Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/69730 |
Resumo: | Anthracnose disease of common bean (Phaseolus vulgaris), caused by Colletotrichum lindemuthianum, is responsible for extensive yield losses worldwide. This pathogen is known to vary greatly in its pathogenicity. Control strategies include chemical control and, mainly, the development of resistant cultivars, taking into account the population structure of C. lindemuthianum. The objective of this study was to investigate the pathogenic and genetic diversity and population structure among C. lindemuthianum isolates collected in Minas Gerais state, Brazil. When these isolates were inoculated on 12 differential cultivars, a total of 10 races were identified within a series of 48 isolates collected in Minas Gerais, Brazil. Races 65, 81 and 73 were the most frequent races and occurred in most of the regions. This study also detected race 337, which had not been reported previously in the literature. Random amplified polymorphic DNA (RAPD) analysis performed on the same 48 isolates revealed great genetic diversity, clustering the series into five groups at a maximum similarity value of 89.6%. There was no clear relationship between the loci sampled by RAPD markers and the pathogenic characterization. Analysis of molecular variance showed that 96.06% of the variability was contained within regions and 3.94% among regions, indicating a high exchange of genetic material among the regions of the State. Most of the variability was detected within races (75.24%). The pathogenicity and RAPD assays corroborated the broad genetic diversity of the pathogen and the results have been useful in breeding for resistance to anthracnose. |
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Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil.Physiological raceMolecular markerCommon beanRandom amplified polymorphicColletotrichum LindemuthianumDNAPhaseolus VulgarisanthracnoseAnthracnose disease of common bean (Phaseolus vulgaris), caused by Colletotrichum lindemuthianum, is responsible for extensive yield losses worldwide. This pathogen is known to vary greatly in its pathogenicity. Control strategies include chemical control and, mainly, the development of resistant cultivars, taking into account the population structure of C. lindemuthianum. The objective of this study was to investigate the pathogenic and genetic diversity and population structure among C. lindemuthianum isolates collected in Minas Gerais state, Brazil. When these isolates were inoculated on 12 differential cultivars, a total of 10 races were identified within a series of 48 isolates collected in Minas Gerais, Brazil. Races 65, 81 and 73 were the most frequent races and occurred in most of the regions. This study also detected race 337, which had not been reported previously in the literature. Random amplified polymorphic DNA (RAPD) analysis performed on the same 48 isolates revealed great genetic diversity, clustering the series into five groups at a maximum similarity value of 89.6%. There was no clear relationship between the loci sampled by RAPD markers and the pathogenic characterization. Analysis of molecular variance showed that 96.06% of the variability was contained within regions and 3.94% among regions, indicating a high exchange of genetic material among the regions of the State. Most of the variability was detected within races (75.24%). The pathogenicity and RAPD assays corroborated the broad genetic diversity of the pathogen and the results have been useful in breeding for resistance to anthracnose.KAESEL JACKSON DAMASCENO E SILVA, CPAMN.SILVA, K. J. D. eSOUZA, E. A. deISHIKAWA, F. H.2023-09-14T12:23:47Z2023-09-14T12:23:47Z2008-03-222007info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Phytopathology, Berlin, v. 155, n. 4, p. 241-247, 2007.0031-949Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/69730enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-14T12:23:47Zoai:www.alice.cnptia.embrapa.br:doc/69730Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-14T12:23:47falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-14T12:23:47Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. |
title |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. |
spellingShingle |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. SILVA, K. J. D. e Physiological race Molecular marker Common bean Random amplified polymorphic Colletotrichum Lindemuthianum DNA Phaseolus Vulgaris anthracnose |
title_short |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. |
title_full |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. |
title_fullStr |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. |
title_full_unstemmed |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. |
title_sort |
Characterization of Colletotrichum lindemuthianum Isolates from the State of Minas Gerais, Brazil. |
author |
SILVA, K. J. D. e |
author_facet |
SILVA, K. J. D. e SOUZA, E. A. de ISHIKAWA, F. H. |
author_role |
author |
author2 |
SOUZA, E. A. de ISHIKAWA, F. H. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
KAESEL JACKSON DAMASCENO E SILVA, CPAMN. |
dc.contributor.author.fl_str_mv |
SILVA, K. J. D. e SOUZA, E. A. de ISHIKAWA, F. H. |
dc.subject.por.fl_str_mv |
Physiological race Molecular marker Common bean Random amplified polymorphic Colletotrichum Lindemuthianum DNA Phaseolus Vulgaris anthracnose |
topic |
Physiological race Molecular marker Common bean Random amplified polymorphic Colletotrichum Lindemuthianum DNA Phaseolus Vulgaris anthracnose |
description |
Anthracnose disease of common bean (Phaseolus vulgaris), caused by Colletotrichum lindemuthianum, is responsible for extensive yield losses worldwide. This pathogen is known to vary greatly in its pathogenicity. Control strategies include chemical control and, mainly, the development of resistant cultivars, taking into account the population structure of C. lindemuthianum. The objective of this study was to investigate the pathogenic and genetic diversity and population structure among C. lindemuthianum isolates collected in Minas Gerais state, Brazil. When these isolates were inoculated on 12 differential cultivars, a total of 10 races were identified within a series of 48 isolates collected in Minas Gerais, Brazil. Races 65, 81 and 73 were the most frequent races and occurred in most of the regions. This study also detected race 337, which had not been reported previously in the literature. Random amplified polymorphic DNA (RAPD) analysis performed on the same 48 isolates revealed great genetic diversity, clustering the series into five groups at a maximum similarity value of 89.6%. There was no clear relationship between the loci sampled by RAPD markers and the pathogenic characterization. Analysis of molecular variance showed that 96.06% of the variability was contained within regions and 3.94% among regions, indicating a high exchange of genetic material among the regions of the State. Most of the variability was detected within races (75.24%). The pathogenicity and RAPD assays corroborated the broad genetic diversity of the pathogen and the results have been useful in breeding for resistance to anthracnose. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2008-03-22 2023-09-14T12:23:47Z 2023-09-14T12:23:47Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Phytopathology, Berlin, v. 155, n. 4, p. 241-247, 2007. 0031-949X http://www.alice.cnptia.embrapa.br/alice/handle/doc/69730 |
identifier_str_mv |
Journal of Phytopathology, Berlin, v. 155, n. 4, p. 241-247, 2007. 0031-949X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/69730 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503549192765440 |