Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079819 |
Resumo: | Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatellite (or simple sequence repeat - SSR) molecular markers. Morphological characterization was carried out using 57 descriptors, and molecular characterization was based on 239 alleles from 24 microsatellite loci. From the estimates of genetic distances among accessions, based on molecular characterization, a cluster analysis was carried out, and a dendrogram was established. Correlations between morphological and molecular variables were also estimated. Twelve morphological descriptors were monomorphic for the set of C. frutescens accessions, and those with the highest degree of polymorphism were stem length (14.0 to 62.0 cm), stem diameter (1.0 to 4.2 cm), days to flowering (90 to 129), days to fruiting (100 to 140), fruit weight (0.1 to 1.4 g), fruit length (0.6 to 4.6 cm), and fruit wall thickness (0.25 to 1.5 mm). The polymorphism information content for the SSR loci varied from 0.36 (EPMS 417) to 0.75 (CA49), with an overall mean of 0.57. The correlation value between morphological and molecular characterization data was 0.6604, which was statistically significant. Fourteen accessions were described as belonging to the morphological type tabasco, 85 were described as malagueta, and four were malaguetinha, a morphological type confirmed in this study. The typical morphological pattern of malagueta was described. Six similarity groups were established for C. frutescens based on the dendrogram and are discussed individually. The genetic variability analyzed in the study highlights the importance of characterizing genetic resources available for the development of new C. frutescens cultivars with the potential for various niche markets. |
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Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers.MicrosatellitesPimentaMarcador molecularPeppersPolymorphismCapsicum frutescensGenetic markersCharacterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatellite (or simple sequence repeat - SSR) molecular markers. Morphological characterization was carried out using 57 descriptors, and molecular characterization was based on 239 alleles from 24 microsatellite loci. From the estimates of genetic distances among accessions, based on molecular characterization, a cluster analysis was carried out, and a dendrogram was established. Correlations between morphological and molecular variables were also estimated. Twelve morphological descriptors were monomorphic for the set of C. frutescens accessions, and those with the highest degree of polymorphism were stem length (14.0 to 62.0 cm), stem diameter (1.0 to 4.2 cm), days to flowering (90 to 129), days to fruiting (100 to 140), fruit weight (0.1 to 1.4 g), fruit length (0.6 to 4.6 cm), and fruit wall thickness (0.25 to 1.5 mm). The polymorphism information content for the SSR loci varied from 0.36 (EPMS 417) to 0.75 (CA49), with an overall mean of 0.57. The correlation value between morphological and molecular characterization data was 0.6604, which was statistically significant. Fourteen accessions were described as belonging to the morphological type tabasco, 85 were described as malagueta, and four were malaguetinha, a morphological type confirmed in this study. The typical morphological pattern of malagueta was described. Six similarity groups were established for C. frutescens based on the dendrogram and are discussed individually. The genetic variability analyzed in the study highlights the importance of characterizing genetic resources available for the development of new C. frutescens cultivars with the potential for various niche markets.SABRINA ISABEL COSTA DE CARVALHO, CNPH; LUCIANO DE BEM BIANCHETTI, Cenargen; CARLOS FRANCISCO RAGASSI, CNPH; CLAUDIA SILVA DA COSTA RIBEIRO, CNPH; FRANCISCO JOSE BECKER REIFSCHNEIDER, SRI; GLAUCIA SALLES CORTOPASSI BUSO, Cenargen; FABIO GELAPE FALEIRO, CPAC.CARVALHO, S. I. C.BIANCHETTI, L. B.RAGASSI, C. F.RIBEIRO, C. S. C.REIFSCHNEIDER, F. J. B.BUSO, G. S. C.FALEIRO, F. G.2017-11-16T23:25:39Z2017-11-16T23:25:39Z2017-11-1620172017-11-16T23:25:39Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 16, n. 3, 2017. gmr16039689.1676-5680http://www.alice.cnptia.embrapa.br/alice/handle/doc/107981910.4238/gmr16039689enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-11-16T23:25:46Zoai:www.alice.cnptia.embrapa.br:doc/1079819Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-11-16T23:25:46falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-11-16T23:25:46Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. |
title |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. |
spellingShingle |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. CARVALHO, S. I. C. Microsatellites Pimenta Marcador molecular Peppers Polymorphism Capsicum frutescens Genetic markers |
title_short |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. |
title_full |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. |
title_fullStr |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. |
title_full_unstemmed |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. |
title_sort |
Genetic variability of a Brazilian Capsicum frutescens germplasm collection using morphological characteristics and SSR markers. |
author |
CARVALHO, S. I. C. |
author_facet |
CARVALHO, S. I. C. BIANCHETTI, L. B. RAGASSI, C. F. RIBEIRO, C. S. C. REIFSCHNEIDER, F. J. B. BUSO, G. S. C. FALEIRO, F. G. |
author_role |
author |
author2 |
BIANCHETTI, L. B. RAGASSI, C. F. RIBEIRO, C. S. C. REIFSCHNEIDER, F. J. B. BUSO, G. S. C. FALEIRO, F. G. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
SABRINA ISABEL COSTA DE CARVALHO, CNPH; LUCIANO DE BEM BIANCHETTI, Cenargen; CARLOS FRANCISCO RAGASSI, CNPH; CLAUDIA SILVA DA COSTA RIBEIRO, CNPH; FRANCISCO JOSE BECKER REIFSCHNEIDER, SRI; GLAUCIA SALLES CORTOPASSI BUSO, Cenargen; FABIO GELAPE FALEIRO, CPAC. |
dc.contributor.author.fl_str_mv |
CARVALHO, S. I. C. BIANCHETTI, L. B. RAGASSI, C. F. RIBEIRO, C. S. C. REIFSCHNEIDER, F. J. B. BUSO, G. S. C. FALEIRO, F. G. |
dc.subject.por.fl_str_mv |
Microsatellites Pimenta Marcador molecular Peppers Polymorphism Capsicum frutescens Genetic markers |
topic |
Microsatellites Pimenta Marcador molecular Peppers Polymorphism Capsicum frutescens Genetic markers |
description |
Characterization studies provide essential information for the conservation and use of germplasm in plant breeding programs. In this study, 103 Capsicum frutescens L. accessions from the Active Germplasm Bank of Embrapa Hortaliças, representative of all five Brazilian geographic regions, were characterized based on morphological characteristics and microsatellite (or simple sequence repeat - SSR) molecular markers. Morphological characterization was carried out using 57 descriptors, and molecular characterization was based on 239 alleles from 24 microsatellite loci. From the estimates of genetic distances among accessions, based on molecular characterization, a cluster analysis was carried out, and a dendrogram was established. Correlations between morphological and molecular variables were also estimated. Twelve morphological descriptors were monomorphic for the set of C. frutescens accessions, and those with the highest degree of polymorphism were stem length (14.0 to 62.0 cm), stem diameter (1.0 to 4.2 cm), days to flowering (90 to 129), days to fruiting (100 to 140), fruit weight (0.1 to 1.4 g), fruit length (0.6 to 4.6 cm), and fruit wall thickness (0.25 to 1.5 mm). The polymorphism information content for the SSR loci varied from 0.36 (EPMS 417) to 0.75 (CA49), with an overall mean of 0.57. The correlation value between morphological and molecular characterization data was 0.6604, which was statistically significant. Fourteen accessions were described as belonging to the morphological type tabasco, 85 were described as malagueta, and four were malaguetinha, a morphological type confirmed in this study. The typical morphological pattern of malagueta was described. Six similarity groups were established for C. frutescens based on the dendrogram and are discussed individually. The genetic variability analyzed in the study highlights the importance of characterizing genetic resources available for the development of new C. frutescens cultivars with the potential for various niche markets. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-16T23:25:39Z 2017-11-16T23:25:39Z 2017-11-16 2017 2017-11-16T23:25:39Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 16, n. 3, 2017. gmr16039689. 1676-5680 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079819 10.4238/gmr16039689 |
identifier_str_mv |
Genetics and Molecular Research, v. 16, n. 3, 2017. gmr16039689. 1676-5680 10.4238/gmr16039689 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1079819 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503444650786816 |