str_mv Hormônio folículo estimulante
Metafase II
Hoechst 33342
GSH
Oócitos ovinos
Ovino
Maturação Artificial
Hormônio Animal
Follicle-stimulating hormone
Metaphase
Sheep
topic Hormônio folículo estimulante
Metafase II
Hoechst 33342
GSH
Oócitos ovinos
Ovino
Maturação Artificial
Hormônio Animal
Follicle-stimulating hormone
Metaphase
Sheep
description The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023-02-17T15:01:40Z
2023-02-17T15:01:40Z
2023-02-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022.
1676-546X
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836
https://doi.org/10.5433/1679-0359.2022v43n6p2731
identifier_str_mv Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022.
1676-546X
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151836
https://doi.org/10.5433/1679-0359.2022v43n6p2731
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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