Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir).
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126452 |
Resumo: | Endophytic microorganisms have a wide genetic diversity, being very useful for agriculture, for producing growth regulators and making nutrients available to plants. In this context, this research aimed to isolate and characterize endophytic bacteria from bamboo Guadua sp., in order to evaluate the use of these microorganisms in promoting plant growth in yam plants (Dioscorea rotundata Poir). For this, the macromorphological characteristics of bacterial colonies were studied: shape, borders, consistency, color, brightness. Micromorphological analysis was performed using the Gram method. Biological tests of phosphate solubilization, biological nitrogen fixation and indole acetic acid production were carried out, where the bacteria with the best results were selected to assess the potential to promote the growth of white yam plants. The growth experiment consisted of four treatments: a control (without inoculation) and three treatments with endophytic bacteria, inoculated in pre-acclimated yam plants. Each treatment consisted of 6 repetitions with 5 plants per plot, totaling 30 plants per treatment. The colonies showed similar patterns of morphological characteristics for the three collection sites, with characteristics such as "circular" shape, "entire" border, "creamy" consistency, "brown" color and "bright" shine. We observed that 39.1% of the bacteria were able to perform phosphate solubilization, 61.9% performed atmospheric nitrogen fixation and 26.9% performed indole acetic acid synthesis. The results showed that the endophytic bacteria isolated from Guadua sp. did not promote dry matter accumulation of white yam plants after 45 days of cultivation in a nursery. |
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Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir).Bactérias endofíticasEndophytic bacteriaCaracterísticas macromorfológicasMacromorphological traitsBambúesReguladores del crecimientoCultivos de viverosRendimiento de los cultivosParque ZoobotânicoLago do SilêncioRio Branco (AC)Sena Madureira (AC)AcreAmazônia OcidentalWestern AmazonAmazonia OccidentalBambuRegulador de CrescimentoInhameViveiroRendimentoBamboosGuaduaGrowth regulatorsDioscorea rotundataNursery cropsCrop yieldEndophytic microorganisms have a wide genetic diversity, being very useful for agriculture, for producing growth regulators and making nutrients available to plants. In this context, this research aimed to isolate and characterize endophytic bacteria from bamboo Guadua sp., in order to evaluate the use of these microorganisms in promoting plant growth in yam plants (Dioscorea rotundata Poir). For this, the macromorphological characteristics of bacterial colonies were studied: shape, borders, consistency, color, brightness. Micromorphological analysis was performed using the Gram method. Biological tests of phosphate solubilization, biological nitrogen fixation and indole acetic acid production were carried out, where the bacteria with the best results were selected to assess the potential to promote the growth of white yam plants. The growth experiment consisted of four treatments: a control (without inoculation) and three treatments with endophytic bacteria, inoculated in pre-acclimated yam plants. Each treatment consisted of 6 repetitions with 5 plants per plot, totaling 30 plants per treatment. The colonies showed similar patterns of morphological characteristics for the three collection sites, with characteristics such as "circular" shape, "entire" border, "creamy" consistency, "brown" color and "bright" shine. We observed that 39.1% of the bacteria were able to perform phosphate solubilization, 61.9% performed atmospheric nitrogen fixation and 26.9% performed indole acetic acid synthesis. The results showed that the endophytic bacteria isolated from Guadua sp. did not promote dry matter accumulation of white yam plants after 45 days of cultivation in a nursery.Sonia Maria Lima Santos do Vale, Federal Institute of Education, Science and Technology of Acre; AMAURI SIVIERO, CPAF-AC; LAURO SARAIVA LESSA, CPAF-AC; Eduardo Pacca Luna Mattar, Federal University of Acre; Paulo Arthur Almeida do Vale, Technology Foundation of the State of Acre.VALE, S. M. L. S. doSIVIERO, A.LESSA, L. S.MATTAR, E. P. L.VALE, P. A. A. do2020-11-11T11:25:16Z2020-11-11T11:25:16Z2020-11-102020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAIMS Agriculture and Food, v. 5, n. 4, p. 850-867, 2020.2471-2086http://www.alice.cnptia.embrapa.br/alice/handle/doc/112645210.3934/agrfood.2020.4.850enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-11-11T11:25:24Zoai:www.alice.cnptia.embrapa.br:doc/1126452Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-11-11T11:25:24Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). |
title |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). |
spellingShingle |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). VALE, S. M. L. S. do Bactérias endofíticas Endophytic bacteria Características macromorfológicas Macromorphological traits Bambúes Reguladores del crecimiento Cultivos de viveros Rendimiento de los cultivos Parque Zoobotânico Lago do Silêncio Rio Branco (AC) Sena Madureira (AC) Acre Amazônia Ocidental Western Amazon Amazonia Occidental Bambu Regulador de Crescimento Inhame Viveiro Rendimento Bamboos Guadua Growth regulators Dioscorea rotundata Nursery crops Crop yield |
title_short |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). |
title_full |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). |
title_fullStr |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). |
title_full_unstemmed |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). |
title_sort |
Biotechnological potential of endophytic bacteria of bamboo Guadua sp. for promotion of growth of micropropagated yam plants (Dioscorea rotundata Poir). |
author |
VALE, S. M. L. S. do |
author_facet |
VALE, S. M. L. S. do SIVIERO, A. LESSA, L. S. MATTAR, E. P. L. VALE, P. A. A. do |
author_role |
author |
author2 |
SIVIERO, A. LESSA, L. S. MATTAR, E. P. L. VALE, P. A. A. do |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Sonia Maria Lima Santos do Vale, Federal Institute of Education, Science and Technology of Acre; AMAURI SIVIERO, CPAF-AC; LAURO SARAIVA LESSA, CPAF-AC; Eduardo Pacca Luna Mattar, Federal University of Acre; Paulo Arthur Almeida do Vale, Technology Foundation of the State of Acre. |
dc.contributor.author.fl_str_mv |
VALE, S. M. L. S. do SIVIERO, A. LESSA, L. S. MATTAR, E. P. L. VALE, P. A. A. do |
dc.subject.por.fl_str_mv |
Bactérias endofíticas Endophytic bacteria Características macromorfológicas Macromorphological traits Bambúes Reguladores del crecimiento Cultivos de viveros Rendimiento de los cultivos Parque Zoobotânico Lago do Silêncio Rio Branco (AC) Sena Madureira (AC) Acre Amazônia Ocidental Western Amazon Amazonia Occidental Bambu Regulador de Crescimento Inhame Viveiro Rendimento Bamboos Guadua Growth regulators Dioscorea rotundata Nursery crops Crop yield |
topic |
Bactérias endofíticas Endophytic bacteria Características macromorfológicas Macromorphological traits Bambúes Reguladores del crecimiento Cultivos de viveros Rendimiento de los cultivos Parque Zoobotânico Lago do Silêncio Rio Branco (AC) Sena Madureira (AC) Acre Amazônia Ocidental Western Amazon Amazonia Occidental Bambu Regulador de Crescimento Inhame Viveiro Rendimento Bamboos Guadua Growth regulators Dioscorea rotundata Nursery crops Crop yield |
description |
Endophytic microorganisms have a wide genetic diversity, being very useful for agriculture, for producing growth regulators and making nutrients available to plants. In this context, this research aimed to isolate and characterize endophytic bacteria from bamboo Guadua sp., in order to evaluate the use of these microorganisms in promoting plant growth in yam plants (Dioscorea rotundata Poir). For this, the macromorphological characteristics of bacterial colonies were studied: shape, borders, consistency, color, brightness. Micromorphological analysis was performed using the Gram method. Biological tests of phosphate solubilization, biological nitrogen fixation and indole acetic acid production were carried out, where the bacteria with the best results were selected to assess the potential to promote the growth of white yam plants. The growth experiment consisted of four treatments: a control (without inoculation) and three treatments with endophytic bacteria, inoculated in pre-acclimated yam plants. Each treatment consisted of 6 repetitions with 5 plants per plot, totaling 30 plants per treatment. The colonies showed similar patterns of morphological characteristics for the three collection sites, with characteristics such as "circular" shape, "entire" border, "creamy" consistency, "brown" color and "bright" shine. We observed that 39.1% of the bacteria were able to perform phosphate solubilization, 61.9% performed atmospheric nitrogen fixation and 26.9% performed indole acetic acid synthesis. The results showed that the endophytic bacteria isolated from Guadua sp. did not promote dry matter accumulation of white yam plants after 45 days of cultivation in a nursery. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-11T11:25:16Z 2020-11-11T11:25:16Z 2020-11-10 2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
AIMS Agriculture and Food, v. 5, n. 4, p. 850-867, 2020. 2471-2086 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126452 10.3934/agrfood.2020.4.850 |
identifier_str_mv |
AIMS Agriculture and Food, v. 5, n. 4, p. 850-867, 2020. 2471-2086 10.3934/agrfood.2020.4.850 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126452 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1817695599449866240 |