Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme

Detalhes bibliográficos
Autor(a) principal: Nogueira, G.B.
Data de Publicação: 2013
Outros Autores: Queiroz, M.V., Ribeiro, R.A., Araújo, E.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.4238/2013.February.8.7
http://www.locus.ufv.br/handle/123456789/13050
Resumo: Colletotrichum lindemuthianum is the causal agent of plant bean anthracnose, one of the most important diseases affecting the common bean. We investigated the structure and expression of the nit1 gene (nitrate reductase) of C. lindemuthianum. The nit1 gene open reading frame contains 2787 bp, interrupted by a single 69-bp intron. The predicted protein has 905 amino acids; it shows high identity with the nitrate reductase of C. higginsianum (79%) and C. graminicola (73%). Expression of nit1 in C. lindemuthianum was evaluated in mycelia grown on different nitrogen sources under conditions of activation and repression. The gene was expressed after 15 min of induction with nitrate, reaching maximum expression at 360 min. The transcription was repressed in mycelia grown in media enriched with ammonia, urea or glutamine. Twenty nit1⁻ mutants were obtained in a medium treated with chlorate. Ten of these mutants were characterized by DNA hybridization, which identified point mutations, a deletion and an insertion. These rearrangements in the nit1 gene in the different mutants may have occurred through activity of transposable elements.
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spelling Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzymeColletotrichum lindemuthianumNitrate reductaseAnthracnosePhaseolus vulgarisColletotrichum lindemuthianum is the causal agent of plant bean anthracnose, one of the most important diseases affecting the common bean. We investigated the structure and expression of the nit1 gene (nitrate reductase) of C. lindemuthianum. The nit1 gene open reading frame contains 2787 bp, interrupted by a single 69-bp intron. The predicted protein has 905 amino acids; it shows high identity with the nitrate reductase of C. higginsianum (79%) and C. graminicola (73%). Expression of nit1 in C. lindemuthianum was evaluated in mycelia grown on different nitrogen sources under conditions of activation and repression. The gene was expressed after 15 min of induction with nitrate, reaching maximum expression at 360 min. The transcription was repressed in mycelia grown in media enriched with ammonia, urea or glutamine. Twenty nit1⁻ mutants were obtained in a medium treated with chlorate. Ten of these mutants were characterized by DNA hybridization, which identified point mutations, a deletion and an insertion. These rearrangements in the nit1 gene in the different mutants may have occurred through activity of transposable elements.Genetics and Molecular Research2017-11-14T11:04:08Z2017-11-14T11:04:08Z2013-02-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf16765680http://dx.doi.org/10.4238/2013.February.8.7http://www.locus.ufv.br/handle/123456789/13050eng12(1), p.420-433, Feb. 2013Nogueira, G.B.Queiroz, M.V.Ribeiro, R.A.Araújo, E.F.info:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T07:39:19Zoai:locus.ufv.br:123456789/13050Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T07:39:19LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
title Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
spellingShingle Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
Nogueira, G.B.
Colletotrichum lindemuthianum
Nitrate reductase
Anthracnose
Phaseolus vulgaris
title_short Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
title_full Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
title_fullStr Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
title_full_unstemmed Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
title_sort Structural and functional characterization of the Colletotrichum lindemuthianum nit1 gene, which encodes a nitrate eductase enzyme
author Nogueira, G.B.
author_facet Nogueira, G.B.
Queiroz, M.V.
Ribeiro, R.A.
Araújo, E.F.
author_role author
author2 Queiroz, M.V.
Ribeiro, R.A.
Araújo, E.F.
author2_role author
author
author
dc.contributor.author.fl_str_mv Nogueira, G.B.
Queiroz, M.V.
Ribeiro, R.A.
Araújo, E.F.
dc.subject.por.fl_str_mv Colletotrichum lindemuthianum
Nitrate reductase
Anthracnose
Phaseolus vulgaris
topic Colletotrichum lindemuthianum
Nitrate reductase
Anthracnose
Phaseolus vulgaris
description Colletotrichum lindemuthianum is the causal agent of plant bean anthracnose, one of the most important diseases affecting the common bean. We investigated the structure and expression of the nit1 gene (nitrate reductase) of C. lindemuthianum. The nit1 gene open reading frame contains 2787 bp, interrupted by a single 69-bp intron. The predicted protein has 905 amino acids; it shows high identity with the nitrate reductase of C. higginsianum (79%) and C. graminicola (73%). Expression of nit1 in C. lindemuthianum was evaluated in mycelia grown on different nitrogen sources under conditions of activation and repression. The gene was expressed after 15 min of induction with nitrate, reaching maximum expression at 360 min. The transcription was repressed in mycelia grown in media enriched with ammonia, urea or glutamine. Twenty nit1⁻ mutants were obtained in a medium treated with chlorate. Ten of these mutants were characterized by DNA hybridization, which identified point mutations, a deletion and an insertion. These rearrangements in the nit1 gene in the different mutants may have occurred through activity of transposable elements.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-08
2017-11-14T11:04:08Z
2017-11-14T11:04:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv 16765680
http://dx.doi.org/10.4238/2013.February.8.7
http://www.locus.ufv.br/handle/123456789/13050
identifier_str_mv 16765680
url http://dx.doi.org/10.4238/2013.February.8.7
http://www.locus.ufv.br/handle/123456789/13050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 12(1), p.420-433, Feb. 2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Genetics and Molecular Research
publisher.none.fl_str_mv Genetics and Molecular Research
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
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repository.mail.fl_str_mv fabiojreis@ufv.br
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