Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.

Detalhes bibliográficos
Autor(a) principal: LEDO, A. da S.
Data de Publicação: 2023
Outros Autores: MACHADO, C. de A., OLIVEIRA, A. C. A. de, ARRIGONI BLANK, M. de, CASTRO, E. M. de, SILVA, A. V. C. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938
https://doi.org/10.14483/2256201X.19696
Resumo: Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA.
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spelling Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.MangabaHancornia SpeciosaCultura de TecidoTecido VegetalGenética VegetalTropical and subtropical fruitsPlant geneticsTissue cultureHancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA.ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC.LEDO, A. da S.MACHADO, C. de A.OLIVEIRA, A. C. A. deARRIGONI BLANK, M. deCASTRO, E. M. deSILVA, A. V. C. da2023-12-18T20:33:31Z2023-12-18T20:33:31Z2023-12-182023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleColombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938https://doi.org/10.14483/2256201X.19696enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-12-18T20:33:31Zoai:www.alice.cnptia.embrapa.br:doc/1159938Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-12-18T20:33:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
spellingShingle Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
LEDO, A. da S.
Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
title_short Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_full Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_fullStr Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_full_unstemmed Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
title_sort Callogenesis and morphohistological characterization of Hancornia speciosa Gomes.
author LEDO, A. da S.
author_facet LEDO, A. da S.
MACHADO, C. de A.
OLIVEIRA, A. C. A. de
ARRIGONI BLANK, M. de
CASTRO, E. M. de
SILVA, A. V. C. da
author_role author
author2 MACHADO, C. de A.
OLIVEIRA, A. C. A. de
ARRIGONI BLANK, M. de
CASTRO, E. M. de
SILVA, A. V. C. da
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv ANA DA SILVA LEDO, CPATC; CAROLINE DE ARAUJO MACHADO, UFS; ANNIE CAROLINA ARAÚJO DE OLIVEIRA, UFS; MARIA DE FÁTIMA ARRIGONI BLANK, UFS; EVARISTO MAURO DE CASTRO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC.
dc.contributor.author.fl_str_mv LEDO, A. da S.
MACHADO, C. de A.
OLIVEIRA, A. C. A. de
ARRIGONI BLANK, M. de
CASTRO, E. M. de
SILVA, A. V. C. da
dc.subject.por.fl_str_mv Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
topic Mangaba
Hancornia Speciosa
Cultura de Tecido
Tecido Vegetal
Genética Vegetal
Tropical and subtropical fruits
Plant genetics
Tissue culture
description Hancornia speciosa Gomes belongs to the family Apocynaceae and is distributed across different regions of Brazil. The objective of this study was to evaluate callus induction and histodifferentiation in five mangaba accessions under different in vitro culture conditions. Five acessions from the Active Germplasm Bank of Mangaba (Embrapa, Brazil) were evaluated. In vitro-germinated plant seedlings were used for the excision of different explants (internode and node segments, and foliar section). These segments were inoculated in a culture medium containing different concentrations of 6-Benzylaminopurine (BA) and 2,4- Dichlorophenoxyacetic acid (2,4-D). There was a significant effect of accessions, treatments, and time on the callus mass (g). There was no callus induction in the absence of regulators. After 60 days of in vitro culture, all treatments exhibited a linear positive cellular growth. The highest callus mass was observed in the BI accession, in the presence of 22.62 µM 2,4-D and 11.10 µM BA. Hancornia speciosa Gomes pertenece a la familia Apocynaceae, distribuida en diferentes regiones de Brasil. El objetivo de este trabajo fue evaluar la callogénesis y la histodiferenciación en cinco accesiones de mangaba bajo diferentes condiciones de cultivo in vitro. Se evaluaron cinco accesiones del Banco de Germoplasma Activo de Mangaba (Embrapa, Brasil). Las plántulas germinadas in vitro se utilizaron para la escisión de explantes (segmentos nodales e internodales y secciones foliares). Estos segmentos fueron inoculados en un medio de cultivo que contenía diferentes concentraciones de 6-benzilaminopurina (BA) y ácido 2,4-diclorofenoxiacético (2,4-D). Hubo un efecto significativo de las accesiones, los tratamientos y el tiempo del cultivo en la masa de los callos. No hubo inducción de callos en ausencia de reguladores. A los 60 días de cultivo in vitro, los otros tratamientos presentaron un crecimiento celular linear positivo. La mayor masa de callo se observó en la accesión BI, en presencia de 22,62 μM 2,4-D y 11,10 μM BA.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-18T20:33:31Z
2023-12-18T20:33:31Z
2023-12-18
2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Colombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938
https://doi.org/10.14483/2256201X.19696
identifier_str_mv Colombia Forestal, v. 26, n. 2, p. 5-14, ju./dez. 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1159938
https://doi.org/10.14483/2256201X.19696
dc.language.iso.fl_str_mv eng
language eng
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
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