Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684 |
Resumo: | Hancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli. |
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Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.Fruta TropicalMangabaHancornia SpeciosaTropical and subtropical fruitsHancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli.ADRIELE M. BARBOSA; KLEBSON S. SANTOS; GUSTAVO R. BORGES; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FLAVIA M. R. MENDONCA; MALONE S. PINHEIRO; ELTON FRANCESCHI; CLAUDIO DARIVA; FRANCINE F. PADILHA.BARBOSA, A. M.SANTOS, K. S.BORGES, G. R.MUNIZ, A. V. C. da S.MENDONCA, F. M. R.PINHEIRO, M. S.FRANCESCHI, E.DARIVA, C.PADILHA, F. F.2020-01-14T18:12:36Z2020-01-14T18:12:36Z2020-01-1420192020-01-14T18:12:36Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSeparation and Purification Technology, n. 222, p. 390-395, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-01-14T18:12:42Zoai:www.alice.cnptia.embrapa.br:doc/1118684Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-01-14T18:12:42Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. |
title |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. |
spellingShingle |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. BARBOSA, A. M. Fruta Tropical Mangaba Hancornia Speciosa Tropical and subtropical fruits |
title_short |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. |
title_full |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. |
title_fullStr |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. |
title_full_unstemmed |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. |
title_sort |
Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction. |
author |
BARBOSA, A. M. |
author_facet |
BARBOSA, A. M. SANTOS, K. S. BORGES, G. R. MUNIZ, A. V. C. da S. MENDONCA, F. M. R. PINHEIRO, M. S. FRANCESCHI, E. DARIVA, C. PADILHA, F. F. |
author_role |
author |
author2 |
SANTOS, K. S. BORGES, G. R. MUNIZ, A. V. C. da S. MENDONCA, F. M. R. PINHEIRO, M. S. FRANCESCHI, E. DARIVA, C. PADILHA, F. F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
ADRIELE M. BARBOSA; KLEBSON S. SANTOS; GUSTAVO R. BORGES; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FLAVIA M. R. MENDONCA; MALONE S. PINHEIRO; ELTON FRANCESCHI; CLAUDIO DARIVA; FRANCINE F. PADILHA. |
dc.contributor.author.fl_str_mv |
BARBOSA, A. M. SANTOS, K. S. BORGES, G. R. MUNIZ, A. V. C. da S. MENDONCA, F. M. R. PINHEIRO, M. S. FRANCESCHI, E. DARIVA, C. PADILHA, F. F. |
dc.subject.por.fl_str_mv |
Fruta Tropical Mangaba Hancornia Speciosa Tropical and subtropical fruits |
topic |
Fruta Tropical Mangaba Hancornia Speciosa Tropical and subtropical fruits |
description |
Hancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2020-01-14T18:12:36Z 2020-01-14T18:12:36Z 2020-01-14 2020-01-14T18:12:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Separation and Purification Technology, n. 222, p. 390-395, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684 |
identifier_str_mv |
Separation and Purification Technology, n. 222, p. 390-395, 2019. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1817695582413651968 |