Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.

Detalhes bibliográficos
Autor(a) principal: DINIZ, W. J. DA S.
Data de Publicação: 2015
Outros Autores: TIZIOTO, P. C., COUTINHO, L. L., CESAR, A. S. M., GROMBONI, C. F., NOGUEIRA, A. R. de A., REGITANO, L. C. de A.
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154455
Resumo: Iron is essential to several biological processes, such as oxygen transport and lipid metabolism. In this study, the RNA sequencing technology (RNAseq) was adopted to identify differentially expressed genes (DE) and metabolic pathways, as well as its relationship with meat quality parameters influenced by the variation in iron content (IC) in muscle. Samples of Longissimus dorsi (LD) muscle from Nelore cattle (n = 8), extreme genomic estimated breeding value (GEBV) for IC (four samples with high (HIL) and four low (LIL) GEBV) were used. Data analysis by Tuxedo Suite identified 49 annotated genes DE between the groups. The functional enrichment analysis identified important metabolic pathways associated with lipid metabolism, like stearate biosynthesis, fatty acid biosynthesis and palmitate biosynthesis, which participate in the gene FASN, FABP4, SLC27A6 and THRSP. These results suggest biologic pathways influenced by iron and contribute information for the understanding of their participation in the process that affects muscle physiology in cattle.
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spelling Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.Iron metabolismLongissimus dorsiFatty acidsGene expressionIron is essential to several biological processes, such as oxygen transport and lipid metabolism. In this study, the RNA sequencing technology (RNAseq) was adopted to identify differentially expressed genes (DE) and metabolic pathways, as well as its relationship with meat quality parameters influenced by the variation in iron content (IC) in muscle. Samples of Longissimus dorsi (LD) muscle from Nelore cattle (n = 8), extreme genomic estimated breeding value (GEBV) for IC (four samples with high (HIL) and four low (LIL) GEBV) were used. Data analysis by Tuxedo Suite identified 49 annotated genes DE between the groups. The functional enrichment analysis identified important metabolic pathways associated with lipid metabolism, like stearate biosynthesis, fatty acid biosynthesis and palmitate biosynthesis, which participate in the gene FASN, FABP4, SLC27A6 and THRSP. These results suggest biologic pathways influenced by iron and contribute information for the understanding of their participation in the process that affects muscle physiology in cattle.WELLISON JARLES DA SILVA DINIZ, Federal University of São Carlos; POLYANA CRISTINE TIZIOTO; LUIZ LEHMANN COUTINHO, University of São Paulo; ALINE SILVA MELLO CESAR, University of São Paulo; CAIO FERNANDO GROMBONI, Bahia Federal Institute of Education; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.DINIZ, W. J. DA S.TIZIOTO, P. C.COUTINHO, L. L.CESAR, A. S. M.GROMBONI, C. F.NOGUEIRA, A. R. de A.REGITANO, L. C. de A.2023-06-15T18:24:38Z2023-06-15T18:24:38Z2023-06-152015Artigo em anais e proceedingsinfo:eu-repo/semantics/publishedVersionIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 52., Belo Horizonte, 2015. Anais... Belo Horizonte: SBZ, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154455enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-06-15T18:24:38Zoai:www.alice.cnptia.embrapa.br:doc/1154455Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-06-15T18:24:38Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
title Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
spellingShingle Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
DINIZ, W. J. DA S.
Iron metabolism
Longissimus dorsi
Fatty acids
Gene expression
title_short Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
title_full Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
title_fullStr Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
title_full_unstemmed Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
title_sort Transcriptomic analysis identifies differentially expressed genes related to lipid metabolism in extreme animals for GEBV for iron content in the muscle Nelore steers.
author DINIZ, W. J. DA S.
author_facet DINIZ, W. J. DA S.
TIZIOTO, P. C.
COUTINHO, L. L.
CESAR, A. S. M.
GROMBONI, C. F.
NOGUEIRA, A. R. de A.
REGITANO, L. C. de A.
author_role author
author2 TIZIOTO, P. C.
COUTINHO, L. L.
CESAR, A. S. M.
GROMBONI, C. F.
NOGUEIRA, A. R. de A.
REGITANO, L. C. de A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv WELLISON JARLES DA SILVA DINIZ, Federal University of São Carlos; POLYANA CRISTINE TIZIOTO; LUIZ LEHMANN COUTINHO, University of São Paulo; ALINE SILVA MELLO CESAR, University of São Paulo; CAIO FERNANDO GROMBONI, Bahia Federal Institute of Education; ANA RITA DE ARAUJO NOGUEIRA, CPPSE; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
dc.contributor.author.fl_str_mv DINIZ, W. J. DA S.
TIZIOTO, P. C.
COUTINHO, L. L.
CESAR, A. S. M.
GROMBONI, C. F.
NOGUEIRA, A. R. de A.
REGITANO, L. C. de A.
dc.subject.por.fl_str_mv Iron metabolism
Longissimus dorsi
Fatty acids
Gene expression
topic Iron metabolism
Longissimus dorsi
Fatty acids
Gene expression
description Iron is essential to several biological processes, such as oxygen transport and lipid metabolism. In this study, the RNA sequencing technology (RNAseq) was adopted to identify differentially expressed genes (DE) and metabolic pathways, as well as its relationship with meat quality parameters influenced by the variation in iron content (IC) in muscle. Samples of Longissimus dorsi (LD) muscle from Nelore cattle (n = 8), extreme genomic estimated breeding value (GEBV) for IC (four samples with high (HIL) and four low (LIL) GEBV) were used. Data analysis by Tuxedo Suite identified 49 annotated genes DE between the groups. The functional enrichment analysis identified important metabolic pathways associated with lipid metabolism, like stearate biosynthesis, fatty acid biosynthesis and palmitate biosynthesis, which participate in the gene FASN, FABP4, SLC27A6 and THRSP. These results suggest biologic pathways influenced by iron and contribute information for the understanding of their participation in the process that affects muscle physiology in cattle.
publishDate 2015
dc.date.none.fl_str_mv 2015
2023-06-15T18:24:38Z
2023-06-15T18:24:38Z
2023-06-15
dc.type.driver.fl_str_mv Artigo em anais e proceedings
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 52., Belo Horizonte, 2015. Anais... Belo Horizonte: SBZ, 2015.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154455
identifier_str_mv In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 52., Belo Horizonte, 2015. Anais... Belo Horizonte: SBZ, 2015.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154455
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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