A Mad7 system for genetic engineering of filamentous fungi.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151460 https://doi.org/10.3390/jof9010016 |
Resumo: | The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories. |
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A Mad7 system for genetic engineering of filamentous fungi.Filamentous fungiCRISPRMad7Fungal strain engineeringFungoAspergillusGenetic engineeringFungiThe introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories.KATHERINA GARCIA VANEGAS, UNIVERSITY OF DENAMARK; JAKOB KRÆMMER HAAR RENDSVIG, UNIVERSITY OF DENMARK; ZOFIA DOROTA JARCZYNSKA, UNIVERSITY OF DENMARK; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ABEL PETER VAN ESCH, UNIVERSITY OF DENAMARK; MARTÍ MORERA-GÓMEZ, UNIVERSITY OF DENAMARK; FABIANO JARES CONTESINI, UNIVERSITY OF DENAMARK; UFFE HASBRO MORTENSEN, UNIVERSITY OF DENAMARK.VANEGAS K. G.RENDSVIG, J. K. H.JARCZYSKA, Z. D.CÔRTES, M. V. de C. B.VAN ESCH, A. P.MORERA-GÓMEZ, M.CONTESINI, F. J.MORTENSEN, U. H.2023-02-02T16:01:20Z2023-02-02T16:01:20Z2023-02-022023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Fungi, v. 9, n. 1, 16, 2023.2309-608Xhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1151460https://doi.org/10.3390/jof9010016enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-02-02T16:01:20Zoai:www.alice.cnptia.embrapa.br:doc/1151460Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-02-02T16:01:20falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-02-02T16:01:20Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
A Mad7 system for genetic engineering of filamentous fungi. |
title |
A Mad7 system for genetic engineering of filamentous fungi. |
spellingShingle |
A Mad7 system for genetic engineering of filamentous fungi. VANEGAS K. G. Filamentous fungi CRISPR Mad7 Fungal strain engineering Fungo Aspergillus Genetic engineering Fungi |
title_short |
A Mad7 system for genetic engineering of filamentous fungi. |
title_full |
A Mad7 system for genetic engineering of filamentous fungi. |
title_fullStr |
A Mad7 system for genetic engineering of filamentous fungi. |
title_full_unstemmed |
A Mad7 system for genetic engineering of filamentous fungi. |
title_sort |
A Mad7 system for genetic engineering of filamentous fungi. |
author |
VANEGAS K. G. |
author_facet |
VANEGAS K. G. RENDSVIG, J. K. H. JARCZYSKA, Z. D. CÔRTES, M. V. de C. B. VAN ESCH, A. P. MORERA-GÓMEZ, M. CONTESINI, F. J. MORTENSEN, U. H. |
author_role |
author |
author2 |
RENDSVIG, J. K. H. JARCZYSKA, Z. D. CÔRTES, M. V. de C. B. VAN ESCH, A. P. MORERA-GÓMEZ, M. CONTESINI, F. J. MORTENSEN, U. H. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
KATHERINA GARCIA VANEGAS, UNIVERSITY OF DENAMARK; JAKOB KRÆMMER HAAR RENDSVIG, UNIVERSITY OF DENMARK; ZOFIA DOROTA JARCZYNSKA, UNIVERSITY OF DENMARK; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ABEL PETER VAN ESCH, UNIVERSITY OF DENAMARK; MARTÍ MORERA-GÓMEZ, UNIVERSITY OF DENAMARK; FABIANO JARES CONTESINI, UNIVERSITY OF DENAMARK; UFFE HASBRO MORTENSEN, UNIVERSITY OF DENAMARK. |
dc.contributor.author.fl_str_mv |
VANEGAS K. G. RENDSVIG, J. K. H. JARCZYSKA, Z. D. CÔRTES, M. V. de C. B. VAN ESCH, A. P. MORERA-GÓMEZ, M. CONTESINI, F. J. MORTENSEN, U. H. |
dc.subject.por.fl_str_mv |
Filamentous fungi CRISPR Mad7 Fungal strain engineering Fungo Aspergillus Genetic engineering Fungi |
topic |
Filamentous fungi CRISPR Mad7 Fungal strain engineering Fungo Aspergillus Genetic engineering Fungi |
description |
The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-02-02T16:01:20Z 2023-02-02T16:01:20Z 2023-02-02 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Fungi, v. 9, n. 1, 16, 2023. 2309-608X http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151460 https://doi.org/10.3390/jof9010016 |
identifier_str_mv |
Journal of Fungi, v. 9, n. 1, 16, 2023. 2309-608X |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1151460 https://doi.org/10.3390/jof9010016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503539424231424 |