Identification of drought-responsive genes in roots of upland rice (Oryza sativa L).
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/217500 https://doi.org/10.1186/1471-2164-9-485 |
Resumo: | Rice (Oryza sativa L.) germplasm represents an extraordinary source of genes that control traits of agronomic importance such as drought tolerance. This diversity is the basis for the development of new cultivars better adapted to water restriction conditions, in particular for upland rice, which is grown under rainfall. The analyses of subtractive cDNA libraries and differential protein expression of drought tolerant and susceptible genotypes can contribute to the understanding of the genetic control of water use efficiency in rice. Two subtractive libraries were constructed using cDNA of drought susceptible and tolerant genotypes submitted to stress against cDNA of well-watered plants. In silico analysis revealed 463 reads, which were grouped into 282 clusters. Several genes expressed exclusively in the tolerant or susceptible genotypes were identified. Additionally, proteome analysis of roots from stressed plants was performed and 22 proteins putatively associated to drought tolerance were identified by mass spectrometry. |
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Identification of drought-responsive genes in roots of upland rice (Oryza sativa L).ArrozGermoplasmaOryza SativaResistência a SecaSistema RadicularriceRice (Oryza sativa L.) germplasm represents an extraordinary source of genes that control traits of agronomic importance such as drought tolerance. This diversity is the basis for the development of new cultivars better adapted to water restriction conditions, in particular for upland rice, which is grown under rainfall. The analyses of subtractive cDNA libraries and differential protein expression of drought tolerant and susceptible genotypes can contribute to the understanding of the genetic control of water use efficiency in rice. Two subtractive libraries were constructed using cDNA of drought susceptible and tolerant genotypes submitted to stress against cDNA of well-watered plants. In silico analysis revealed 463 reads, which were grouped into 282 clusters. Several genes expressed exclusively in the tolerant or susceptible genotypes were identified. Additionally, proteome analysis of roots from stressed plants was performed and 22 proteins putatively associated to drought tolerance were identified by mass spectrometry.ALINE R. RABELLO, UNB; CLEBER MORAIS GUIMARAES, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; F. R. DA SILVA; DANIELA SEIXAS, UFPR; EMANUEL DE SOUZA, UFPR; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CARLOS R. SPEHAR, UNB; MARCIO ELIAS FERREIRA, CENARGEN; ANGELA MEHTA, Cenargen.RABELLO, A. R.GUIMARÃES, C. M.RANGEL, P. H. N.SILVA, F. R. daSEIXAS, D.SOUZA, E. deBRASILEIRO, A. C. M.SPEHAR, C. R.FERREIRA, M. E.MEHTA, A.2022-06-03T05:00:25Z2022-06-03T05:00:25Z2008-11-242008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBMC Genomics, v. 9, n. 485, out. 2008.http://www.alice.cnptia.embrapa.br/alice/handle/doc/217500https://doi.org/10.1186/1471-2164-9-485enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-06-03T05:00:36Zoai:www.alice.cnptia.embrapa.br:doc/217500Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-06-03T05:00:36falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-06-03T05:00:36Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). |
title |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). |
spellingShingle |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). RABELLO, A. R. Arroz Germoplasma Oryza Sativa Resistência a Seca Sistema Radicular rice |
title_short |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). |
title_full |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). |
title_fullStr |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). |
title_full_unstemmed |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). |
title_sort |
Identification of drought-responsive genes in roots of upland rice (Oryza sativa L). |
author |
RABELLO, A. R. |
author_facet |
RABELLO, A. R. GUIMARÃES, C. M. RANGEL, P. H. N. SILVA, F. R. da SEIXAS, D. SOUZA, E. de BRASILEIRO, A. C. M. SPEHAR, C. R. FERREIRA, M. E. MEHTA, A. |
author_role |
author |
author2 |
GUIMARÃES, C. M. RANGEL, P. H. N. SILVA, F. R. da SEIXAS, D. SOUZA, E. de BRASILEIRO, A. C. M. SPEHAR, C. R. FERREIRA, M. E. MEHTA, A. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ALINE R. RABELLO, UNB; CLEBER MORAIS GUIMARAES, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; F. R. DA SILVA; DANIELA SEIXAS, UFPR; EMANUEL DE SOUZA, UFPR; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CARLOS R. SPEHAR, UNB; MARCIO ELIAS FERREIRA, CENARGEN; ANGELA MEHTA, Cenargen. |
dc.contributor.author.fl_str_mv |
RABELLO, A. R. GUIMARÃES, C. M. RANGEL, P. H. N. SILVA, F. R. da SEIXAS, D. SOUZA, E. de BRASILEIRO, A. C. M. SPEHAR, C. R. FERREIRA, M. E. MEHTA, A. |
dc.subject.por.fl_str_mv |
Arroz Germoplasma Oryza Sativa Resistência a Seca Sistema Radicular rice |
topic |
Arroz Germoplasma Oryza Sativa Resistência a Seca Sistema Radicular rice |
description |
Rice (Oryza sativa L.) germplasm represents an extraordinary source of genes that control traits of agronomic importance such as drought tolerance. This diversity is the basis for the development of new cultivars better adapted to water restriction conditions, in particular for upland rice, which is grown under rainfall. The analyses of subtractive cDNA libraries and differential protein expression of drought tolerant and susceptible genotypes can contribute to the understanding of the genetic control of water use efficiency in rice. Two subtractive libraries were constructed using cDNA of drought susceptible and tolerant genotypes submitted to stress against cDNA of well-watered plants. In silico analysis revealed 463 reads, which were grouped into 282 clusters. Several genes expressed exclusively in the tolerant or susceptible genotypes were identified. Additionally, proteome analysis of roots from stressed plants was performed and 22 proteins putatively associated to drought tolerance were identified by mass spectrometry. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-11-24 2008 2022-06-03T05:00:25Z 2022-06-03T05:00:25Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BMC Genomics, v. 9, n. 485, out. 2008. http://www.alice.cnptia.embrapa.br/alice/handle/doc/217500 https://doi.org/10.1186/1471-2164-9-485 |
identifier_str_mv |
BMC Genomics, v. 9, n. 485, out. 2008. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/217500 https://doi.org/10.1186/1471-2164-9-485 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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