Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.

Detalhes bibliográficos
Autor(a) principal: PEREIRA, R.
Data de Publicação: 2019
Outros Autores: ABREU, A. de F. B., NALIN, R. S., SOUZA, E. A. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121768
Resumo: Phenotyping for severity of angular leaf spot (ALS) in common bean is important to identify new sources of resistance and select progenies in conventional and molecular marker-assisted breeding. In this study, three phenotyping methods for ALS severity were assessed and its implications in breeding for resistance to Pseudocercospora griseola are discussed. Reaction of 144 common bean lines to P. griseola was evaluated. Three different experiments were conducted in the greenhouse (V2 and V3 stages) and in the field. Common bean lines were inoculated with a mixture of spores of pathogen races 63-63 and 63-23. We observed that 31 %, 7 % and 10 % of lines were resistant in stages V2, V3 and in the field, respectively. Estimates of coincidence index V2-V3, V2-field and V3-field were 68 %, 69 % and 88 %, respectively. Evaluations in V3 stage and in the field were the phenotyping methods more efficient for ALS severity. However, evaluation in V3 stage is impracticable to assess routinely many genotypes in breeding programs. Evaluation in V2 stage may be used to carry out a first screening, especially in the early stages of breeding programs. Moreover, this methodology may be used in association with evaluation of plants in the field to increase genetic gain.
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spelling Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.Pseudocercospora griseolaFeijãoPhaseolus VulgarisResistência GenéticaInoculação ArtificialMelhoramento Genético VegetalBeansGenetic resistanceInoculation methodsPseudocercosporaPlant breedingPhenotyping for severity of angular leaf spot (ALS) in common bean is important to identify new sources of resistance and select progenies in conventional and molecular marker-assisted breeding. In this study, three phenotyping methods for ALS severity were assessed and its implications in breeding for resistance to Pseudocercospora griseola are discussed. Reaction of 144 common bean lines to P. griseola was evaluated. Three different experiments were conducted in the greenhouse (V2 and V3 stages) and in the field. Common bean lines were inoculated with a mixture of spores of pathogen races 63-63 and 63-23. We observed that 31 %, 7 % and 10 % of lines were resistant in stages V2, V3 and in the field, respectively. Estimates of coincidence index V2-V3, V2-field and V3-field were 68 %, 69 % and 88 %, respectively. Evaluations in V3 stage and in the field were the phenotyping methods more efficient for ALS severity. However, evaluation in V3 stage is impracticable to assess routinely many genotypes in breeding programs. Evaluation in V2 stage may be used to carry out a first screening, especially in the early stages of breeding programs. Moreover, this methodology may be used in association with evaluation of plants in the field to increase genetic gain.RAFAEL PEREIRA, UFLA; ANGELA DE FATIMA BARBOSA ABREU, CNPAF; RAFAEL STORTO NALIN, ESALQ; ELAINE APARECIDA DE SOUZA, UFLA.PEREIRA, R.ABREU, A. de F. B.NALIN, R. S.SOUZA, E. A. de2020-04-23T00:53:17Z2020-04-23T00:53:17Z2020-04-2220192020-04-23T00:53:17Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleScientia Agricola, v. 76, n. 5, p. 415-423, Sept./Oct. 2019.1678-992Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/112176810.1590/1678-992X-2017-0394enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-04-23T00:53:24Zoai:www.alice.cnptia.embrapa.br:doc/1121768Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-04-23T00:53:24falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-04-23T00:53:24Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
title Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
spellingShingle Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
PEREIRA, R.
Pseudocercospora griseola
Feijão
Phaseolus Vulgaris
Resistência Genética
Inoculação Artificial
Melhoramento Genético Vegetal
Beans
Genetic resistance
Inoculation methods
Pseudocercospora
Plant breeding
title_short Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
title_full Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
title_fullStr Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
title_full_unstemmed Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
title_sort Phenotyping for angular leaf spot severity and its implication in breeding common bean for resistance.
author PEREIRA, R.
author_facet PEREIRA, R.
ABREU, A. de F. B.
NALIN, R. S.
SOUZA, E. A. de
author_role author
author2 ABREU, A. de F. B.
NALIN, R. S.
SOUZA, E. A. de
author2_role author
author
author
dc.contributor.none.fl_str_mv RAFAEL PEREIRA, UFLA; ANGELA DE FATIMA BARBOSA ABREU, CNPAF; RAFAEL STORTO NALIN, ESALQ; ELAINE APARECIDA DE SOUZA, UFLA.
dc.contributor.author.fl_str_mv PEREIRA, R.
ABREU, A. de F. B.
NALIN, R. S.
SOUZA, E. A. de
dc.subject.por.fl_str_mv Pseudocercospora griseola
Feijão
Phaseolus Vulgaris
Resistência Genética
Inoculação Artificial
Melhoramento Genético Vegetal
Beans
Genetic resistance
Inoculation methods
Pseudocercospora
Plant breeding
topic Pseudocercospora griseola
Feijão
Phaseolus Vulgaris
Resistência Genética
Inoculação Artificial
Melhoramento Genético Vegetal
Beans
Genetic resistance
Inoculation methods
Pseudocercospora
Plant breeding
description Phenotyping for severity of angular leaf spot (ALS) in common bean is important to identify new sources of resistance and select progenies in conventional and molecular marker-assisted breeding. In this study, three phenotyping methods for ALS severity were assessed and its implications in breeding for resistance to Pseudocercospora griseola are discussed. Reaction of 144 common bean lines to P. griseola was evaluated. Three different experiments were conducted in the greenhouse (V2 and V3 stages) and in the field. Common bean lines were inoculated with a mixture of spores of pathogen races 63-63 and 63-23. We observed that 31 %, 7 % and 10 % of lines were resistant in stages V2, V3 and in the field, respectively. Estimates of coincidence index V2-V3, V2-field and V3-field were 68 %, 69 % and 88 %, respectively. Evaluations in V3 stage and in the field were the phenotyping methods more efficient for ALS severity. However, evaluation in V3 stage is impracticable to assess routinely many genotypes in breeding programs. Evaluation in V2 stage may be used to carry out a first screening, especially in the early stages of breeding programs. Moreover, this methodology may be used in association with evaluation of plants in the field to increase genetic gain.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-04-23T00:53:17Z
2020-04-23T00:53:17Z
2020-04-22
2020-04-23T00:53:17Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Scientia Agricola, v. 76, n. 5, p. 415-423, Sept./Oct. 2019.
1678-992X
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121768
10.1590/1678-992X-2017-0394
identifier_str_mv Scientia Agricola, v. 76, n. 5, p. 415-423, Sept./Oct. 2019.
1678-992X
10.1590/1678-992X-2017-0394
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1121768
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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