Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance

Detalhes bibliográficos
Autor(a) principal: Pereira, Rafael
Data de Publicação: 2019
Outros Autores: Abreu, Ângela de Fátima Barbosa, Nalin, Rafael Storto, Souza, Elaine Aparecida de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFLA
Texto Completo: http://repositorio.ufla.br/jspui/handle/1/39880
Resumo: Phenotyping for severity of angular leaf spot (ALS) in common bean is important to identify new sources of resistance and select progenies in conventional and molecular marker-assisted breeding. In this study, three phenotyping methods for ALS severity were assessed and its implications in breeding for resistance to Pseudocercospora griseola are discussed. Reaction of 144 common bean lines to P. griseola was evaluated. Three different experiments were conducted in the greenhouse (V2 and V3 stages) and in the field. Common bean lines were inoculated with a mixture of spores of pathogen races 63-63 and 63-23. We observed that 31 %, 7 % and 10 % of lines were resistant in stages V2, V3 and in the field, respectively. Estimates of coincidence index V2-V3, V2-field and V3-field were 68 %, 69 % and 88 %, respectively. Evaluations in V3 stage and in the field were the phenotyping methods more efficient for ALS severity. However, evaluation in V3 stage is impracticable to assess routinely many genotypes in breeding programs. Evaluation in V2 stage may be used to carry out a first screening, especially in the early stages of breeding programs. Moreover, this methodology may be used in association with evaluation of plants in the field to increase genetic gain.
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spelling Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistancePhaseolus vulgaris L.Pseudocercospora griseolaGenetic resistanceArtificial inoculationPhenotyping for severity of angular leaf spot (ALS) in common bean is important to identify new sources of resistance and select progenies in conventional and molecular marker-assisted breeding. In this study, three phenotyping methods for ALS severity were assessed and its implications in breeding for resistance to Pseudocercospora griseola are discussed. Reaction of 144 common bean lines to P. griseola was evaluated. Three different experiments were conducted in the greenhouse (V2 and V3 stages) and in the field. Common bean lines were inoculated with a mixture of spores of pathogen races 63-63 and 63-23. We observed that 31 %, 7 % and 10 % of lines were resistant in stages V2, V3 and in the field, respectively. Estimates of coincidence index V2-V3, V2-field and V3-field were 68 %, 69 % and 88 %, respectively. Evaluations in V3 stage and in the field were the phenotyping methods more efficient for ALS severity. However, evaluation in V3 stage is impracticable to assess routinely many genotypes in breeding programs. Evaluation in V2 stage may be used to carry out a first screening, especially in the early stages of breeding programs. Moreover, this methodology may be used in association with evaluation of plants in the field to increase genetic gain.Escola Superior de Agricultura "Luiz de Queiroz"2020-04-08T13:41:39Z2020-04-08T13:41:39Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfPEREIRA, R. et al. Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance. Scientia Agricola, Piracicaba, v. 76, n. 5, p. 415-423, Sept./Oct. 2019.http://repositorio.ufla.br/jspui/handle/1/39880Scientia Agricolareponame:Repositório Institucional da UFLAinstname:Universidade Federal de Lavras (UFLA)instacron:UFLAAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessPereira, RafaelAbreu, Ângela de Fátima BarbosaNalin, Rafael StortoSouza, Elaine Aparecida deeng2020-04-08T13:42:45Zoai:localhost:1/39880Repositório InstitucionalPUBhttp://repositorio.ufla.br/oai/requestnivaldo@ufla.br || repositorio.biblioteca@ufla.bropendoar:2020-04-08T13:42:45Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)false
dc.title.none.fl_str_mv Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
title Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
spellingShingle Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
Pereira, Rafael
Phaseolus vulgaris L.
Pseudocercospora griseola
Genetic resistance
Artificial inoculation
title_short Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
title_full Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
title_fullStr Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
title_full_unstemmed Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
title_sort Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance
author Pereira, Rafael
author_facet Pereira, Rafael
Abreu, Ângela de Fátima Barbosa
Nalin, Rafael Storto
Souza, Elaine Aparecida de
author_role author
author2 Abreu, Ângela de Fátima Barbosa
Nalin, Rafael Storto
Souza, Elaine Aparecida de
author2_role author
author
author
dc.contributor.author.fl_str_mv Pereira, Rafael
Abreu, Ângela de Fátima Barbosa
Nalin, Rafael Storto
Souza, Elaine Aparecida de
dc.subject.por.fl_str_mv Phaseolus vulgaris L.
Pseudocercospora griseola
Genetic resistance
Artificial inoculation
topic Phaseolus vulgaris L.
Pseudocercospora griseola
Genetic resistance
Artificial inoculation
description Phenotyping for severity of angular leaf spot (ALS) in common bean is important to identify new sources of resistance and select progenies in conventional and molecular marker-assisted breeding. In this study, three phenotyping methods for ALS severity were assessed and its implications in breeding for resistance to Pseudocercospora griseola are discussed. Reaction of 144 common bean lines to P. griseola was evaluated. Three different experiments were conducted in the greenhouse (V2 and V3 stages) and in the field. Common bean lines were inoculated with a mixture of spores of pathogen races 63-63 and 63-23. We observed that 31 %, 7 % and 10 % of lines were resistant in stages V2, V3 and in the field, respectively. Estimates of coincidence index V2-V3, V2-field and V3-field were 68 %, 69 % and 88 %, respectively. Evaluations in V3 stage and in the field were the phenotyping methods more efficient for ALS severity. However, evaluation in V3 stage is impracticable to assess routinely many genotypes in breeding programs. Evaluation in V2 stage may be used to carry out a first screening, especially in the early stages of breeding programs. Moreover, this methodology may be used in association with evaluation of plants in the field to increase genetic gain.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020-04-08T13:41:39Z
2020-04-08T13:41:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv PEREIRA, R. et al. Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance. Scientia Agricola, Piracicaba, v. 76, n. 5, p. 415-423, Sept./Oct. 2019.
http://repositorio.ufla.br/jspui/handle/1/39880
identifier_str_mv PEREIRA, R. et al. Phenotyping for angular leaf spot severity and its implication in common bean breeding for resistance. Scientia Agricola, Piracicaba, v. 76, n. 5, p. 415-423, Sept./Oct. 2019.
url http://repositorio.ufla.br/jspui/handle/1/39880
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
publisher.none.fl_str_mv Escola Superior de Agricultura "Luiz de Queiroz"
dc.source.none.fl_str_mv Scientia Agricola
reponame:Repositório Institucional da UFLA
instname:Universidade Federal de Lavras (UFLA)
instacron:UFLA
instname_str Universidade Federal de Lavras (UFLA)
instacron_str UFLA
institution UFLA
reponame_str Repositório Institucional da UFLA
collection Repositório Institucional da UFLA
repository.name.fl_str_mv Repositório Institucional da UFLA - Universidade Federal de Lavras (UFLA)
repository.mail.fl_str_mv nivaldo@ufla.br || repositorio.biblioteca@ufla.br
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