Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.

Detalhes bibliográficos
Autor(a) principal: COSTA, M. L. M.
Data de Publicação: 2014
Outros Autores: LANA, U. G. P., BARROS, E. C., PAIVA, L. V., VALICENTE, F. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/985917
Resumo: Bacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed.
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spelling Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.BacteriaControle biológicoPraga de plantaMilhoBiological controlPlant pestsBacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed.UBIRACI GOMES DE PAULA LANA, CNPMS; FERNANDO HERCOS VALICENTE, CNPMS.COSTA, M. L. M.LANA, U. G. P.BARROS, E. C.PAIVA, L. V.VALICENTE, F. H.2018-04-13T01:10:25Z2018-04-13T01:10:25Z2014-05-1220142018-04-13T01:10:25Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Agricultural Science, Cambridge, v. 6, n. 7, p. 1-10, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/98591710.5539/jas.v6n7pxxenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-04-13T01:10:32Zoai:www.alice.cnptia.embrapa.br:doc/985917Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-04-13T01:10:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-04-13T01:10:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
title Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
spellingShingle Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
COSTA, M. L. M.
Bacteria
Controle biológico
Praga de planta
Milho
Biological control
Plant pests
title_short Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
title_full Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
title_fullStr Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
title_full_unstemmed Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
title_sort Molecular characterization of bacillus thuringiensis cyt genes efficient against fall armyworm, Spodoptera frugiperda.
author COSTA, M. L. M.
author_facet COSTA, M. L. M.
LANA, U. G. P.
BARROS, E. C.
PAIVA, L. V.
VALICENTE, F. H.
author_role author
author2 LANA, U. G. P.
BARROS, E. C.
PAIVA, L. V.
VALICENTE, F. H.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UBIRACI GOMES DE PAULA LANA, CNPMS; FERNANDO HERCOS VALICENTE, CNPMS.
dc.contributor.author.fl_str_mv COSTA, M. L. M.
LANA, U. G. P.
BARROS, E. C.
PAIVA, L. V.
VALICENTE, F. H.
dc.subject.por.fl_str_mv Bacteria
Controle biológico
Praga de planta
Milho
Biological control
Plant pests
topic Bacteria
Controle biológico
Praga de planta
Milho
Biological control
Plant pests
description Bacillus thuringiensis (Bt) endotoxins (Cyt and Cry) have been extensively explored for biological control of fall armyworm, Spodoptera frugiperda, an important pest in major corn-producing countries of America. Five hundred Bt isolates with different toxicities against S. frugiperda were characterized by PCR for the presence of cyt genes (cyt1, cyt2, cyt1Aa, cyt1Ab, cyt2B and cyt2Ba), and the effect of insecticidal proteins Cry1Ba, Cry1Ca, Cry1Da and Cyt on S. frugiperda larvae were evaluated. Six isolates showed the presence of cyt genes, three isolates harbored two gene families (cyt1 and cyt2), and three isolates harbored only one of the cyt gene families (cyt1 and cyt2). It was not possible to correlate the presence/absence of cyt genes with toxicity against S. frugiperda. In this study, cyt genes were present in toxic and nontoxic isolates to this insect pest. Bioassays against S. frugiperda larvae showed that only Cry1Ca protein had toxicity, with 77.08% of mortality. Synergism among Cry and Cry proteins used in this study against S. frugiperda was not observed.
publishDate 2014
dc.date.none.fl_str_mv 2014-05-12
2014
2018-04-13T01:10:25Z
2018-04-13T01:10:25Z
2018-04-13T01:10:25Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal of Agricultural Science, Cambridge, v. 6, n. 7, p. 1-10, 2014.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/985917
10.5539/jas.v6n7pxx
identifier_str_mv Journal of Agricultural Science, Cambridge, v. 6, n. 7, p. 1-10, 2014.
10.5539/jas.v6n7pxx
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/985917
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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