Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.

Detalhes bibliográficos
Autor(a) principal: BARBOSA, A. M.
Data de Publicação: 2019
Outros Autores: SANTOS, K. S., BORGES, G. R., MUNIZ, A. V. C. da S., MENDONCA, F. M. R., PINHEIRO, M. S., FRANCESCHI, E., DARIVA, C., PADILHA, F. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684
Resumo: Hancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli.
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spelling Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.Fruta TropicalMangabaHancornia SpeciosaTropical and subtropical fruitsHancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli.ADRIELE M. BARBOSA; KLEBSON S. SANTOS; GUSTAVO R. BORGES; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FLAVIA M. R. MENDONCA; MALONE S. PINHEIRO; ELTON FRANCESCHI; CLAUDIO DARIVA; FRANCINE F. PADILHA.BARBOSA, A. M.SANTOS, K. S.BORGES, G. R.MUNIZ, A. V. C. da S.MENDONCA, F. M. R.PINHEIRO, M. S.FRANCESCHI, E.DARIVA, C.PADILHA, F. F.2020-01-14T18:12:36Z2020-01-14T18:12:36Z2020-01-1420192020-01-14T18:12:36Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSeparation and Purification Technology, n. 222, p. 390-395, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-01-14T18:12:42Zoai:www.alice.cnptia.embrapa.br:doc/1118684Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-01-14T18:12:42Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
title Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
spellingShingle Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
BARBOSA, A. M.
Fruta Tropical
Mangaba
Hancornia Speciosa
Tropical and subtropical fruits
title_short Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
title_full Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
title_fullStr Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
title_full_unstemmed Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
title_sort Separation of antbacterial biocompounds from Hancorni speciosa leaves by a sequential process of pressurized liquid extraction.
author BARBOSA, A. M.
author_facet BARBOSA, A. M.
SANTOS, K. S.
BORGES, G. R.
MUNIZ, A. V. C. da S.
MENDONCA, F. M. R.
PINHEIRO, M. S.
FRANCESCHI, E.
DARIVA, C.
PADILHA, F. F.
author_role author
author2 SANTOS, K. S.
BORGES, G. R.
MUNIZ, A. V. C. da S.
MENDONCA, F. M. R.
PINHEIRO, M. S.
FRANCESCHI, E.
DARIVA, C.
PADILHA, F. F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv ADRIELE M. BARBOSA; KLEBSON S. SANTOS; GUSTAVO R. BORGES; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; FLAVIA M. R. MENDONCA; MALONE S. PINHEIRO; ELTON FRANCESCHI; CLAUDIO DARIVA; FRANCINE F. PADILHA.
dc.contributor.author.fl_str_mv BARBOSA, A. M.
SANTOS, K. S.
BORGES, G. R.
MUNIZ, A. V. C. da S.
MENDONCA, F. M. R.
PINHEIRO, M. S.
FRANCESCHI, E.
DARIVA, C.
PADILHA, F. F.
dc.subject.por.fl_str_mv Fruta Tropical
Mangaba
Hancornia Speciosa
Tropical and subtropical fruits
topic Fruta Tropical
Mangaba
Hancornia Speciosa
Tropical and subtropical fruits
description Hancornia speciosahas bioactive molecules such as phenolic compounds which exhibit antioxidant and anti-microbial properties. Nevertheless, the separation of these substances from a medicinal plant can be differentaccording to the extraction process and solvent used. Thus, the present study aimed was to separate phenoliccompounds fromHancornia speciosaleaves in three steps by using a sequential pressurized liquid extraction andevaluated their antioxidant and antibacterial activity. Results showed that the temperature increase from 25 to60° C provides a significative enhancement in the extraction yield. The raw ethanol extract (39.0 ± 0.2%)showed the highest overall extraction yield. However, fractionated ethanol extract (FEE) at 60 °C exhibited thehighest amount of total phenolic compounds (347.0 ± 4.12 mg/g extract). Fractionated ethyl acetate extract(FAE) at 60 °C presented improved quantity of totalflavonoids (230.4 ± 3.5 mg/g extract) and rutin content(16.6 ± 0.3 mg/mL extract). The antioxidant activity results for IC50of DPPH indicated that FEE(3.8 ± 0.1 ?g/mL) was the best free radical scavenger. FAE and FEE were able to inhibit the growth of mul-tidrug-resistantE. coli.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-01-14T18:12:36Z
2020-01-14T18:12:36Z
2020-01-14
2020-01-14T18:12:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Separation and Purification Technology, n. 222, p. 390-395, 2019.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684
identifier_str_mv Separation and Purification Technology, n. 222, p. 390-395, 2019.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1118684
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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