Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938 https://doi.org/10.14483/2256201X.19696 |
Resumo: | Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA. |
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Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.MangabaHancornia SpeciosaCultura de TecidoTecido VegetalGenética VegetalTropical and subtropical fruitsPlant geneticsTissue cultureHancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA.ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC.LEDO, A. da S.MACHADO, C. de A.OLIVEIRA, A. C. A. deARRIGONI BLANK, M. deCASTRO, E. M. deSILVA, A. V. C. da2023-12-18T20:33:31Z2023-12-18T20:33:31Z2023-12-182023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleColombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938https://doi.org/10.14483/2256201X.19696enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-12-18T20:33:31Zoai:www.alice.cnptia.embrapa.br:doc/1159938Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-12-18T20:33:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
spellingShingle |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. LEDO, A. da S. Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture |
title_short |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_full |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_fullStr |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_full_unstemmed |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
title_sort |
Callogenesis and morphohistological characterization of Hancornia speciosa Gomes. |
author |
LEDO, A. da S. |
author_facet |
LEDO, A. da S. MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da |
author_role |
author |
author2 |
MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC. |
dc.contributor.author.fl_str_mv |
LEDO, A. da S. MACHADO, C. de A. OLIVEIRA, A. C. A. de ARRIGONI BLANK, M. de CASTRO, E. M. de SILVA, A. V. C. da |
dc.subject.por.fl_str_mv |
Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture |
topic |
Mangaba Hancornia Speciosa Cultura de Tecido Tecido Vegetal Genética Vegetal Tropical and subtropical fruits Plant genetics Tissue culture |
description |
Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-18T20:33:31Z 2023-12-18T20:33:31Z 2023-12-18 2023 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Colombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938 https://doi.org/10.14483/2256201X.19696 |
identifier_str_mv |
Colombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938 https://doi.org/10.14483/2256201X.19696 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1822721641384247296 |