Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.

Detalhes bibliográficos
Autor(a) principal: DIAS, M. C.
Data de Publicação: 2022
Outros Autores: BELGACEM, M. N., RESENDE, J. V. de, MARTINS, M. A., DAMÁSIO, R. A. P., TONOLI, G. H. D., FERREIRA, S. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1142273
https://doi.org/10.1016/j.ijbiomac.2022.04.005
Resumo: Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition. The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly. Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes. This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production. The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF. The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule. This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.
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spelling Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.Cell wallCellulose nanofibersNanofibrillated celluloseLignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition. The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly. Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes. This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production. The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF. The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule. This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.MARIA ALICE MARTINS, CNPDIA.DIAS, M. C.BELGACEM, M. N.RESENDE, J. V. deMARTINS, M. A.DAMÁSIO, R. A. P.TONOLI, G. H. D.FERREIRA, S. R.2024-01-24T14:33:03Z2024-01-24T14:33:03Z2022-04-182022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article413-425International Journal of Biological Macromolecules, v. 209, 2022.0141-8130http://www.alice.cnptia.embrapa.br/alice/handle/doc/1142273https://doi.org/10.1016/j.ijbiomac.2022.04.005enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-01-24T14:33:03Zoai:www.alice.cnptia.embrapa.br:doc/1142273Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-01-24T14:33:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-01-24T14:33:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
title Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
spellingShingle Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
DIAS, M. C.
Cell wall
Cellulose nanofibers
Nanofibrillated cellulose
title_short Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
title_full Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
title_fullStr Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
title_full_unstemmed Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
title_sort Eco-friendly laccase and cellulase enzymes pretreatment for optimized production of high content lignin-cellulose nanofibrils.
author DIAS, M. C.
author_facet DIAS, M. C.
BELGACEM, M. N.
RESENDE, J. V. de
MARTINS, M. A.
DAMÁSIO, R. A. P.
TONOLI, G. H. D.
FERREIRA, S. R.
author_role author
author2 BELGACEM, M. N.
RESENDE, J. V. de
MARTINS, M. A.
DAMÁSIO, R. A. P.
TONOLI, G. H. D.
FERREIRA, S. R.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv MARIA ALICE MARTINS, CNPDIA.
dc.contributor.author.fl_str_mv DIAS, M. C.
BELGACEM, M. N.
RESENDE, J. V. de
MARTINS, M. A.
DAMÁSIO, R. A. P.
TONOLI, G. H. D.
FERREIRA, S. R.
dc.subject.por.fl_str_mv Cell wall
Cellulose nanofibers
Nanofibrillated cellulose
topic Cell wall
Cellulose nanofibers
Nanofibrillated cellulose
description Lignin-cellulose nanofibrils (LCNF) are of attracting an increasing interest due to the benefits of maintaining the lignin in the nanomaterial composition. The production of LCNF requires considerable energy consumption, which has been suppressed employing pretreatment of biomass, in which it highlights those that employ enzymes that have the advantage of being more environmentally friendly. Some negative aspects of the presence of lignin in the fiber to obtain cellulose nanofibrils is that it can hinder the delamination of the cell wall and act as a physical barrier to the action of cellulase enzymes. This study aimed to evaluate the impact of a combined enzymatic pretreatment of laccase and endoglucanase for high content lignin LCNF production. The morphological and chemical properties, visual aspect and stability, crystallinity, mechanical properties, rheology, barrier properties and quality index were used to characterize the LCNF. The laccase loading used was efficient in modifying the lignin to facilitate the action of the endoglucanase on cellulose without causing the removal of this macromolecule. This pretreatment improved the quality of LCNF (61 ± 3 to 71 ± 2 points) with an energy saving of 42% and, therefore, this pretreatment could be suitable for industrial production for a variety of applications.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-18
2022
2024-01-24T14:33:03Z
2024-01-24T14:33:03Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv International Journal of Biological Macromolecules, v. 209, 2022.
0141-8130
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1142273
https://doi.org/10.1016/j.ijbiomac.2022.04.005
identifier_str_mv International Journal of Biological Macromolecules, v. 209, 2022.
0141-8130
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1142273
https://doi.org/10.1016/j.ijbiomac.2022.04.005
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 413-425
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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