Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057399 |
Resumo: | Native of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. Due to its economic potential, there is an interest to advance the genetic breeding program of this species. The aim of this study was to characterize the BAG nanche collection using inter-simple sequence repeat (ISSR) markers. Accessions were genotyped using 23 pre-selected ISSR primers resulting in 109 amplified polymorphic and 51 monomorphic bands. With eight polymorphic bands each, the most polymorphic primers were UBC 809 and UBC 848. An unweighted pair-group method with arithmetic average cluster analysis based on Jaccard?s coefficient indicated that the individuals clustered into two distinct groups. Accessions Igarapé Açu-2 and Augusto Corrêa-Pl 1 were most similar. The genetic dissimilarity values ranged from 0.10 to 0.59. We conclude that the ISSR markers were efficient in detecting polymorphisms in the nanche accessions, and that it is possible to infer the genetic variability among accessions of the collection. This demonstrate the importance of using molecular markers in poorly studied species and the advantages that this information can bring to the genetic improvement of such species. |
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Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers.Marcador MolecularMuriciMalpighiaceaeNative of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. Due to its economic potential, there is an interest to advance the genetic breeding program of this species. The aim of this study was to characterize the BAG nanche collection using inter-simple sequence repeat (ISSR) markers. Accessions were genotyped using 23 pre-selected ISSR primers resulting in 109 amplified polymorphic and 51 monomorphic bands. With eight polymorphic bands each, the most polymorphic primers were UBC 809 and UBC 848. An unweighted pair-group method with arithmetic average cluster analysis based on Jaccard?s coefficient indicated that the individuals clustered into two distinct groups. Accessions Igarapé Açu-2 and Augusto Corrêa-Pl 1 were most similar. The genetic dissimilarity values ranged from 0.10 to 0.59. We conclude that the ISSR markers were efficient in detecting polymorphisms in the nanche accessions, and that it is possible to infer the genetic variability among accessions of the collection. This demonstrate the importance of using molecular markers in poorly studied species and the advantages that this information can bring to the genetic improvement of such species.SIMONE DE MIRANDA RODRIGUES, CPATU; ELISA FERREIRA MOURA CUNHA, CPATU; G. K. S. Ramos, UFRA; MARIA DO SOCORRO P DE OLIVEIRA, CPATU.RODRIGUES, S. M.MOURA, E. F.RAMOS, G. K. S.OLIVEIRA, M. S. P.2016-11-29T11:11:11Z2016-11-29T11:11:11Z2016-11-2920162016-11-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 15, n. 4, article gmr15048887, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/105739910.4238/gmr15048887enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:52:54Zoai:www.alice.cnptia.embrapa.br:doc/1057399Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:52:54falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:52:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. |
title |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. |
spellingShingle |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. RODRIGUES, S. M. Marcador Molecular Murici Malpighiaceae |
title_short |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. |
title_full |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. |
title_fullStr |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. |
title_full_unstemmed |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. |
title_sort |
Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers. |
author |
RODRIGUES, S. M. |
author_facet |
RODRIGUES, S. M. MOURA, E. F. RAMOS, G. K. S. OLIVEIRA, M. S. P. |
author_role |
author |
author2 |
MOURA, E. F. RAMOS, G. K. S. OLIVEIRA, M. S. P. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
SIMONE DE MIRANDA RODRIGUES, CPATU; ELISA FERREIRA MOURA CUNHA, CPATU; G. K. S. Ramos, UFRA; MARIA DO SOCORRO P DE OLIVEIRA, CPATU. |
dc.contributor.author.fl_str_mv |
RODRIGUES, S. M. MOURA, E. F. RAMOS, G. K. S. OLIVEIRA, M. S. P. |
dc.subject.por.fl_str_mv |
Marcador Molecular Murici Malpighiaceae |
topic |
Marcador Molecular Murici Malpighiaceae |
description |
Native of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. Due to its economic potential, there is an interest to advance the genetic breeding program of this species. The aim of this study was to characterize the BAG nanche collection using inter-simple sequence repeat (ISSR) markers. Accessions were genotyped using 23 pre-selected ISSR primers resulting in 109 amplified polymorphic and 51 monomorphic bands. With eight polymorphic bands each, the most polymorphic primers were UBC 809 and UBC 848. An unweighted pair-group method with arithmetic average cluster analysis based on Jaccard?s coefficient indicated that the individuals clustered into two distinct groups. Accessions Igarapé Açu-2 and Augusto Corrêa-Pl 1 were most similar. The genetic dissimilarity values ranged from 0.10 to 0.59. We conclude that the ISSR markers were efficient in detecting polymorphisms in the nanche accessions, and that it is possible to infer the genetic variability among accessions of the collection. This demonstrate the importance of using molecular markers in poorly studied species and the advantages that this information can bring to the genetic improvement of such species. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-29T11:11:11Z 2016-11-29T11:11:11Z 2016-11-29 2016 2016-11-29T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 15, n. 4, article gmr15048887, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057399 10.4238/gmr15048887 |
identifier_str_mv |
Genetics and Molecular Research, v. 15, n. 4, article gmr15048887, 2016. 10.4238/gmr15048887 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057399 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503429110890496 |